We narrowed to 531 results for: gcg.2

1. lentiSAMv2 CAMK1D TSS-guide1

   Plasmid

   #118192

   Purpose

   CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUPER IKKbeta

   Plasmid

   #26209

   Depositor

   Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   pSUPER IKK beta (IKBKB Human)
   

   Use

   RNAi

   Available Since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pPN022

   Plasmid

   #91668

   Purpose

   Express sgRNA targeting human SHANK3

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   SHANK3 (SHANK3 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. VirTREX2-HLDel-1_NbPDS

   Plasmid

   #231146

   Purpose

   T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbPDS

   Depositor

   Daniel Voytas

   Article

   Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

   Insert

   TREX2 and mobile gRNA targeting NbPDS
   

   Expression

   Plant

   Promoter

   PEBV sub-genomic

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. VirTREX2-HLDel-2_NbPDS

   Plasmid

   #231145

   Purpose

   T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbPDS

   Depositor

   Daniel Voytas

   Article

   Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

   Insert

   TREX2 and mobile gRNA targeting NbPDS
   

   Expression

   Plant

   Promoter

   PEBV sub-genomic

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCF821-sgNT-3_U6-sgRNA-EF1a-mNeonGreen

   Plasmid

   #211654

   Purpose

   U6-sgRNA-EF1a-mNeonGreen

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   SpyCas9 and sgNT-3 guide RNA vector
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPN167

   Plasmid

   #91586

   Purpose

   Express sgRNA targeting human CNTN4

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   CNTN4 (CNTN4 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPgiCas13b-gRNA-NT

   Plasmid

   #184835

   Purpose

   Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSG4K5

   Plasmid

   #74492

   Purpose

   For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

   Insert

   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPbuCas13b-gRNA-NT

    Plasmid

    #184568

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiU6-mNeurog2 gRNA_1-MS2-Puro

    Plasmid

    #192680

    Purpose

    Lentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Neurog2 activating gRNA #1 (Neurog2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pegRNA_HEK4_G-T

    Plasmid

    #201975

    Purpose

    Express a pegRNA used for G to T conversion at HEK4 site

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    pegRNA_HEK4_G-T
    

    Expression

    Mammalian

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pegRNA_HEK4_1bp-deletion

    Plasmid

    #201976

    Purpose

    Express a pegRNA used for 1bp deletion at HEK4 site

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    pegRNA_HEK4
    

    Expression

    Mammalian

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-SAG1-DHFR

    Plasmid

    #80322

    Purpose

    Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Insert

    SAG1 sgRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSIN-Ski-gRNA2

    Plasmid

    #180369

    Purpose

    targeting mouse Ski gene

    Depositor

    Dan Littman

    Article

    Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

    Insert

    Ski targeting gRNA (Ski Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. RBM15B_3xRRM_pGEX

    Plasmid

    #135125

    Purpose

    Recombinant expression of RBM15B with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    RBM15B (RBM15B Human)
    

    Tags

    GST-SBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pegRNA_HEK4_1bp-insertion

    Plasmid

    #201977

    Purpose

    Express a pegRNA used for 1bp insertion at HEK4 site

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    pegRNA_HEK4_1bp-insertion
    

    Expression

    Mammalian

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSIN-Ski-gRNA1

    Plasmid

    #180368

    Purpose

    targeting mouse Ski gene

    Depositor

    Dan Littman

    Article

    Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

    Insert

    Ski targeting gRNA (Ski Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPgiCas13b-gRNA-1

    Plasmid

    #184834

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCRISPR-v1-sgHK2_2

    Plasmid

    #163461

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting HK2

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    sgRNA 2 targeting HK2 (HK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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