We narrowed to 10,545 results for: Uty
-
Plasmid#174599PurposeExpresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia LabDepositorInsertextracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)
ExpressionMammalianAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAF123
Plasmid#135921PurposeAll-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.DepositorTypeEmpty backboneUseTransposonExpressionMammalianAvailable SinceJan. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2beta WT GFP
Plasmid#128820PurposeTo visualize Doc2beta WT in PC12 cellsDepositorAvailable SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4max-NRRH
Plasmid#136918PurposeExpresses BE4max-NRRH in mammalian cellsDepositorInsertBE4max-NRRH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2alpha WT GFP
Plasmid#128819PurposeTo visualize Doc2alpha WT in PC12 cellsDepositorAvailable SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB-2
Plasmid#210388PurposeAll-in-one cumate-based inducible CRISPRi plasmid for use in StreptomycesDepositorInsertsdCas9
CymR
sgRNA cassette
UseCRISPR; Integrative vector for use in streptomycesMutationBsaI-flanked RFP cassette inserted in place of sp…PromoterSP11 and SP30Available SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3myc-PR-LBD
Plasmid#89113Purposemammalian expression of human progesterone receptor ligand binding domain amino acid residues 632-933 with N-terminal myc tagDepositorInserthuman progesterone receptor ligand binding domain (PGR Human)
TagsmycExpressionMammalianMutationcontains Ligand Binding Domain (632-933)PromoterCMVAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET41-MBP-t-A3A-t-His
Plasmid#109231PurposeBacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV proteaseDepositorInserthuman APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)
TagsHis and MBPExpressionBacterialAvailable SinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP
Plasmid#192273PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloningDepositorInsertCymR
UseCRISPRExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-C_sfYFPTwinStrep
Plasmid#45944Purposeexpresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequenceDepositorInsertsynthetic sfYFP (codon usage adapted to P.putida KT2440)
TagsPelB export sequence and Twin-Strep-tagExpressionBacterialPromoterlacIq/PtrcAvailable SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK cyclin T1 (P#468)
Plasmid#14607DepositorAvailable SinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
His-MBP_G4G6 in pRSF
Plasmid#188454PurposeExpress in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.DepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM20C
Plasmid#135487PurposeAgrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hphDepositorInserthph SpunH2A/Bpr H2B-tdTomato
TagstdTomatoExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE 3xHA-MKK6-EE puro
Plasmid#216791PurposeMammalian expression of constitutively active human MKK6DepositorInsertMKK6 (MAP2K6 Human)
UseRetroviralTags3x-HAExpressionMammalianMutationS207E and T211E, constitutively activePromoterLTRAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
myc-Miro1 (lacking TM)
Plasmid#127613PurposeHuman Miro1 with the transmembrane deletedDepositorAvailable SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV-NLS-ISDge10-NLS-3xHA-bGHpA;U6-BsaI-reRNA
Plasmid#205318PurposeAll-in-one AAV vector expressing ISDge10 TnpB and its reRNADepositorInserthumanized ISDge10 TnpB
UseAAVTags3 x HAExpressionMammalianAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDawn-Ag43
Plasmid#107742PurposeExpresses Ag43 under the control of a blue-light transcriptional regulatorDepositorInsertAg43 (flu )
ExpressionBacterialMutationremoved all pstI cut sites with silent mutations,…PromoterpLambdaAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-UAP56 WT
Plasmid#110125PurposeExpresses Flag-tagged human UAP56 (DDX39B)DepositorAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-MjDim1
Plasmid#189936PurposeExpression of Mjdim1, a methyltransferase used in m6A-SAC-seq.DepositorInsertrsmA (MJ_RS00455 Methanocaldococcus jannaschii)
Tags6x His tag, FLAG tag, SUMO tagExpressionBacterialPromoterT7 promoterAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCAG eGFP T2A SP HALO HAPLN1
Plasmid#228200PurposeAAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoterDepositorAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only