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AZ64_pE.DonorCLYBL.TS

Plasmid

#199228

Purpose

Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

Insert

Expression unit for EGFP reporter

Use

Gene targeting donor plasmid

Expression

Mammalian

Promoter

Human PGK1 gene promoter

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDL278_P23-DsRed-Express2

Plasmid

#121505

Purpose

E. coli-streptococcal shuttle vector that expresses rfp

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

DsRed-Express2

Use

E. coli streptococcal shuttle vector

Promoter

P23

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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px-458-sgRNA-scramble

Plasmid

#206924

Purpose

Plasmid expressing Cas9, GFP and non-targeting guides for using as a control in CRISPR experiments

Depositor

José Cuezva

Article

Romero-Carraminana et al Commun Biol. 2023 Aug 12;6(1):836. doi:

Insert

non-targeting human sgRNA guides

Use

CRISPR

Promoter

U6

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2

Plasmid

#132775

Purpose

Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE2

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-Golgi eGFP

Plasmid

#79809

Purpose

To express and target GFP into the Golgi apparatus using lentiviruses

Depositor

Pantelis Tsoulfas

Insert

Golgi eGFP (B4GALT1 Human)

Use

Lentiviral

Tags

Fusion of the first 62 amino acids of the human b…

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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STAT3-GFP

Plasmid

#110495

Purpose

reporter of STAT3 signaling activity

Depositor

Michael Lewis

Article

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

4 repeats of STAT3 binding site M67 at GFP promoter region

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19 - T7 pro - IRES - EGFP

Plasmid

#138586

Purpose

Express EGFP with an upstream T7 promoter in pUC19 backbone

Depositor

Fei Chen

Article

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

EGFP

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

Plasmid

#196703

Purpose

Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 D210N

Expression

Mammalian

Mutation

D210N, catalytically inactive

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-GFP-Tmem192

Plasmid

#134630

Purpose

lysosomal marker

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

FLAG and GFP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-UTX

Plasmid

#24168

Purpose

Mammalian expression of human UTX with HA tag

Depositor

Kristian Helin

Article

Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

Insert

UTX (KDM6A Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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