We narrowed to 18,467 results for: Met

1. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

   Plasmid

   #104571

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
   

   Use

   CRISPR

   Tags

   NLS-3xHA-3xFLAG-4xDmrA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CaMKII-Chronos-GFP

   Plasmid

   #58805

   Purpose

   AAV expression of Chronos-GFP under the CaMKII promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CaMKII

   Available Since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMOS001: CMV-Optopatch2_FCK

   Plasmid

   #62984

   Purpose

   Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology under CMV promoter

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   Optopatch2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Tag4A-MMP8 Wt

   Plasmid

   #29545

   Depositor

   Yardena Samuels

   Article

   Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

   Insert

   Metalloproteinase protein 8 (MMP8 Human)
   

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Available Since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

5. Str-STIM1-NN_neomycin

   Plasmid

   #65311

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   STIM1 mutated for binding motif to microtubule (STIM1 Human)
   

   Tags

   streptavidin

   Expression

   Mammalian

   Mutation

   2 amino acids of STIM1 (IP from SxIP motif) were …

   Promoter

   CMV

   Available Since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)

   Plasmid

   #182395

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusions

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-LZ-VenusN154
   VNp-LZ-VenusC155
   

   Tags

   Vesicle Nucleating peptide (VNp) & Leucine Zi…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. DRH229: FCK-QuasAr1-mO2

   Plasmid

   #51629

   Purpose

   Fluorescent reporter for membrane voltage with improved brightness

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   QuasAr1-mOrange
   

   Use

   Lentiviral

   Tags

   mOrange2

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   June 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

   Plasmid

   #35509

   Purpose

   AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Has Service

   AAV5 and AAV9

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   Ef1a

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. mRuby2-MAPTau-C-10

   Plasmid

   #55904

   Purpose

   Localization: Microtubules, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   MAPTau (MAPT Human)
   

   Tags

   mRuby2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 12, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pCOTS-pyl-GFP(35TAG)

    Plasmid

    #92047

    Purpose

    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.

    Depositor

    Lital Alfonta

    Article

    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

    Inserts

    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    

    Use

    Replicative expression plasmid for cyanobacteria …

    Tags

    Histag

    Mutation

    Tyrosine in position 35 of the GFP was mutated f…

    Promoter

    LeuP (native S. elongatus promoter), PpsbII, and …

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn Con/Foff EYFP

    Plasmid

    #55651

    Purpose

    Cre-on/Flp-off EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    eYFP with introns
    

    Use

    AAV; Cre on/flp off eyfp

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. DRH322: AAV-CaMKIIa-QuasAr2-mO2

    Plasmid

    #51696

    Purpose

    AAV production of QuasAr2 fluorescent reporter

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    QuasAr2-mOrange
    

    Use

    AAV

    Tags

    mOrange2

    Promoter

    CaMKIIa

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-VTKS4

    Plasmid

    #170855

    Purpose

    AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. mRuby2-Farnesyl-5

    Plasmid

    #55896

    Purpose

    Localization: Membrane, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pTetON-DeAct-SpvB

    Plasmid

    #89455

    Purpose

    Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    DeAct-SpvB (spvB Salmonella enterica)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncation spanning SpvB amino acids 375-591 (mon…

    Promoter

    TRE3G

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAWG-dCas9-VPR

    Plasmid

    #63802

    Purpose

    SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    SP-dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Insect

    Promoter

    act5c

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

17. AAV-hSyn-REX-GECO1

    Plasmid

    #61248

    Purpose

    Expresses REX-GECO1 in neurons

    Depositor

    Robert Campbell

    Article

    Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

    Insert

    REX-GECO1
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Substitutions relative to R-GECO1: P60R, V61W, R6…

    Promoter

    hSyn

    Available Since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDF-Az

    Plasmid

    #197101

    Purpose

    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine

    Depositor

    Kensaku Sakamoto

    Article

    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

    Insert

    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    

    Expression

    Bacterial

    Mutation

    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-FLEX-rc[Chronos-GFP]

    Plasmid

    #59056

    Purpose

    Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-VTKS5

    Plasmid

    #170856

    Purpose

    AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of any transgene in neurons under the control of the hSyn1 promoter.

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge