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We narrowed to 63,680 results for: cloning

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Showing: 4381 - 4400 of 63680 results

  5. pUCmini-iCAP-AAV9.452sub.LUNG1

    Plasmid
    #184592
    Purpose
    non-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system for mouse lung transduction after intravenous injection
    Depositor
    Viviana Gradinaru
    Publication
    Goertsen et al Mol Ther Methods Clin Dev. 2022 Aug 1;26
    Insert
    Synthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
    Use
    AAV
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    July 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    184592-plasmid-map-sequence-id-364433

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  9. pCMV-CD63-L7Ae

    Plasmid
    #180503
    Purpose
    Human CD63-L7Ae fusion protein
    Depositor
    Masaharu Somiya
    Publication
    Somiya et al bioRxiv 2022.01.25.477620
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human CD63-L7Ae fusion protein
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    180503-plasmid-map-sequence-id-357065

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  10. pX458-Dual-Guide-Donor-ShuttleOnly

    Plasmid
    #171098
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Publication
    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. GtoC mu…
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    171098-plasmid-map-sequence-id-336112

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  12. PGK plasmid

    Plasmid
    #169744
    Purpose
    Unregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.
    Depositor
    Francois St-Pierre
    Publication
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    PGK promoter
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169744-plasmid-map-sequence-id-334452

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  13. SpyCas9 PE2*

    Plasmid
    #169850
    Purpose
    Mammalian Expression, SpyCas9 prime editor, optimized NLS
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9 PE*
    Tags
    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    June 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169850-plasmid-map-sequence-id-332905

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  14. pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX

    Plasmid
    #167583
    Purpose
    Express the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cells
    Depositor
    Yulong Li
    Publication
    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    167583-plasmid-map-sequence-id-330893

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  16. pUCM-CLYBL-NGN2-BRN3A

    Plasmid
    #165597
    Purpose
    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.
    Depositor
    Carsten Bönnemann
    Publication
    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Promoter
    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…
    Available since
    March 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165597-plasmid-map-sequence-id-326012

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  18. pExp-GB1

    Plasmid
    #112565
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-GB1 and Strep tag
    Expression
    Bacterial
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112565-plasmid-map-sequence-id-217228

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Showing: 4381 - 4400 of 63680 results