We narrowed to 17,632 results for: URE

Showing: 10851 - 10900 of 17632 results

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DYNLRB2

Plasmid

#132534

Purpose

Human Rbl-2, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLRB2 (DYNLRB2 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFP-RIP2_3K

Plasmid

#131205

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 K209/410/538R (RIPK2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

K209/410/538R

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA105

Plasmid

#128521

Purpose

Expresses amyloid human Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Article

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme T70N (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-Cdc45 (delta 154–164)

Plasmid

#126879

Purpose

Expresses crystallisation construct of human Cdc45 in bacteria

Depositor

Luca Pellegrini

Article

Simon et al Nat Commun. 2016 May 18;7:11638. doi: 10

Insert

Human Cdc45 (CDC45 Human)

Tags

No tag

Expression

Bacterial

Mutation

deletion amino acid 154-164

Promoter

T7 promoter

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 d835

Plasmid

#113959

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-834

Promoter

cmv

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107321

Purpose

Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1333S) is fused to SaCas9

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)

Plasmid

#115432

Purpose

Vector for expressing RPL28 with mutagenized exon2

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

exon 2 deleted, with MfeI / NheI cloning site

Promoter

Ashbya gospii TEF1 and endogenous

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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Native promoter dCas9

Plasmid

#113656

Purpose

dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

Depositor

Jeffrey Barrick

Article

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

Native promoter and dCas9

Use

CRISPR

Expression

Bacterial

Promoter

S. pyogenes Cas9 native promoter

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 P294A

Plasmid

#112102

Purpose

Mammalian expression of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

P294A

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pOU2

Plasmid

#41754

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc Fly, E. coli)

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C45S C338S C451S

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(WT)

Plasmid

#112720

Purpose

Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(WT)

Tags

6His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P46A)

Plasmid

#112721

Purpose

This mutation (P46A) marks the first residue within the evolved region of U1A.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P46A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P46A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q48T)

Plasmid

#112725

Purpose

This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48T)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48T

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P51A)

Plasmid

#112728

Purpose

Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P51A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P51A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q54A)

Plasmid

#112730

Purpose

Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q54A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q54A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgAMDHD1

Plasmid

#102316

Purpose

genetic depletion of AMDHD1

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgAMDHD1 (AMDHD1 Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pmiR-report-hARPC2

Plasmid

#112549

Purpose

3'UTR reporter assay

Depositor

Shusheng Wang

Article

Li et al Sci Rep. 2017 Apr 19;7(1):915. doi: 10.1

Insert

ARPC2 (ARPC2 Human)

Use

Luciferase

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_BZRAP1-2/3

Plasmid

#91551

Purpose

Protein expression and purification of human SH3 domain construct BZRAP1-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BZRAP1-2/3 (TSPOAP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF2-2/3

Plasmid

#91557

Purpose

Protein expression and purification of human SH3 domain construct SH3RF2-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF2-2/3 (SH3RF2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_NOXO1-2/2

Plasmid

#91558

Purpose

Protein expression and purification of human SH3 domain construct NOXO1-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NOXO1-2/2 (NOXO1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_BZRAP1-3/3

Plasmid

#91559

Purpose

Protein expression and purification of human SH3 domain construct BZRAP1-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BZRAP1-3/3 (TSPOAP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_GRAP-2/2

Plasmid

#91529

Purpose

Protein expression and purification of human SH3 domain construct GRAP-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRAP-2/2 (GRAP Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_PIK3R2-1/1

Plasmid

#91535

Purpose

Protein expression and purification of human SH3 domain construct PIK3R2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PIK3R2-1/1 (PIK3R2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only
pHH0103_NCK2-1/3-SV1/2

Plasmid

#91538

Purpose

Protein expression and purification of human SH3 domain construct NCK2-1/3-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK2-1/3-SV1/2 (NCK2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_MPP4-1/1-IS3/3

Plasmid

#91519

Purpose

Protein expression and purification of human SH3 domain construct MPP4-1/1-IS3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MPP4-1/1-IS3/3 (MPP4 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_BZRAP1-1/3

Plasmid

#91487

Purpose

Protein expression and purification of human SH3 domain construct BZRAP1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BZRAP1-1/3 (TSPOAP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_NCF2-2/2

