We narrowed to 167,150 results for: Gene
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Plasmid#127199PurposePlasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoterDepositorInserttRNA-gRNA
UseEmpty sgrna plasmidExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)MBII-85-5'/3'ss mut
Plasmid#67645PurposeMBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.DepositorInsertMBII85 snoRNA
ExpressionMammalianMutation5' and 3' splice sites mutationsAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-eGFP-L3 #BGV201
Plasmid#48979PurposeSupplies the selection marker (eGFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInserteGFP
UseGateway cloning vectorTagsIRES (strong)PromoterNAAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iNeo-L3 #BGV207
Plasmid#48986PurposeSupplies the selection marker (neomycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertneomycin resistance
UseGateway cloning vectorTagsIRES (weak)PromoterNAAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-eCFP-L3 #BGV202
Plasmid#48977PurposeSupplies the selection marker (eCFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInserteCFP
UseGateway cloning vectorTagsIRES (strong)PromoterNAAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMU103
Plasmid#73934PurposeThis is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.DepositorInsertbar and GUS cassettes
ExpressionPlantPromoter35SAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-GST
Plasmid#39286DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Mutation-1163 to +6 of nmt1 locusAvailable SinceSept. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-Gm129
Plasmid#53765Purposemammalian expression of EGFP-Gm129DepositorAvailable SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
RCAS (B) Eya2 (CT#665)
Plasmid#13877DepositorAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-BmNG
Plasmid#232204PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy-mNeonGreenDepositorInsertmNeongreen
ExpressionBacterialAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-BmSc
Plasmid#232205PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy-mScarletDepositorInsertmScarlet
ExpressionBacterialAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-BTy
Plasmid#232206PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTyDepositorInsert3xTy
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-PTy
Plasmid#232203PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-PAC-3xTyDepositorInsert3xTy
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-BmSc
Plasmid#232208PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-BSD-3xTyDepositorInsertmScarlet
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-BTy
Plasmid#232209PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-BSD-3xTyDepositorInsert3xTy
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-PTy
Plasmid#232202PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTyDepositorInsert3xTy
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-PmSc
Plasmid#232548PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mScarletDepositorInsertmScarlet
ExpressionBacterialMutationNoneAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
JD179
Plasmid#229496PurposeAdenoviral helper plasmid containing inducible E2A and L4-22k/33k genes and constitutive VA RNA I geneDepositorInsertE2A, L4-22k/33k, VA RNA I
UseAAV and AdenoviralExpressionMammalianPromoterTre (E2A, L4-22k/33k). Wt promoter (VA RNA I), ET…Available SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-AGG-NLS-EGFP
Plasmid#214602Purposeexpress EGFP ; in vitro transcribed modified RNA vectorDepositorInsertEGFP
UseUnspecifiedPromoterT7Available SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-AtTAS1c-D2-NbSu-2-AtMIR173a
Plasmid#213401PurposePlant expression vector (2x35S) for expressing a syn-tasiRNA against Nicotiana benthamiana SULFUR gene from AtTAS1c precursorDepositorInsertArabidopsis TAS1c with a syn-tasiRNA sequence at D2 for silencing N. benthamiana SULFUR gene. MIR173 cassette.
ExpressionPlantPromoter2x35SAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDA2ScIfm
Plasmid#193786PurposeExpresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selectionDepositorInsertFrame-shifted CI
UseSynthetic BiologyExpressionBacterialPromoterPLTATAA synthetic promoter carrying binding sites…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTA1cI-T3
Plasmid#193789PurposeExpresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selectionDepositorInsertTruncated version of Lambda CI, named T3, where the first two codons are deleted
UseSynthetic BiologyExpressionBacterialPromoterPLTetO-1Available SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
puc19_N_tag
Plasmid#186650PurposeEmpty donor plasmid.DepositorInsertN-terminal 3xFlag-3xHA Tag
Tags3xFlag-3xHAExpressionInsectAvailable SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj13
Plasmid#173143PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj13 (kcnj13 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj1b
Plasmid#173126PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj1b (kcnj1b Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj8
Plasmid#173134PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertUsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pREG(R1-R4) #BGV059
Plasmid#48991PurposeRetroviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)DepositorTypeEmpty backboneUseRetroviralPromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
RCAS (B) Dach2 (CT#557)
Plasmid#13873DepositorInsertDach2 (DACH2 Chicken)
UseRetroviral; Avian expressionAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG Dach2 (CT#558)
Plasmid#13874DepositorAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FuG-C
Plasmid#67512PurposeThis is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type C envelope coating virus particle with NeuRet.DepositorInsertFusion Glycoprotein type C
UseLentiviralTagsFusion protein of Vesicular stomatitis Indiana vi…PromoterCAGGSAvailable SinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FuG-B
Plasmid#67523PurposeThis is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B envelope coating virus particle with HiRet by using this plasmid.DepositorInsertFusion Glycoprotein type B
UseLentiviralTagsFusion protein of Vesicular stomatitis Indiana vi…PromoterCAGGSAvailable SinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRRL.PPT.SFFV.MCS.IRES.dTomato
Plasmid#171174PurposeLentiviral expression vector co-expressing dTomatoDepositorInsertdTomato
UseLentiviralAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2-kpn1-bamH1-stop
Plasmid#20704DepositorInsert4 finger ZFP
TagsFlagExpressionBacterialAvailable SinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDS2149
Plasmid#184275PurposeTet-on genome insertion systemDepositorInsertCatrTA
ExpressionYeastPromoterTDH3Available SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pwAB-erg10erg13
Plasmid#227346PurposeOverexpression of the erg10 and erg13 genes in Aspergillus oryzaeDepositorInsertExpression cassettes of the erg10 and erg13 genes
UseSynthetic BiologyAvailable SinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYL192 (TRV1)
Plasmid#148968PurposeTobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)DepositorInsertTRV RNA1
UsePlant vector with trv rna1; this vector used with…MutationP697S mutation in putative replicase. S239G and S…Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGT8163 (AATG_ttLbCas12a-i_TTCG)
Plasmid#192453Purposeintronized ttLbCas12a for in planta expressionDepositorInserttemperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
UseCRISPR and Synthetic Biology; Moclo compatible le…Mutationtemperature-tolerant mutation (D156R)Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-5SA/S94A
Plasmid#33103DepositorAvailable SinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_NP_EBOV
Plasmid#103049PurposeEncodes Ebola virus NP gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertNP gene of Ebola virus (NP Zaire ebolavirus, Mayinga)
ExpressionMammalianMutationsilent mutation (T to C) at position 928 of NP ge…PromoterCAGAvailable SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-TEAD4
Plasmid#33105DepositorAvailable SinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only