We narrowed to 10,377 results for: plasmids 101

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YIplac211-VPS16-iGFPx6

Plasmid

#105275

Purpose

Plasmid for pop-in/pop-out replacement of Vps16 with Vps16-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps16 portion (VPS16 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAWH-Rluc-let-7-Poly(A)

Plasmid

#50556

Purpose

Expression of luciferase reporter mRNA in S2 cell

Depositor

Yukihide Tomari

Article

Fukaya et al Mol Cell. 2012 Dec 28;48(6):825-36. doi:

Insert

Luciferase-let7-Poly(A)

Use

Luciferase

Expression

Insect

Promoter

Actin5C

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pMST665_BB3_L 23_gRNAempty_cas9_BbsI

Plasmid

#90278

Purpose

BB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

Cas9

Use

A. niger

Expression

Bacterial

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAP608-1

Plasmid

#99484

Purpose

H2B::linker::GFP (contain 3 introns)::3XFlag::TEV::Myc::tbb-2 3UTR

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

H2B::linker::GFP with introns::3XFlag::TEV::Myc::tbb-2 3UTR

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-oChD-citrine

Plasmid

#50976

Purpose

AAV transfer vector with mammalian codon-optimized ChD-citrine between AAV2 ITR

Depositor

Roger Tsien

Article

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

oChD-citrine

Use

AAV

Tags

citrine

Mutation

ChD gene is mammalian codon optimized

Promoter

human synapsin promoter

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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B108-Cre

Plasmid

#73989

Purpose

B108 enhancer of Blimp1 gene drives Cre expression.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

B108-Cre

Expression

Mammalian

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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hSyn-DIO-PostSynBar-P2A-mCherry

Plasmid

#247719

Purpose

The set of SynBar constructs used in the optimized connectome-seq experiments.

Depositor

Boxuan Zhao

Article

Chen et al bioRxiv [Preprint]. 2025 May 26:2025.02.

Insert

PostSynBar and mCherry

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_mutCKAR3

Plasmid

#238414

Purpose

AAV construct with CKAR3 mutant expression under a human synapsin promotor and WPRE3 translation enhancer.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

phosphorylation deficient mutant of CKAR3 (T to A)

Use

AAV

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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Dm_Opt_Renilla_CSC

Plasmid

#210593

Purpose

Expresses Optimal Renilla luciferase (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal Renilla luciferase (Optimized for Drosophila, CSC metric)

Expression

Insect

Promoter

Ac5

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-Vamp2-Tag-HA

Plasmid

#218188

Purpose

To tag endogenous mouse VAMP2 with HA

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Vamp2 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJT87

Plasmid

#204489

Purpose

CAG promoted WBeta recombinase

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Insert

WBeta recombinase

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAC17V5a

Plasmid

#197413

Purpose

IPTG-inducible two-plasmid system bearing CP25 > lacO-GFP, specR, RSF1010 replicon (1/2)

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

lacO-GFP

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pJT88

Plasmid

#204490

Purpose

CAG promoted phiC31 recombinase

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Insert

phiC31 recombinase

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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aCE106

Plasmid

#204496

Purpose

Small molecule helper circuit

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

rtTA3
PhIF-ABI
VPR-Pyl1

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

hEF1a

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-EGFP

Plasmid

#196606

Purpose

Dual-Luciferase Reporter vector carrying an EGFP direct sequence

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

GFP

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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phU6 NFATC2

Plasmid

#188708

Purpose

sgRNA plasmid encoding hU6 promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pmU6 NFATC2

Plasmid

#188709

Purpose

sgRNA plasmid encoding mU6 promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p7SK NFATC2

Plasmid

#188710

Purpose

sgRNA plasmid encoding 7SK promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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phH1 NFATC2

Plasmid

#188711

Purpose

sgRNA plasmid encoding hH1 promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL1868

Plasmid

#170417

Purpose

Genetic complementation plasmid of LetAB with LetB mutant L599N

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB E. coli)

Expression

Bacterial

Mutation

LetA-LetB(L599N)

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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