We narrowed to 62,913 results for: cloning
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Plasmid#83487PurposeBacterial expression of WT L. shahii C2c2 CRISPR effectorDepositorInsertLsh_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDuRCC-N
Plasmid#81194PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease view depositor comments below and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311 ETV4-L
Plasmid#74982PurposeConstitutive ETV4 ORF, blasticidin selectionDepositorAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST47-SAR1-GFP
Plasmid#67409PurposeMammalian expression of hSar1 with C-term GFP tagDepositorAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINB-OTUB1*
Plasmid#65441PurposeExpresses activated OTUB1 in E. coliDepositorTagsHis-3CExpressionBacterialMutationActive site cysteine mutated to alaninePromoterT7Available sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFETCH_CREB1
Plasmid#64938PurposeHomology Arms and 3X Flag with P2A Neo for 3' tagging of human CREB1DepositorPublicationAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-IKKbeta (S177E,S181E)-pcw107
Plasmid#64609Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertIKBKB
UseLentiviralTagsFLAGMutationS177E, S181EPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP
Plasmid#58959Purposelentiviral expression of v-H-Ras and luciferase/EGFP reporterDepositorInsertsCMV enhancer/chicken beta actin promoter
v-H-Ras
luc2/EGFP fusion gene
UseLentiviralExpressionMammalianPromoterCMV enhancer/chicken beta actinAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOTTC293 - pAAV EF1a V5-synuclein (WT)
Plasmid#60057PurposeAn AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.DepositorAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLSL
Plasmid#51493PurposeT-DNA destination plasmid. Contains the bean yellow dwarf virus cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. Contains attR1 and attR2 sites between the LIR and SIR sequence.DepositorTypeEmpty backboneUsePlant t-dna plasmidPromotervirion-sense LIR and 2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-mCherry-GJA1-20k-N1
Plasmid#49861PurposeEncodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tagDepositorInsertGJA1-20k (GJA1 Human)
TagsmCherryExpressionMammalianMutationGJA1-20k corresponds to residues 213 to 382 to Co…PromoterCMVAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLIK 3xHA-MKK6-EE neo
Plasmid#47546PurposeLentiviral expression vector for an inducible 3xHA-tagged constitutively active MKK6 mutantDepositorAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pECIA2
Plasmid#47032PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.DepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsFc(human IgG1), HRV 3C Protease Cleavage site, He…ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available sinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
PGAM5
Plasmid#38976PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-N EBNA3A
Plasmid#37956DepositorInsertEBNA3A (EBNA-3A H. Herpesvirus 4 (EBV))
UseRetroviralTagsFlag and HAExpressionMammalianPromoterPKGAvailable sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pEntr-Tbx5flbio
Plasmid#32968DepositorAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAd/CMV/DAX1-IRES-nEGFP
Plasmid#29752DepositorInsertdosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (NR0B1 Human)
TagsEGFP, IRES, and Nuclear localization sequenceExpressionMammalianMutationNo mutations; Reporter is nuclear localized EGFP;…Available sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
458 p5E-uasE1b
Plasmid#26026DepositorInsertE1B basal promoter + UAS repeats
UseGateway CloningAvailable sinceAug. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SPHK2
Plasmid#23714DepositorInsertSPHK2 (SPHK2 Human)
UseGateway CloningAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by