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pPD027 Reporter Template v1 (Near Spaced)

Plasmid

#138751

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD152 Reporter Template v2

Plasmid

#138752

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation. BsaI sites produce palindromic sticky ends.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD032 Reporter Template v1 (Mid Spaced)

Plasmid

#138753

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD033 Reporter Template v1 (Far Spaced)

Plasmid

#138754

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pFapO_94

Plasmid

#149597

Purpose

pXI-3-LoxP-KlURA3-pTEF1- FapO_94-yeGFP

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

yeGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1-FapO_94

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pXylO_94

Plasmid

#149598

Purpose

pXI - 3 -LoxP -KlURA3 -pTEF1 - XylO_94 -yeGFP

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

yeGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1-XylO_94

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTetO_105

Plasmid

#149599

Purpose

pXI - 3 -LoxP -KlURA3 -pTEF1 - TetO_105 -yeGFP

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

yeGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1-TetO_105

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFA271

Plasmid

#149600

Purpose

(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_183 -yeGFP

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

yeGFP

Use

Synthetic Biology

Expression

Yeast

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA272

Plasmid

#149601

Purpose

(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_182 -yeGFP

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

yeGFP

Use

Synthetic Biology

Expression

Yeast

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA197

Plasmid

#149602

Purpose

(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1 -PcaO -108 -yeGFP

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

yeGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

PcaO_1O8

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pVD_79

Plasmid

#149603

Purpose

(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - TetR

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

TetR

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pVD_01

Plasmid

#149606

Purpose

pTEF1-VanR inserted in pRS416 backbone

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

VanR

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF1

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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CRY2olig-BFP-YTHDF1(D401N)-C

Plasmid

#155354

Purpose

Lentivector to express human YTHDF1(264-559) with D401N mutation and CRY2olig-BFP at the N-terminal

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1(264-559)

Use

Lentiviral

Expression

Mammalian

Mutation

D401N

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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1xHRE-MinTK-CRE

Plasmid

#141142

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system.

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

1xHRE-MinTK-CRE

Use

Lentiviral

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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3xHRE-MinTK-CRE

Plasmid

#141143

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

3xHRE-MinTK-CRE

Use

Lentiviral

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLL3.7-Rictor-shRNA

Plasmid

#157930

Purpose

Knockdown of Rictor

Depositor

Baoji Xu

Article

Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi:

Insert

Rictor shRNA

Use

Lentiviral and RNAi

Tags

tdTomato

Promoter

mouse U6

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKM44

Plasmid

#154153

Purpose

Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Promoter

Drosophila melanogaster minimal Hsp70 promoter

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJM162

Plasmid

#134171

Purpose

Retroviral plasmid expressing GFP Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC 284-C 318AAA320 (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC 284-C 318AAA320

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pJM161

Plasmid

#134170

Purpose

Retroviral plasmid expressing GFP Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC 318AAA320 (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC 318AAA320

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJM134

Plasmid

#134165

Purpose

Retroviral plasmid expressing GFP - Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_KMLL 1-330 (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

KMLL 1-330

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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