We narrowed to 11,222 results for: SHA

1. pFUW-tetO-Myb

   Plasmid

   #139826

   Purpose

   doxycycline-inducible expression of mouse Myb in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Myb (Myb Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   P94H and Q619R- Please see depositor comment belo…

   Promoter

   TRE-mCMV

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PABPN1L

   Plasmid

   #155807

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   PABPN1L (PABPN1L Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Clim-DC2-NMM-tdtomato

   Plasmid

   #129557

   Purpose

   pClimDC-NMM:tdT cell membrane marker

   Depositor

   Inaki Ruiz-Trillo

   Article

   Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

   Insert

   N-Myristoylation motif (NMM) fused to tdtomato
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5 FRT TO-EGFP-G3BP1-S149A

   Plasmid

   #136004

   Purpose

   DOX-inducible S149A G3BP1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S149A

   Promoter

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-kanMX6-P81nmt1-GFP

   Plasmid

   #39291

   Depositor

   John Pringle, Jurg Bahler

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus with a 7bp deletion (AT…

   Promoter

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pMT2 TIA1 RRM-CFP-FRBx5

   Plasmid

   #103783

   Purpose

   expresses iPOLYMER component in mammalian cells

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

   Insert

   TIA1 RRM-CFP-FRBx5
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 6A NLRP1 CARD-FLAG WT

   Plasmid

   #163978

   Purpose

   Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVE-FH-IN

   Plasmid

   #70071

   Purpose

   2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cells

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC1300_pUB10_pco-nCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

   Plasmid

   #197981

   Purpose

   T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pco-nCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFLAG CPTP

    Plasmid

    #170742

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

    Insert

    CPTP (CPTP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET32a-Trx-PP-hNF2

    Plasmid

    #107148

    Purpose

    bacterial expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

    Insert

    neurofibromin 2 (NF2 Human)
    

    Use

    Tags

    (His)6-Thioredoxin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221 NLRP1 A66V

    Plasmid

    #166817

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    A66V

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBEST-p70a-UTR3-deGFP-6xHis-T500

    Plasmid

    #92224

    Purpose

    Expression of deGFP using the p70a promoter and UTR3

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    deGFP
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Clover-Tubulin-C-18

    Plasmid

    #56374

    Purpose

    Localization: Microtubules, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pDONR221 NLRP1 S1213A

    Plasmid

    #166824

    Purpose

    Gateway pDONR vector containing NLRP1 with the P1214R patient mutation (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    S1213A

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. MMTV-VEGF

    Plasmid

    #44625

    Depositor

    Robert Oshima

    Article

    Oshima et al Cancer Res. 2004 Jan 1;64(1):169-79.

    Insert

    VEGF121 (Vegfa Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    contains aa160-368; G165E mutation

    Promoter

    Mouse mammary tumor virus promoter

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRES

    Plasmid

    #85750

    Purpose

    Expresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Inserts

    Type II-C anti-CRISPR AcrIIC3Nme
    mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV Reep4-V5

    Plasmid

    #175120

    Purpose

    Lentiviral expression of mouse Reep4-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Reep4 (Reep4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe))

    Plasmid

    #105210

    Purpose

    Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 3 intron - see PMID: 29056342 for detailed information)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PTPN13_PDZ_1

    Plasmid

    #103919

    Purpose

    Protein expression and purification of PTN13 PDZ1 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PTPN13 (PTPN13 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. PTPN13_PDZ_3

    Plasmid

    #103921

    Purpose

    Protein expression and purification of PTN13 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PTPN13 (PTPN13 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NUDT16

    Plasmid

    #155794

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    NUDT16 (NUDT16 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOTTC1617 pAAV SYN1 Nuc-EYFP

    Plasmid

    #135567

    Purpose

    An AAV vector expressing a neuronally expressed nuclear EYFP reporter

    Depositor

    Christopher Richie

    Article

    Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

    Inserts

    empty
    Nuc-EYFP
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    hSYN1

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pfastbacHTb/mClock26-384

    Plasmid

    #47336

    Purpose

    mClock fragment cloned into pfastbacHTb vector with Hexa His tag

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    His and Tev

    Expression

    Mutation

    aa 26-384

    Promoter

    PH

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pAAV-synp-F-H2B-GCaMP6f

    Plasmid

    #102994

    Purpose

    Expresses histone fused GCaMP6f under the synapsin promoter

    Depositor

    David Cox

    Article

    Skocek et al Nat Methods. 2018 Jun;15(6):429-432. doi

    Insert

    H2B-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENTR-COIL

    Plasmid

    #16172

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    Coilin (COIL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

27. MSCV FLIP p53

    Plasmid

    #19745

    Depositor

    Richard Hynes

    Article

    Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

    Insert

    oligo targeting p53 (Trp53 Mouse)
    

    Use

    Cre/Lox, RNAi, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLN520 (SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe))

    Plasmid

    #105207

    Purpose

    Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 1 intron - see PMID: 29056342 for detailed information)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PLKO.1-UPF3B-CDS

    Plasmid

    #136045

    Purpose

    UPF3B shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GAAGCCTTGTTCCGATCTAAT)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF3B (UPF3B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTK-EdnrbE2-Sox10-FLAG-tev-AVI

    Plasmid

    #127775

    Purpose

    Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Sox10 in frame with a Flag-tag and Avi-tag

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    sox10 (SOX10 Chicken)
    

    Use

    Enhancer reporter construct

    Tags

    Avi

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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