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Ptrc::galP_NS1

Plasmid

#101843

Purpose

drives expression of galP transporter from NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Ptrc promoter driving GalP transporter expression

Use

Cloning vector

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pPICZC_CeTRIC-B2_Del

Plasmid

#87345

Purpose

Inducible expression of CeTRIC-B2 in P.Pichia strains

Depositor

Zhenfeng Liu

Article

Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

Insert

TRIC-B2

Tags

polyhistidine

Expression

Yeast

Mutation

Δ253-313

Promoter

AOX1

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAWp7-5

Plasmid

#72737

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-[miR-128 + miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-CMVp-GFP-[miR-128 + miR-132]

Use

Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-18

Plasmid

#72750

Purpose

pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-17

Plasmid

#72749

Purpose

pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-132]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-16

Plasmid

#72748

Purpose

pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-15

Plasmid

#72747

Purpose

pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-128]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-128]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-14

Plasmid

#72746

Purpose

pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-13

Plasmid

#72745

Purpose

pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-128]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-128]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-11

Plasmid

#72743

Purpose

pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-124]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-124]

Use

Lentiviral

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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c7-87/199_HLL

Plasmid

#63122

Purpose

Mm-cpn cysteine double mutant for crosslinking

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_87/199_HLL (NEWENTRY )

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S-K87C-S1…

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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c7-132/202_HLL

Plasmid

#63123

Purpose

Mm-cpn double cysteine mutant for crosslinking

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_132/202_HLL (NEWENTRY )

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S-Q132C-S…

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Myotilin-C-14

Plasmid

#55316

Purpose

Localization: Cytoskeleton, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Myotilin (MYOT Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDXNSMG3

Plasmid

#49029

Purpose

FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag, EGFP and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), EGFP, MYC, a…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDXC3MS

Plasmid

#49030

Purpose

FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, Myc tag and streptavidin binding peptide

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), MYC, and str…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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TAL2230

Plasmid

#36685

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

SDHD-TALEN_Left (sdhD Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pKB234.1

Plasmid

#170022

Purpose

Encodes LacI-controlled expression of surface-displayed hBD3 alpha-helix fragment and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
hBD3 alpha-helix

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Availability

Academic Institutions and Nonprofits only

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pKB235.4

Plasmid

#170026

Purpose

Encodes LacI-controlled expression of surface-displayed CCL20 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
CCL20 Δ alpha-helix

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Availability

Academic Institutions and Nonprofits only

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pKB236.2

Plasmid

#170029

Purpose

Encodes LacI-controlled expression of surface-displayed TCP C-terminal alpha-helix fragment and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
TCP C-terminal alpha-helix

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Availability

Academic Institutions and Nonprofits only

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pLenti_hU6-peg(ATP1A1-T804N)_mU6-BsmBI_Puro

Plasmid

#234070

Purpose

For lentiviral expression of user-specified pegRNA in tandem with epegRNA (ATP1A1-T804N) for ouabain co-selection

Depositor

Greg Findlay

Article

Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NanoLuc-LATeNT(slow)

Plasmid

#240995

Purpose

Expresses NanoLuc-LATeNT* in neurons; for AAV transduction

Depositor

Alice Ting

Article

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

NanoLuc-V5-LATeNT*

Use

AAV

Tags

V5 (internal)

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET-NT*-HRV3CP

Plasmid

#162795

Purpose

Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

Depositor

Gottfried Otting

Article

Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

Insert

Human rhinovirus 14 3C protease

Tags

NT* solubility tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Ai9

Plasmid

#22799

Purpose

Rosa26 targeting vector, Cre-dependent tdtomato expression. See article for more colors.

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

Insert

CAG-Floxed tdTomato

Use

Mouse Targeting

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLII12Pglu-700uDelta6

Plasmid

#17866

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLII12Pglu-700uDelta6

Tags

Citrine

Expression

Mammalian

Mutation

700uDelta6, ECFP insert in mglB

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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T444T

Plasmid

#113081

Purpose

Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

Depositor

Tibor Vellai

Article

Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

Type

Empty backbone

Use

RNAi

Expression

Bacterial

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pBbAW4k-loxP-TT-loxP-mRFP1

Plasmid

#134405

Purpose

Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

Depositor

Mary Dunlop

Article

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

W4-loxP-TT-loxP-mRFP1

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

W4 (modified T7A1)

Available Since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_PLK1_Pkinase

Plasmid

#109933

Purpose

Protein expression and purification of PLK1_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PLK1_Pkinase

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAJM.712

Plasmid

#108513

Purpose

PCymRC-YFP Reporter. Cuma sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PCymRC-YFP

Expression

Bacterial

Available Since

June 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.714

Plasmid

#108515

Purpose

PVanCC-YFP Reporter. Van sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PVanCC-YFP

Expression

Bacterial

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSGAb-spe

Plasmid

#122000

Purpose

A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Insert

none

Use

CRISPR

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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