We narrowed to 23,099 results for: crispr

1. pDonor-PRF1-T2A-cDNA

   Plasmid

   #209076

   Purpose

   Targeting vector for the human PRF1 locus to replace exon 3 with repaired cDNA

   Depositor

   Klaus Rajewsky

   Article

   Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

   Insert

   PRF1-T2A-cDNA HDRT (PRF1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. qTAG-N-Blast-moxGFP-LMNB1

   Plasmid

   #207772

   Purpose

   Donor template for Blast-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-moxGFP Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pKLV2-U6gRNA5(ZNF649_5-1)-PGKpuroBFP-W

   Plasmid

   #212000

   Purpose

   Express gRNA against ZNF649 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting ZNF649 (ZNF649 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKLV2-U6gRNA5(ZNF649_5-5)-PGKpuroBFP-W

   Plasmid

   #212001

   Purpose

   Express gRNA against ZNF649 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting ZNF649 (ZNF649 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKLV2-U6gRNA5(ZNF90_5-3)-PGKpuroBFP-W

   Plasmid

   #212002

   Purpose

   Express gRNA against ZNF90 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting ZNF90 (ZNF90 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKLV2-U6gRNA5(CTRL00509)-PGKpuroBFP-W

   Plasmid

   #211956

   Purpose

   Express non-cutter control gRNA with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   non-cutter control sgRNA_CTRL00509
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV2-U6gRNA5(CTRL00626)-PGKpuroBFP-W

   Plasmid

   #211957

   Purpose

   Express non-cutter control gRNA with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   non-cutter control sgRNA_CTRL00626
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

   Plasmid

   #189633

   Purpose

   Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. qTAG-N-Blast-miniTurbo-LMNB1

   Plasmid

   #207774

   Purpose

   Donor template for Blast-2A-miniTurbo insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-miniTurbo Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. qTAG-N-Blast-V5-LMNB1

    Plasmid

    #207779

    Purpose

    Donor template for Blast-2A-V5 insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Blast-V5 Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. qTAG-C-Solo-mScarlet-H3C2

    Plasmid

    #207781

    Purpose

    Donor template for mScarlet insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a mScarlet Tag (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. qTAG-C-mNeon-Blast-H3C2

    Plasmid

    #207782

    Purpose

    Donor template for mNeon-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a mNeon-Blast Cassette (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro

    Plasmid

    #189636

    Purpose

    Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

    Plasmid

    #189632

    Purpose

    Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. qTAG-N-Solo-mScarlet-ACTB

    Plasmid

    #207750

    Purpose

    Donor template for mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a mScarlet Tag (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. qTAG-N-Solo-mNeon-ACTB

    Plasmid

    #207749

    Purpose

    Donor template for mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a mNeon Tag (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. qTAG-N-Blast-moxGFP-TUBA1B

    Plasmid

    #207766

    Purpose

    Donor template for Blast-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Blast-moxGFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pMMK ENTR 2 hU6 ACTB sgRNA

    Plasmid

    #206270

    Purpose

    ENTR Vector 2 for MultiSite Gateway assembly. Encodes hU6 hACTB sgRNA

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    ACTB sgRNA
    

    Use

    CRISPR; Multimate/gateway entr 2

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMMK ENTR 2 hU6 HEK3 PegRNA

    Plasmid

    #206277

    Purpose

    ENTR Vector 2 for MultiSite Gateway assembly. Encodes HEK3 PegRNA

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    HEK3 PegRNA
    

    Use

    CRISPR; Multimate/gateway entr 2

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. OA-1085L

    Plasmid

    #191376

    Purpose

    pBac-U6-crGFP-array-UTR-opie2-eGFP-3xp3-tdTomato

    Depositor

    Omar Akbari

    Article

    Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

    Insert

    4 gRNAs targeting GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. OA-1093B

