We narrowed to 18,467 results for: Met

1. pPB9P2

   Plasmid

   #210030

   Purpose

   Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   prime editor 2
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB758

   Plasmid

   #210033

   Purpose

   Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas7-P2A-Cas5-T2A-Cas8
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCrhaB2_ApyOAHID

   Plasmid

   #226245

   Purpose

   Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI

   Depositor

   Wilfred van der Donk

   Article

   Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

   Inserts

   His6_ApyA
   ApyD
   ApyO
   ApyH
   ApyI
   

   Tags

   Hexa-Histidine

   Expression

   Bacterial

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-hSyn-ZipT-2A-IvfChr (citrine, KV2.1)

   Plasmid

   #221619

   Purpose

   A recombinant AAV2 plasmid encoding the ZipACR I151T mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-Citrine with soma targeting KV2.1 motif.

   Depositor

   John Lin

   Article

   Mermet-Joret et al eLife 2023 12:RP90327

   Insert

   ZipACR (I151V)KV2.1-2A-IvfChr-citrine-Kv2.1
   

   Use

   AAV

   Mutation

   ZipACR (I151T) with soma targeting, vfChrimson w…

   Promoter

   Human Synapsin

   Available Since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-hSyn-ZipT-2A-IvfChr (mCherry, KV2.1)

   Plasmid

   #221621

   Purpose

   A recombinant AAV2 plasmid encoding the ZipACR I151T mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-mCHerry with soma targeting KV2.1 motif.

   Depositor

   John Lin

   Article

   Mermet-Joret et al eLife 2023 12:RP90327

   Insert

   ZipACR (I151V)KV2.1-2A-IvfChr-mCherry-Kv2.1
   

   Use

   AAV

   Mutation

   ZipACR (I151T) with soma targeting, vfChrimson w…

   Promoter

   Human Synapsin

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-hSyn-ZipV-2A-IvfChr (citrine, KV2.1)

   Plasmid

   #221618

   Purpose

   A recombinant AAV2 plasmid encoding the ZipACR I151V mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-Citrine with soma targeting KV2.1 motif.

   Depositor

   John Lin

   Article

   Mermet-Joret et al eLife 2023 12:RP90327

   Insert

   ZipACR (I151V)KV2.1-2A-IvfChr-citrine-Kv2.1
   

   Use

   AAV

   Mutation

   ZipACR (I151V) with soma targeting, vfChrimson w…

   Promoter

   Human Synapsin

   Available Since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

   Plasmid

   #218916

   Purpose

   Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
   

   Use

   Lentiviral

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CCM2_I432D_eGFP

   Plasmid

   #208879

   Purpose

   Express eGFP-CCM2 with the I432D mutation in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM2 (CCM2 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   mutation I432D

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. CCM2_W421AD422A_eGFP

   Plasmid

   #208880

   Purpose

   Express eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM2 (CCM2 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   mutations W421A and D422A

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. CCM2_I428S_eGFP

    Plasmid

    #208878

    Purpose

    Express eGFP-CCM2 with the I428S mutation in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM2 (CCM2 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mutation I428S

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

    Plasmid

    #174895

    Purpose

    amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    102TAG 150TAA in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST40-CES2C-ΔC

    Plasmid

    #200555

    Purpose

    Mammalian expression of secreted mouse CES2C

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2C delta C-terminal (Ces2c Mouse)
    

    Tags

    6xHis, Flag, and V5

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-MYO10ΔF3

    Plasmid

    #194857

    Purpose

    Express EGFP-MYO10 with the F3 FERM domain lobes deleted (1-1951) in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

    Insert

    MYO10 ΔF3 (MYO10 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncated MYO10 construct. MYO10 amino acids 1-1…

    Promoter

    CMV

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAGGS-Flex/3'USS(103)-GFP

    Plasmid

    #197890

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAGGS-Flex/3'USS(553)-GFP

    Plasmid

    #197888

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CAGGS-Flex/3'USS(285)-GFP

    Plasmid

    #197889

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. deltaVP1d63C + VP2C-GFPdeg

    Plasmid

    #182481

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-GFPdeg
    deltaVP1d63C
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Linked VLP NES

    Plasmid

    #182432

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS-NES
    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Linked VLP NES (M)

    Plasmid

    #182434

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(M)-NES
    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Linked VLP NES (G)

    Plasmid

    #182476

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(G)-NES
    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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