We narrowed to 12,476 results for: pcdna

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pcDNA3.1-Hyg-BFP-P2A-otPKR-FL

Plasmid

#214360

Purpose

Expression of the Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmon

Depositor

Bertrand Collet

Insert

BFP-P2A-otPKRFL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo-BFP-P2A-otPKR-FL

Plasmid

#214364

Purpose

Expression of the Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmon

Depositor

Bertrand Collet

Article

Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

Insert

BFP-P2A-otPKRFL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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3'-BirA*-FLAG minimal CMV pCDNA5

Plasmid

#212047

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Myc-E3(1) C885A-CUbo

Plasmid

#212800

Purpose

Constitutive/Doxycycline-inducible expression of Myc-E3(1) C885A-CUbo in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Tags

CUbo and Myc

Expression

Mammalian

Mutation

HOIP(C885A)

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-mCherry-FLAG

Plasmid

#212798

Purpose

Constitutive/Doxycycline-inducible expression of NUbo-mCherry-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Tags

mCherry-FLAG

Expression

Mammalian

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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5'-BirA*-FLAG minimal CMV pCDNA5

Plasmid

#212046

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG GFP minimal CMV pCDNA5

Plasmid

#212086

Purpose

LR vector for integration of GFP into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

GFP

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-H2B-His8

Plasmid

#212810

Purpose

Constitutive or doxycycline-inducible expression of NUbo-H2B-His8 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Tags

8xHis and NUbo

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-His8-H2B-CUbo

Plasmid

#212812

Purpose

Constitutive or doxycycline-inducible expression of His8-H2B-CUbo in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

H2B

Tags

8xHis and CUbo

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo-GFP

Plasmid

#212815

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo-GFP in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Human, Budding Yeast)

Tags

8xHis and CUbo-GFP

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo-His6

Plasmid

#212819

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo-His6 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Human, Budding Yeast)

Tags

CUbo-6xHis

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo(K63R)

Plasmid

#212822

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R) in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Human, Budding Yeast)

Tags

CUbo(K63R)

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(ERED198-201AAAA)-3xFLAG-pCDNA

Plasmid

#201965

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

E198A, R199A,E200A, D201A

Available Since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(RKRKR232-236AAAAA)-3xFLAG-pCDNA

Plasmid

#201967

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

R232A, K233A, R234A, K235A, R236A

Available Since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 myc-Rab10-mito

Plasmid

#208367

Purpose

Mammalian expression of myc-tagged human Rab10 with C-terminal mito signal

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

myc-Rab10-mito (RAB10 Human)

Expression

Mammalian

Mutation

wildtype

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NKX6-2-Venus S246A/T252A

Plasmid

#209180

Purpose

Mammalian expression of human NKX6-2 T252D phosphoresistant mutant

Depositor

Federico Herrera

Article

Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

Insert

NKX6-2 (S246A/T252A) fused with Venus (NKX6-2 Human)

Tags

Venus

Expression

Mammalian

Mutation

NKX6-2 (S246A/T252D)

Promoter

CMV

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-BT-FLAG

Plasmid

#205621

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

deleted BT domain

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-KD-FLAG

Plasmid

#205626

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

kinase-dead (KD; K271H)

Promoter

CMV

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del1-17)-FLAG

Plasmid

#204533

Purpose

Mammalian expression of human CALR ins5 (del1-17)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del1-17)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del1-17)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3(+)-UFBP1 F196A V198A-MYC

Plasmid

#204556

Purpose

Expresses FLAG-tagged UFBP1 F196A V198A in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Mammalian

Mutation

F196A V198A

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(I)

Plasmid

#204700

Purpose

This plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-p300(I) (BRD4, EP300 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

deletion of all TF-bidning domains and HAT in p300

Promoter

CMV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(HI)

Plasmid

#204699

Purpose

This plasmid allows for inducible expression of engineered BRD4-p300(HI) fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-p300 (BRD4, EP300 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

deletion of all TF-bidning domains in p300

Promoter

CMV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(H*IT)

Plasmid

#204698

Purpose

This plasmid allows for inducible expression of engineered BRD4-p300(H*IT) fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-p300(H*IT) (BRD4, EP300 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

D1399Y substitution in p300

Promoter

CMV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (277-357)

Plasmid

#203434

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (116-439)

Plasmid

#203425

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (201-298)

Plasmid

#203431

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (208-300)

Plasmid

#203432

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG-NAD-NLS3x

Plasmid

#193822

Purpose

CMV overexpression of ChemoG-NAD in the nucleus of mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-NAD-NLS3x

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO-ChemoL-NAD-NLS3x

Plasmid

#193828

Purpose

CMV overexpression of ChemoL-NAD in the nucleus of mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoL-NAD-NLS3x

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO-[4xCox8]-ChemoL-NAD

Plasmid

#193829

Purpose

CMV overexpression of ChemoL-NAD in the mitochondria of mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

[4xCox8]-ChemoL-NAD

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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