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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 40,052 results for: lat

Showing: 4381 - 4400 of 40052 results

  3. ITGB3

    Plasmid
    #51921
    Purpose
    Expresses full-length Integrin beta-3 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ITGB3 (ITGB3 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 718, before CD4, bio and…
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51921-plasmid-map-sequence-id-79900

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  5. pBiFC-PBZ-4A-VC

    Plasmid
    #110649
    Purpose
    expresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR binding
    Depositor
    Chris Lord
    Article
    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:
    Insert
    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    Tags
    HA and VC
    Expression
    Mammalian
    Mutation
    C379A, C385A, C421A and C427A
    Promoter
    CMV
    Available Since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110649-plasmid-map-sequence-id-217742

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  7. pBrain-ch-TOGKDP-GFP-shch-TOG

    Plasmid
    #69113
    Purpose
    Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
    shch-TOG
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Silent mutations to confer shRNA resistance.
    Promoter
    CMV
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    69113-plasmid-map-sequence-id-121841

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  8. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid
    #99277
    Purpose
    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mOrange2-IRES-EGFP
    Puro
    Use
    Lentiviral
    Tags
    EGFP, H2B, IRES, PGK, and mOrange2
    Promoter
    EF1 alpha-human and PGK-mouse
    Available Since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99277-plasmid-map-sequence-id-197594

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  10. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5

    Plasmid
    #97044
    Purpose
    Expresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.
    Depositor
    George Daley
    Article
    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature
    Insert
    Ligand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Tet on
    Available Since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    97044-plasmid-map-sequence-id-192694

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  11. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

    Plasmid
    #196185
    Purpose
    Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755
    Inserts
    GSK3B (GSK3B Human)
    λPPase
    Tags
    GST, HA, His6, and MBP
    Expression
    Bacterial
    Mutation
    changed Serine 9 to Alanine
    Promoter
    Tac
    Available Since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196185-plasmid-map-sequence-id-383261

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  12. pICE-HA-Ku70-siR-Mut6E

    Plasmid
    #82330
    Purpose
    Plasmid for constitutive or doxy-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10
    Insert
    Ku70 (XRCC6 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Mut6E corresponding to K282E K287E T300E K331E K3…
    Promoter
    CMV-tet
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    82330-plasmid-map-sequence-id-155730

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  16. APP-bio-His

    Plasmid
    #51643
    Purpose
    Expresses full-length Amyloid beta A4 protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    APP (APP Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51643-plasmid-map-sequence-id-78965

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  20. pACEBac1-PARP1-Flag-His-7xKQ

    Plasmid
    #111573
    Purpose
    Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells
    Depositor
    Thomas Muir
    Article
    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038
    Insert
    PARP1 (PARP1 Human)
    Tags
    C-terminal 6xHis and C-terminal Flag
    Expression
    Insect
    Mutation
    lysine to glutamine mutation at the following ami…
    Promoter
    polyhedrin promoter
    Available Since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111573-plasmid-map-sequence-id-215588

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Showing: 4381 - 4400 of 40052 results