We narrowed to 6,558 results for: sas;

1. pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

   Plasmid

   #156440

   Purpose

   For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   mouse SA1 (Stag1 Mouse)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVS1-TLR targeting vector

   Plasmid

   #64215

   Purpose

   Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Insert

   traffic light reporter (TLR) (AAVS1 Human)
   

   Use

   Human targeting

   Mutation

   see publication for details

   Promoter

   CAG

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

   Plasmid

   #99692

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Hbb (Hbb Synthetic)
   

   Use

   AAV

   Tags

   VPR mini

   Mutation

   dead Cas9

   Available Since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPK2101

   Plasmid

   #65770

   Purpose

   Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized SaCas9, and SaCas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS-3xFlag

   Expression

   Bacterial

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. MoFGFR3b-SV40PA

   Plasmid

   #86493

   Purpose

   mamalian cell expression of FGFR3b

   Depositor

   David Ornitz

   Article

   Chellaiah et al J Biol Chem. 1994 Apr 15;269(15):11620-7

   Insert

   FgfrR3 IIIb 12F5 (Fgfr3 Mouse)
   

   Expression

   Mammalian

   Promoter

   Moloney murine leukemia virus LTR

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcryGFP-acta1-dmdGFP

   Plasmid

   #172530

   Purpose

   alphaA-crystallin promoter (cry) drives GFP expression in the lens. The acta1 muscle-specific promoter drives expression of GFP-tagged zebrafish dmd.

   Depositor

   Joachim Berger

   Article

   Berger et al J Cell Mol Med. 2020 Jun;24(12):6680-668

   Insert

   dmd (dmd Zebrafish)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTK234_054

   Plasmid

   #123959

   Purpose

   Encodes the saCas9 CXCR1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   CXCR4 sa sgRNA 3
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mGDF8 donor CFP-hDF

   Plasmid

   #72590

   Purpose

   Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

   Depositor

   Renzhi Han

   Article

   Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

   Insert

   dysferlin (DYSF Human)
   

   Tags

   ECFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. hGDF8 donor CFP-hDF

   Plasmid

   #72591

   Purpose

   Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

   Depositor

   Renzhi Han

   Article

   Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

   Insert

   dysferlin (DYSF Human)
   

   Tags

   ECFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pONSY-CoH2B:Venus

    Plasmid

    #111877

    Purpose

    This plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Capsaspora Histone H2B (CoH2B) fused to Venus
    

    Use

    Capsaspora owczarzaki

    Tags

    Venus

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-FLAG-Co-NF-kB

    Plasmid

    #180197

    Purpose

    Expresses FLAG-tagged Capsaspora NF-kB protein in mammalian cells

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    Co-NF-kB
    

    Tags

    FLAG-tagged fusion protein

    Expression

    Mammalian

    Mutation

    aa 2-1224

    Promoter

    CMV

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGB-Co-RHD

    Plasmid

    #180207

    Purpose

    Expresses GAL4 fusion of Capsaspora NF-kB Rel Homology Domain sequences in yeast

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    Co-RHD
    

    Tags

    GAL4 (1-147) fusion

    Expression

    Yeast

    Mutation

    aa 2-581

    Promoter

    AdhI

    Available Since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGB-Co-Cterm

    Plasmid

    #180208

    Purpose

    Expresses GAL4 fusion of Capsaspora NF-kB C-terminal sequences in yeast

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    Co-RHD
    

    Tags

    GAL4 (1-147) fusion

    Expression

    Yeast

    Mutation

    aa 542-1224

    Promoter

    AdhI

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-FLAG-Co-NF-kB-Cterm

    Plasmid

    #180199

    Purpose

    Expresses FLAG-tagged Capsaspora NF-kB C-terminal sequences in mammalian cells

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    Co-NF-kBCterm
    

    Tags

    FLAG-tagged fusion protein

    Expression

    Mammalian

    Mutation

    aa 542-1224

    Promoter

    CMV

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJP102

    Plasmid

    #176480

    Purpose

    Expresses mScarlet under control of the Capsaspora EF1 promoter and NatR under control of the Capsaspora actin promoter

    Depositor

    Duojia Pan

    Article

    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

    Inserts

    actin > NatR
    EF1 > mScarlet
    

    Use

    Capsaspora owczarzaki expression

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJP103

    Plasmid

    #176481

    Purpose

    Expresses mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

    Depositor

    Duojia Pan

    Article

    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

    Inserts

    actin > HygR
    EF1 > mScarlet
    

    Use

    Capsaspora owczarzaki expression

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJP72

    Plasmid

    #176479

    Purpose

    Expresses mScarlet under control of the Capsaspora EF1 promoter and GenR under control of the Capsaspora actin promoter

    Depositor

    Duojia Pan

    Article

    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

    Inserts

    actin > GenR
    EF1 > mScarlet
    

    Use

    Capsaspora owczarzaki expression

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGB-Co-NF-kB

    Plasmid

    #180206

    Purpose

    Expression GAL4-fusion of Co-NF-kB in yeast

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    Co-NF-kB
    

    Tags

    GAL4 (1-147) fusion

    Expression

    Yeast

    Mutation

    aa 2-1224

    Promoter

    AdhI

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-FLAG-Co-RHD

    Plasmid

    #180198

    Purpose

    Expresses FLAG-tagged version of the Co-NF-kB Rel Homology Domain

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    Co-RHD
    

    Tags

    FLAG-tagged fusion protein

    Expression

    Mammalian

    Mutation

    aa 2-582

    Promoter

    CMV

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pONSY-CoNMM:mCherry

    Plasmid

    #111878

    Purpose

    This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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