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pLKO-puro-sh-mFAK C

Plasmid

#37015

Depositor

Bob Weinberg

Article

Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

Insert

sh-mFAK C (Ptk2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
pDEST53-LRRK2-3XKD-R1441C

Plasmid

#25050

Depositor

Mark Cookson

Article

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
FUS_anion

Plasmid

#98656

Purpose

Expresses untagged FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

Insert

FUS (FUS Human)

Expression

Bacterial

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pKHH030

Plasmid

#89358

Purpose

hU6 sgRNA cloning vector for CDKO system

Depositor

Michael Bassik

Article

Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

Insert

GFP, Puromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 13, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-UBE2S-UBD

Plasmid

#66713

Purpose

Expresses the UBE2S-UBD fusion protein in E. coli

Depositor

David Komander

Article

Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

Inserts

e2-epf (UBE2S )
isot (USP5 )

Tags

GST-3C

Expression

Bacterial

Promoter

T7

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pCEP4-HA-MEKK1-1-1493

Plasmid

#21632

Depositor

Melanie Cobb

Article

Xu et al Proc Natl Acad Sci U S A. 1995 Jul 18. 9

Insert

mitogen-activated protein kinase kinase kinase 1 (Map3k1 Rat)

Tags

HA

Expression

Mammalian

Available Since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only
pBMN-2xFKBP-mEGFP-NDP52

Plasmid

#110465

Purpose

retrovirus construct for stable expressing mEGFP tagged NDP52

Depositor

Richard Youle

Article

Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

Insert

NDP52 (CALCOCO2 Human)

Use

Retroviral; Retrovirus

Tags

mEGFP

Expression

Mammalian

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pOZ-N-FH KIAA0157

Plasmid

#27499

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

KIAA0157 (ABRAXAS2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA_DiCre_59.F2

Plasmid

#60207

Purpose

Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Synthetic, Bacteriophage P1, Human)

Tags

FKBP12 and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 19-59 of the humanized version of Cre…

Promoter

CMV

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAP3K8

Plasmid

#23538

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K8 (MAP3K8 Human)

Use

Gateway Cloning

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

Plasmid

#216153

Purpose

Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with cryptic splice site and C-terminal FLAG tag

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_3xFLAG_V5_ mmDdx58_V5

Plasmid

#160108

Purpose

Expresses murine Ddx58 in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Ddx58 (Rigi Mouse)

Use

Lentiviral

Tags

3xFLAG, V5

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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CAG-NeuroD6iresGFP

Plasmid

#45027

Depositor

Connie Cepko

Article

Cherry et al J Neurosci. 2011 May 18;31(20):7365-79.

Insert

NeuroD6 (Neurod6 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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pOZ-N-FH MERIT40

Plasmid

#27498

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

MERIT40 (BABAM1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag ADRBK2

Plasmid

#20419

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

ADRBK2 (GRK3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CERK

Plasmid

#20454

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CERK (CERK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
XE120 CAMKII-K42M 1-271-CS2+

Plasmid

#16734

Depositor

Randall Moon

Article

Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

Insert

CAMKII-K42M 1-271 (Camk2a Rat)

Use

Xenopus expression

Mutation

C-terminal truncation of K42M kinase

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO/Strep-HA-ZAKalpha_K45A

Plasmid

#141194

Purpose

Expresses kinase-dead Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 (MAP3K20 Human)

Tags

Strep, HA

Expression

Mammalian

Mutation

Changed Lysine 45 to Alanine

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNATM3.1/V5-His TOPO-PKCi-K274W

Plasmid

#55690

Purpose

expresses dominant negative human PKCi tagged with V5

Depositor

Pedro Salas

Article

Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

Insert

Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)

Tags

V5/His

Expression

Mammalian

Mutation

A120E and K274W

Promoter

CMV

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only
pAF0034 Ef1a-Kp03-2a-EGFP

Plasmid

#193461

Purpose

Expression of Kp03 recombinase from Ef1a promoter

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Kp03

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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