Plasmid

#91488

Purpose

Protein expression and purification of human SH3 domain construct NCF2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCF2-2/2 (NCF2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_NCF1-2/2

Plasmid

#91492

Purpose

Protein expression and purification of human SH3 domain construct NCF1-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCF1-2/2 (NCF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_TJP2-1/1

Plasmid

#91493

Purpose

Protein expression and purification of human SH3 domain construct TJP2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TJP2-1/1 (TJP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_NCF2-1/2

Plasmid

#91479

Purpose

Protein expression and purification of human SH3 domain construct NCF2-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCF2-1/2 (NCF2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RIMBP2-2/3

Plasmid

#91468

Purpose

Protein expression and purification of human SH3 domain construct RIMBP2-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP2-2/3 (RIMBP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_MYO7A-1/1

Plasmid

#91446

Purpose

Protein expression and purification of human SH3 domain construct MYO7A-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MYO7A-1/1 (MYO7A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_TNK1-1/1-SV3/3

Plasmid

#91451

Purpose

Protein expression and purification of human SH3 domain construct TNK1-1/1-SV3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TNK1-1/1-SV3/3 (TNK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_TNK1-1/1-SV2/3

Plasmid

#91425

Purpose

Protein expression and purification of human SH3 domain construct TNK1-1/1-SV2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TNK1-1/1-SV2/3 (TNK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_NOXA1-2/2-SV2/2

Plasmid

#91433

Purpose

Protein expression and purification of human SH3 domain construct NOXA1-2/2-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NOXA1-2/2-SV2/2 (NOXA1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SH3RF2-1/3-SV2/2

Plasmid

#91408

Purpose

Protein expression and purification of human SH3 domain construct SH3RF2-1/3-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF2-1/3-SV2/2 (SH3RF2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RIMBP2-3/3

Plasmid

#91411

Purpose

Protein expression and purification of human SH3 domain construct RIMBP2-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP2-3/3 (RIMBP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_GRAP-1/2

Plasmid

#91412

Purpose

Protein expression and purification of human SH3 domain construct GRAP-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRAP-1/2 (GRAP Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_TRIP10-1/1

Plasmid

#91419

Purpose

Protein expression and purification of human SH3 domain construct TRIP10-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TRIP10-1/1 (TRIP10 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_NCKIPSD-1/1

Plasmid

#91376

Purpose

Protein expression and purification of human SH3 domain construct NCKIPSD-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCKIPSD-1/1 (NCKIPSD Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SH3KBP1-2/3

Plasmid

#91378

Purpose

Protein expression and purification of human SH3 domain construct SH3KBP1-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3KBP1-2/3 (SH3KBP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_NOXA1-2/2-SV1/2

Plasmid

#91386

Purpose

Protein expression and purification of human SH3 domain construct NOXA1-2/2-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NOXA1-2/2-SV1/2 (NOXA1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RIMBP2-1/3

Plasmid

#91388

Purpose

Protein expression and purification of human SH3 domain construct RIMBP2-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP2-1/3 (RIMBP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SH3PXD2B-3/4

Plasmid

#91359

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2B-3/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2B-3/4 (SH3PXD2B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SPTA1-1/1

Plasmid

#91363

Purpose

Protein expression and purification of human SH3 domain construct SPTA1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SPTA1-1/1 (SPTA1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RIMBP3-3/3

Plasmid

#91365

Purpose

Protein expression and purification of human SH3 domain construct RIMBP3-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP3-3/3 (RIMBP3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RIMBP3-2/3

Plasmid

#91370

Purpose

Protein expression and purification of human SH3 domain construct RIMBP3-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP3-2/3 (RIMBP3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SH3GLB2-1/1

Plasmid

#91337

Purpose

Protein expression and purification of human SH3 domain construct SH3GLB2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3GLB2-1/1 (SH3GLB2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SGSM3-1/1

Plasmid

#91348

Purpose

Protein expression and purification of human SH3 domain construct SGSM3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SGSM3-1/1 (SGSM3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SH3PXD2B-2/4

Plasmid

#91316

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2B-2/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2B-2/4 (SH3PXD2B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 17,632 results for: URE