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pDONR-221-P3-P4

Plasmid

#186351

Purpose

Gateway donor vector with AttP3 AttP4 recombination sites

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS_cAMPFIRE-L-R279E

Plasmid

#182286

Purpose

Mammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-nLight3.1

Plasmid

#111061

Purpose

Expresses nLight3.1 in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

nLight3.1

Tags

Flag tag

Expression

Mammalian

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-4xnrUAS

Plasmid

#87120

Purpose

Multisite gateway entry clone containing 4 non-repetitive UAS (upstream activation sequence) elements

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

4xnrUAS (non repetitive)

Use

Gateway vector

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.5

Plasmid

#111058

Purpose

Expresses dLight1.5 in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.5

Tags

Flag tag

Expression

Mammalian

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pUBC-paGFP-Dest

Plasmid

#105107

Purpose

fluorescent tagging in stably transformed plants

Depositor

Christopher Grefen

Article

Blatt et al Methods Mol Biol. 2014;1062:487-507. doi

Type

Empty backbone

Tags

paGFP

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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p5E-ESyn1

Plasmid

#80804

Purpose

hSyn1 promoter fused to CMVmin

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p5E-ESyn1

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCM291rfp

Plasmid

#59775

Purpose

pCMrfp with TrfA mutation (291E to G)

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Expression

Bacterial

Promoter

pBAD

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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RCASBP (E) (CC#36)

Plasmid

#15163

Depositor

Connie Cepko

Article

Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

Type

Empty backbone

Use

Retroviral; Avian expression

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pBR322-Pdp1_NTD-ZFD_L

Plasmid

#198276

Purpose

For loading L half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Left-G1397-N_DddA); also contains PVC regulatory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-ZFD_L

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBL6

Plasmid

#50549

Purpose

Expresses mCherry under the PLtetO-1 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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B-gTEMP/pRSET-b

Plasmid

#188446

Purpose

B-gTEMP for bacterial expression

Depositor

Takeharu Nagai

Article

Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

Insert

B-gTEMP

Tags

6xHis-tag

Expression

Bacterial

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCTCON2-Aga1p

Plasmid

#158141

Purpose

Expresses Aga1p in yeast

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p

Expression

Yeast

Promoter

GAL10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-sfGFP-24xGCN4_v1

Plasmid

#61056

Purpose

A direct fusion of sfGFP to the 24xGCN4_v1 peptide array

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

sfGFP-24xGCN4_v1 peptide array

Expression

Mammalian

Available Since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only
pCfB8644

Plasmid

#126919

Purpose

Plasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and HMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMDICI

Plasmid

#51652

Purpose

E coli genetic modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

CmR

Use

Genetic modification

Promoter

unknown

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only
pBabe-TetCMV-puro-mPA-GFP-PA-Rac1

Plasmid

#22032

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mPA-GFP-PA-Rac1 (RAC1 Avena sativa (oat), Human)

Use

Retroviral

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pJR107

Plasmid

#187245

Purpose

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter

Use

Lentiviral

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJR106

Plasmid

#187244

Purpose

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter

Use

Lentiviral

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pN1-QUE37C

Plasmid

#129318

Purpose

ATP measurement

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Expression

Mammalian

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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