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Addgene

We narrowed to 1,414 results for: puc57

Showing: 1051 - 1100 of 1414 results
  1. AA365

    Plasmid
    #215969
    Purpose
    Fragmid fragment: (Pol 2 promoter) Gal4-induced expression
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    LoxP_v1; 5xUAS_v1; LoxP_v1; 5xUAS_v1; Hsp70_v1
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. AA371

    Plasmid
    #215989
    Purpose
    Fragmid fragment: (N' terminus) attenuate expression
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    Start; u(S)ORF_v1.1; Stop; Start; HIV-NES_v1.2; HIV-NES_v1.1
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. AA195

    Plasmid
    #215984
    Purpose
    Fragmid fragment: (N' terminus) ALFA tag for recruiting via NbALFA
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    Start; BPNLS_v1.1; ALFA_v1.3; ALFA_v1.2; ALFA_v1.1; ALFA_v1.4; ALFA_v1.5
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. AA198

    Plasmid
    #215985
    Purpose
    Fragmid fragment: (N' terminus) activator from p65 subunit of mouse Nfkb
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    Start; BPNLS_v1.1; p65_v1.1
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. AA125

    Plasmid
    #215982
    Purpose
    Fragmid fragment: (N' terminus) activator from p65 subunit of mouse Nfkb
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    Start; p65_v1.3; HSF1_v1.2; Linker_v3.1; SV40NLS_v1.6
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. AA081

    Plasmid
    #215977
    Purpose
    Fragmid fragment: (N' terminus) HIV Nuclear Export Sequence
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    Start; HIV-NES_v1.2; HIV-NES_v1.1
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. AA124

    Plasmid
    #215981
    Purpose
    Fragmid fragment: (N' terminus) catalytic core of human acetyltransferase p300
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    Start; p300_v1.2; Linker_v3.1; SV40NLS_v1.6
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. AA122

    Plasmid
    #215980
    Purpose
    Fragmid fragment: (N' terminus) 4xVP16 transcritional activators
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    Start; VP64_v1.1; Linker_v3.1; SV40NLS_v1.6
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. AA334

    Plasmid
    #216068
    Purpose
    Fragmid fragment: (2A Selection) Stop; IRES2; Start; BlastR; Stop
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    Stop; IRES2_v1; Start; BlastR_v1.1; Stop
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. AA151

    Plasmid
    #215938
    Purpose
    Fragmid fragment: (guide cassette) for dual expression sgRNA
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; BsmBI_v0; BsmBI_v4; rev{H1_v1}
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. AA375

    Plasmid
    #216054
    Purpose
    Fragmid fragment: (C' terminus) HIV nuclear export sequence; HA tag
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    HIV-NES_v1.1; HIV-NES_v1.2; 3xHA_v1.1
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. AA428

    Plasmid
    #216055
    Purpose
    Fragmid fragment: (C' terminus) NpNLS; 3xHA; c-mycNLS; c-mycNLS
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    NpNLS_v1.1; 3xHA_v1.1; c-mycNLS_v1.2; c-mycNLS_v1.3
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  13. AA400

    Plasmid
    #215958
    Purpose
    Fragmid fragment: (guide cassette) guide expression
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; DR_v1 [Rfx]; BsmBI_v1; BsmBI_v12
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. AA401

    Plasmid
    #215959
    Purpose
    Fragmid fragment: (guide cassette) guide expression
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; DR_v1 [Dj]; BsmBI_v1; BsmBI_v12
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  15. AA370

    Plasmid
    #215988
    Purpose
    Fragmid fragment: (N' terminus) attenuate expression
    Depositor
    Has Service
    Cloning Grade DNA
    Insert
    Start; u(S)ORF_v1.1; Stop; Start; BPNLS_v1.1
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  16. kiCAP-AAV-PAL1D

    Plasmid
    #196686
    Purpose
    Rep/Cap plasmid for the production of PAL1D, an AAV capsid with CNS tropism in macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    PTQGTFR insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
  17. kiCAP-AAV-PAL1B

    Plasmid
    #196684
    Purpose
    Rep/Cap plasmid for the production of PAL1B, an AAV capsid with CNS tropism in macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    PSQGTLR insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
  18. kiCAP-AAV-PAL1A

    Plasmid
    #196683
    Purpose
    Rep/Cap plasmid for the production of PAL1A, an AAV capsid with CNS tropism in macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    PTQGTVR insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
  19. fru-T2A-QF2 HDR plasmid

    Plasmid
    #141099
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru gene
    Depositor
    Insert
    fru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
    Use
    Donor template
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
  20. pTS1043

    Plasmid
    #169621
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .
    Depositor
    Insert
    PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV / PPGK
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  21. pTS2361_Tier3(SB)-rtTA-YPet_PuroR

    Plasmid
    #169657
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  22. pTS1042

    Plasmid
    #169620
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
    Depositor
    Insert
    PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV / PPGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  23. pTS2363_Tier3(PB)-rtTA-YPet_PuroR

    Plasmid
    #169659
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
  24. FLAG-LoxP-PURO-LoxP-SNAP-TERT

    Plasmid
    #71390
    Purpose
    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.
    Depositor
    Insert
    TERT (TERT Human)
    Use
    Hr donor
    Tags
    FLAG-SNAP
    Expression
    Mutation
    Promoter
    Endogenous TERT promotor
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  25. iOn-CAG∞MCS

    Plasmid
    #154013
    Purpose
    Expression vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with an MCS to clone-in genes of interest and express it from a CAG promoter
    Depositor
    Insert
    MCS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  26. LiOn-CAG∞GFP

    Plasmid
    #154015
    Purpose
    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein EGFP from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Insert
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
  27. LiOn-CAG∞RFP

    Plasmid
    #154016
    Purpose
    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Insert
    mRFP1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    H210P
    Promoter
    CAG
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  28. iOn-CAG∞RFP

    Plasmid
    #154014
    Purpose
    Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Insert
    mRFP1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1051 - 1100 of 1414 results