We narrowed to 8,688 results for: APP

1. BS-ZJLEU-PTEF1-HFL1-W371A-GFP

   Plasmid

   #207019

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   W371A

   Promoter

   pTEF1

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ypq2-2GFP-URA

   Plasmid

   #207010

   Purpose

   Integration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   YPQ2
   

   Use

   Tags

   2xGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Vph1-2GFP-URA

   Plasmid

   #207009

   Purpose

   Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   VPH1
   

   Use

   Tags

   2xGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRhaCAST

   Plasmid

   #211791

   Purpose

   pZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoter

   Depositor

   Silvia Cardona

   Article

   Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Rhamnose-inducible

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMAT_M1_YLVV

   Plasmid

   #197081

   Purpose

   This plasmid contains the coding sequence for signaling peptide M1, which contains the YLVV signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

   Depositor

   Wendell Lim

   Article

   Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

   Insert

   This plasmid contains the coding sequence for signaling peptide M1, which contains the YLVV signaling motif.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAT_M2_YVPM

   Plasmid

   #197082

   Purpose

   This plasmid contains the coding sequence for signaling peptide M2, which contains the YVPM signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

   Depositor

   Wendell Lim

   Article

   Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

   Insert

   This plasmid contains the coding sequence for signaling peptide M2, which contains the YVPM signaling motif.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAT_M3_YENL

   Plasmid

   #197083

   Purpose

   This plasmid contains the coding sequence for signaling peptide M3, which contains the YENL signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

   Depositor

   Wendell Lim

   Article

   Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

   Insert

   This plasmid contains the coding sequence for signaling peptide M3, which contains the YENL signaling motif.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMAT_M5_YESP

   Plasmid

   #197085

   Purpose

   This plasmid contains the coding sequence for signaling peptide M5, which contains the YESP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

   Depositor

   Wendell Lim

   Article

   Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

   Insert

   This plasmid contains the coding sequence for signaling peptide M5, which contains the YESP signaling motif.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMAT_M6_ITYAAV

   Plasmid

   #197086

   Purpose

   This plasmid contains the coding sequence for signaling peptide M6, which contains the ITYAAV signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

   Depositor

   Wendell Lim

   Article

   Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

   Insert

   This plasmid contains the coding sequence for signaling peptide M6, which contains the ITYAAV signaling motif.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAT_M7_PVQP

    Plasmid

    #197087

    Purpose

    This plasmid contains the coding sequence for signaling peptide M7, which contains the PVQP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

    Depositor

    Wendell Lim

    Article

    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

    Insert

    This plasmid contains the coding sequence for signaling peptide M7, which contains the PVQP signaling motif.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMAT_M8_RSTKP

    Plasmid

    #197088

    Purpose

    This plasmid contains the coding sequence for signaling peptide M8, which contains the RSTKP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

    Depositor

    Wendell Lim

    Article

    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

    Insert

    This plasmid contains the coding sequence for signaling peptide M8, which contains the RSTKP signaling motif.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMAT_M9_PVQE

    Plasmid

    #197091

    Purpose

    This plasmid contains the coding sequence for signaling peptide M9, which contains the PVQE signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

    Depositor

    Wendell Lim

    Article

    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

    Insert

    This plasmid contains the coding sequence for signaling peptide M9, which contains the PVQE signaling motif.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. RC(14-3-3ε)-LgBit

    Plasmid

    #208926

    Purpose

    Recognition domain for in vitro RSK-activity sensor

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    14-3-3ε (YWHAQ Human)
    

    Use

    Tags

    HISx6, LgBit, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. ClhN-P1K-3VSV-Atg8-MET15

    Plasmid

    #207055

    Purpose

    Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    Promoter

    pATG8

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. P1K-3HA-Atg8∆R-MET15

    Plasmid

    #207034

    Purpose

    Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    ∆R

    Promoter

    pATG8

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACYC Destination

    Plasmid

    #207675

    Purpose

    Trio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).

    Depositor

    Ramon Gonzalez

    Article

    Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CymRC, PhlF, VanCC

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRAPa(E162A,M164A,R167A)-3xFLAG-pCDNA

    Plasmid

    #201964

    Purpose

    Mammalian expression of mutated Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Use

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Mutation

    E162A,M164A,R167A

    Promoter

    Available since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRAPa(ERED198-201AAAA)-3xFLAG-pCDNA

    Plasmid

    #201965

    Purpose

    Mammalian expression of mutated Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Use

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Mutation

    E198A, R199A,E200A, D201A

    Promoter

    Available since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRAPa(RKRKR232-236AAAAA)-3xFLAG-pCDNA

    Plasmid

    #201967

    Purpose

    Mammalian expression of mutated Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Use

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Mutation

    R232A, K233A, R234A, K235A, R236A

    Promoter

    Available since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRAPa(W255A,N262E,K266E)-3xFLAG-pCDNA

    Plasmid

    #201968

    Purpose

    Mammalian expression of mutated Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Use

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Mutation

    W255A,N262E,K266E

    Promoter

    Available since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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