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Showing: 10851 - 10900 of 24790 results

  12. pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

    Plasmid
    #101235
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant F491A/M495A/T496A/N497A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    F491A, M495A, T496A, N497A and Q926A
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101235-plasmid-map-sequence-id-198037

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  13. pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

    Plasmid
    #101234
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant T657A/G658A/W659A/R661A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    T657A, G658A, W659A, R661A and Q926A
    Promoter
    T7
    Available Since
    Nov. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    101234-plasmid-map-sequence-id-198016

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  14. pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Plasmid
    #101220
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant N692A/M694A/Q695A/H698A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    N692A, M694A, Q695A, H698A and Q926A
    Promoter
    T7
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    101220-plasmid-map-sequence-id-197535

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  15. pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Plasmid
    #101232
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    G528A, V583A, E584A, D585A, N588A and Q926A
    Promoter
    T7
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101232-plasmid-map-sequence-id-197212

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  34. qTAG-C-mStayGold-Puro-H2BC11-MMEJ

    Plasmid
    #227333
    Purpose
    MMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227333-plasmid-map-sequence-id-452137

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  35. qTAG-C-Solo-miRFP670nano3-H2BC11

    Plasmid
    #227331
    Purpose
    Donor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a miRFP670nano3 Tag (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227331-plasmid-map-sequence-id-452128

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  36. qTAG-C-Solo-mStayGold-H2BC11

    Plasmid
    #227330
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mStayGold Tag (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227330-plasmid-map-sequence-id-452124

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  37. qTAG-C-mStayGold-EFS-Puro-PLK4

    Plasmid
    #227311
    Purpose
    Donor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    PLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227311-plasmid-map-sequence-id-452079

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  38. qTAG-N-Solo-mStayGold-PCNT

    Plasmid
    #227285
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the PCNT locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PCNT (Addgene #227284)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    PCNT Homology Arms flanking a mStayGold Tag (PCNT Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227285-plasmid-map-sequence-id-452054

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  48. AA246

    Plasmid
    #215946
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD55_v1 [Sp]; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215946-plasmid-map-sequence-id-431539

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  49. AA233

    Plasmid
    #215941
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD274_v1 [Sp]; trRNA_v14 [Sp]
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215941-plasmid-map-sequence-id-431532

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  50. AA244

    Plasmid
    #215945
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; sgCD59_v1 [Sp]; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215945-plasmid-map-sequence-id-431525

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Showing: 10851 - 10900 of 24790 results