    Plasmid

    #194003

    Purpose

    pBac-U6-crCHIKV-array-UTR-3xp3-tdTomato

    Depositor

    Omar Akbari

    Article

    Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

    Insert

    4 gRNAs targeting CHIKV
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. OA-1085F

    Plasmid

    #191375

    Purpose

    pBac-U6-crYellow-array-UTR-3xp3-tdTomato

    Depositor

    Omar Akbari

    Article

    Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

    Insert

    4 gRNAs targeting yellow
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pShuttle.CC9

    Plasmid

    #192931

    Purpose

    Gateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expression

    Depositor

    Thorsten Stiewe

    Article

    Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

    Type

    Empty backbone

    Use

    Gateway-compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYPQ131-RZ-Cas12j2

    Plasmid

    #189782

    Purpose

    Golden Gate entry vector to clone the 1st Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYPQ132-RZ-Cas12j2

    Plasmid

    #189783

    Purpose

    Golden Gate entry vector to clone the 2nd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYPQ134-RZ-Cas12j2

    Plasmid

    #189785

    Purpose

    Golden Gate entry vector to clone the 4th Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 2xNLS-NLP-cMyc AspCas12a

    Plasmid

    #182120

    Purpose

    pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    2xNLS-NLP-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a

    Plasmid

    #182126

    Purpose

    pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

    Plasmid

    #182121

    Purpose

    pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    1N/2CxNLS-cMyc-NLP-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a

    Plasmid

    #182124

    Purpose

    pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJJGL003

    Plasmid

    #180607

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertion

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    DpbCasX with R3 loop
    

    Use

    CRISPR

    Tags

    10x His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNOC_dCas9_sgRNA Td2

    Plasmid

    #176258

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pNOC_dCas9_sgRNA Td1

    Plasmid

    #176257

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNOC_dfnCas12a_crRNA Td2

    Plasmid

    #176255

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNOC_hfnCas12a-Nlux_crRNA NR2

    Plasmid

    #176245

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pNOC_hfnCas12a_crRNA NR2

    Plasmid

    #176248

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pNOC_hfnCas12a_crRNA NR1

    Plasmid

    #176247

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNOC_hfnCas12a-Nlux_crRNA NR3

    Plasmid

    #176246

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pNOC_hfnCas12a_crRNA NR3

    Plasmid

    #176249

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pPPC002

    Plasmid

    #171139

    Purpose

    For integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J1-BBa_J23117-sfGFP
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    J1-BBa_J23117 and Sp.pCas9, BBa_J23107

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pUFlip-floxed2A-KalTA4-; gcry1:BFP -2

    Plasmid

    #173891

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; gcry1: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pUPD2 sgRNA sCRNA 2.0 (GB2461)

    Plasmid

    #160593

    Purpose

    Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    sgRNA sCRNA 2.0
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

    Plasmid

    #160570

    Purpose

    A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    sgRNA:scaffold tetraloop MS2 aptamer SAM
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pKLV-gRNA-Bcan-Ntrk1_4

    Plasmid

    #136413

    Purpose

    Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Insert

    U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pKLV-gRNA-Myb-Qk_1

    Plasmid

    #136415

    Purpose

    Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Insert

    U6_sgRNA(Myb)_U6_sgRNA(Qk)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. gh105

    Plasmid

    #106775

    Purpose

    expression of gRNA targeting B3GALT1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    B3GALT1 (B3GALT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. gh2

    Plasmid

    #106686

    Purpose

    expression of gRNA targeting GALNT2

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    GALNT2 (GALNT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. gh110

    Plasmid

    #106769

    Purpose

    expression of gRNA targeting B3GALTL (B3GLCT)

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    B3GALTL (B3GLCT) (B3GLCT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. gh106

    Plasmid

    #106776

    Purpose

    expression of gRNA targeting B3GALT2

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    B3GALT2 (B3GALT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. gh109

    Plasmid

    #106768

    Purpose

    expression of gRNA targeting B3GALT6

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    B3GALT6 (B3GALT6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge