Addgene

We narrowed to 12,144 results for: car

Showing: 421 - 440 of 12144 results

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pPOTv6-blast-His:mScarletI:His

Plasmid

#201097

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)

Plasmid

#191391

Purpose

Donor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locus

Depositor

Yannick Doyon

Insert

hCdt1(30-120)

Use

CRISPR and Synthetic Biology

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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TVBB N-term-mScarlet

Plasmid

#169218

Purpose

Targeting vector backbone to support a knock-in of mScarlet-Linker at the N-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mScarlet

Plasmid

#169219

Purpose

Targeting vector backbone to support a knock-in of Linker-mScarlet at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Double UP mScarlet to mNeon

Plasmid

#125139

Purpose

Natively produces mScarlet, in the presence of Cre will be recombined to produce mNeon.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)

Plasmid

#191389

Purpose

Donor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus

Depositor

Yannick Doyon

Insert

hCdt1(1-100)

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo*

Plasmid

#140159

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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Scarlet I-FRET 10-NeonGreen

Plasmid

#139364

Purpose

FRET Standard

Depositor

Richard Day

Article

Reissaus et al Biophys J. 2020 Apr 21;118(8):1820-1829.

Insert

Scarlet 10AA Neon green

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mScarlet-hCenpC

Plasmid

#171490

Purpose

T7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

CenpC (CENPC Human)

Use

Pgehme

Tags

mScarlet

Expression

Mutation

Promoter

T7

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-Mup1-mScarlet2I

Plasmid

#140152

Purpose

Plasmid for pop-in/pop-out replacement of Mup1 with Mup1-mScarlet2I

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

MUP1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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CIp10 - mScarlet-I IDT

Plasmid

#163120

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

mScarlet-I IDT

Use

Candida albicans expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR-NLS

Plasmid

#64931

Purpose

Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR-NLS (EIF4EBP1 Synthetic, Human)

Use

Tags

Cerulean, Nuclear localization signal (NLS), and …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet

Plasmid

#130509

Purpose

expresses mScarlet

Depositor

Dorus Gadella

Article

Bindels et al Nat Protoc. 2020 Feb;15(2):450-478. doi:

Insert

mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-CAR-GECO1

Plasmid

#45493

Purpose

Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

Depositor

Robert Campbell

Article

Wu et al ACS Chem Neurosci. 2013 Mar 19.

Insert

CAR-GECO1

Use

Tags

Expression

Mammalian

Mutation

R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

Promoter

CMV

Available since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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CMV mScarlet-LC3B

Plasmid

#200431

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

Map1lc3b (Map1lc3b Rat)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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3_T7-mScarlet3-KRas6R

Plasmid

#225930

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras6R membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

mScarlet3-KRas6R

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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10_T7-SP-CD8tm-mScarlet3

Plasmid

#225937

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and the CD8 transmembrane domain

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

SP-CD8tm-mScarlet3

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLBS.EF/mScarlet(i)-hCdt100Nt

Plasmid

#129334

Purpose

Cell cycle reporter, lentivirus

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

mScarlet(i) (Addgene 85044)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-C-3xVHH05-DsRed

Plasmid

#171580

Purpose

For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Hidde Ploegh, Norbert Perrimon

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3xVHH05 (UBE2J1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pN1-COX8-mScarletI

Plasmid

#129341

Purpose

Mitochondria matrix localization

Depositor

Yasushi Okada

Insert

mScarlet(i) (Addgene 85044)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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