We narrowed to 59,445 results for: cloning

1. pDONR221-CTSB

   Plasmid

   #158443

   Purpose

   Gateway Cloning compatible entry vector for the human CTSB gene.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   CTSB (CTSB Human)
   

   Use

   Gateway entry vector

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDB4279

   Plasmid

   #98698

   Purpose

   A control plasmid containing the intact bsdMX marker

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFH47

   Plasmid

   #125812

   Purpose

   Level0 LbCas12a, human codon optimized

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   LbCas12a, human codon optimized
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT2

   Plasmid

   #65009

   Purpose

   FRT cassette base vector

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Type

   Empty backbone

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. HDAC6 (3GV4)

   Plasmid

   #25297

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HDAC6_42 (HDAC6 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acid residues 1109-1215

   Available Since

   Aug. 16, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pEGFPpA-ACN

   Plasmid

   #20343

   Depositor

   Mario Capecchi

   Article

   Wu et al Nat Protoc. 2008 . 3(6):1056-76.

   Insert

   EGFPpA-ACN
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Available Since

   March 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAP1LC3A

   Plasmid

   #25185

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ubh67.001.121 (MAP1LC3A Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 9, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

8. CBX1

   Plasmid

   #25245

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CBX1_03 (CBX1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

9. p663-UBC-miniTurbo-V5-TTBK2_IDG-K

   Plasmid

   #189881

   Purpose

   Gateway destination clone of TTBK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   TTBK2 (TTBK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGS775

    Plasmid

    #20169

    Depositor

    Stephen West

    Article

    Dunderdale et al J Biol Chem. 1994 Feb 18. 269(7):5187-94

    Insert

    RuvC
    

    Expression

    Bacterial

    Mutation

    none

    Available Since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

11. Rat SERCA1a (pMT2)

    Plasmid

    #75182

    Purpose

    Mammalian expression of ATP2A1

    Depositor

    Jonathan Lytton

    Article

    Wu et al Am J Physiol. 1993 Feb;264(2 Pt 1):C333-

    Insert

    ATP2A1
    

    Expression

    Mammalian

    Promoter

    adenovirus major late promoter

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. HERC2 (3KCI)

    Plasmid

    #25344

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    herc2.3951.4321 (HERC2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains amino acid residues 3951-4321

    Available Since

    Aug. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pVHD

    Plasmid

    #61303

    Purpose

    Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to HA tag

    Depositor

    Julia Vorholt

    Article

    Kaczmarczyk et al Sci Rep. 2014 Sep 29;4:6453. doi: 10.103

    Type

    Empty backbone

    Tags

    HA

    Expression

    Bacterial

    Promoter

    PV10

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFH13

    Plasmid

    #125798

    Purpose

    Level0 SpCas9, wheat codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    SpCas9, wheat codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKER-mCerulean

    Plasmid

    #86622

    Purpose

    Expresses mCerulean from the EF1a promoter in mammalian cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    mCerulean
    

    Expression

    Mammalian

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. p663-UBC-miniTurbo-V5-NEK10_IDG-K

    Plasmid

    #189874

    Purpose

    Gateway destination clone of NEK10 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    NEK10 (NEK10 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p663-UBC-miniTurbo-V5-NEK4_IDG-K

    Plasmid

    #189875

    Purpose

    Gateway destination clone of NEK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    NEK4 (NEK4 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. p663-UBC-miniTurbo-V5-NEK5_IDG-K

    Plasmid

    #189876

    Purpose

    Gateway destination clone of NEK5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    NEK5 (NEK5 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. BRPF1 (3L42)

    Plasmid

    #25339

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    BRPF1_P1 (BRPF1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pICH89921

    Plasmid

    #48072

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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