-
Plasmid#176175Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-2DepositorInsert3' homology for integration site XII-2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-X4dw
Plasmid#176165Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-4DepositorInsert3' homology for integration site X-4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-I-Sce1-slc6a3[11kb]:egfp
Plasmid#53240Purposeallow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthoodDepositorInsertDAT-GFP
UseCloningTagsGFPExpressionMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
OpenLoopControl
Plasmid#59895Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Does not contain the target containing Vamp3 3′UTRDepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPESTExpressionMammalianMutationPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC1-PIP-SHOW
Plasmid#42212PurposeGenetically encoded fluorescent indicator for simultaneous PIP3 and hydrogen peroxide detection.DepositorInsertPIP-SHOW
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 PUM FL
Plasmid#112997PurposeFor protein expression and purification of Drosophila pumilio (amino acids 566-1090)DepositorInsertpumilio (pum Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOpen-BsupolLF
Plasmid#165563PurposeBsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.DepositorInsertBsu DNA Polymerase I, Large Fragment
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
-
FLAG-ADA add back mutant pCMV5
Plasmid#17554DepositorInsertFLAG-FoxO1-ADA
UseTagsFLAGExpressionMammalianMutationsiRNA-resistant Foxo1 by replacing 3 residues (un…PromoterAvailable sinceOct. 17, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBN095
Plasmid#173765Purposeexpress E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncationDepositorInsertE. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
UseTagshexa-histidine tagExpressionBacterialMutationdelete motif 3PromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN093
Plasmid#167951Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncationDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
UseTagshexa-histidine tagExpressionBacterialMutationK540M, delete motif 3PromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Vps34-Flag
Plasmid#24398DepositorInsertVps34 (PIK3C3 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pVETL-Cmcs-Flag-Ataxin3Q80
Plasmid#22120DepositorInsertAtaxin 3 (ATXN3 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pVETL-Cmcs-Flag-Ataxin3Q25
Plasmid#22118DepositorInsertAtaxin 3 (ATXN3 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEY51
Plasmid#191054Purposemec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertmec-3 (mec-3 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY42
Plasmid#191046Purposedhc-3 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertdhc-3 (dhc-3 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY44
Plasmid#191048Purposeflp-3 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertflp-3 (flp-3 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY37
Plasmid#191041Purposesrsx-3 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertsrsx-3 (srsx-3 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY105
Plasmid#223452Purposemgl-3 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertmgl-3 (mgl-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEY116
Plasmid#223463Purposesax-3 fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)DepositorInsertsax-3 (sax-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only