161,570 results

1. AAV-cTNT-Cre

   Plasmid

   #209785

   Purpose

   Expresses Cre by the specific cTNT promoter

   Depositor

   Yuxuan Guo

   Article

   Yang et al Circulation. 2024 May 28;149(22):1778-17

   Insert

   Cre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   cTNT

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

   Plasmid

   #185911

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. TCARZ-CMV-Cherry-PAmin-GF3-R4A22

   Plasmid

   #203770

   Purpose

   For production of template RNA for use in PRINT, mCherry under CMV promoter

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   PRINT template RNA for mCherry
   

   Use

   Ivt template

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-puro-shFAM134B

   Plasmid

   #109013

   Purpose

   short-hairpin knockdown of targeted gene

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   shFAM134B (RETREG1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti UBC LysoFlux (HaloTag7-mGFP-Galectin3)

   Plasmid

   #215008

   Purpose

   Express a Lysophagy reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAVS1-Puro CAG FUCCI

   Plasmid

   #136934

   Purpose

   donor plasmid for constitutive FUCCI expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

   Insert

   Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
   

   Use

   Synthetic Biology

   Tags

   Clover and mKO2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PH-Akt-GFP

   Plasmid

   #51465

   Purpose

   Is a biosensor for PIP3/PI(3,4)P2

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   AKT (aa 1–164)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJR104

   Plasmid

   #187243

   Purpose

   Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mito-V5-APEX2

   Plasmid

   #72480

   Purpose

   Expresses APEX2 in mitochondria

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. TurboID-His6_pET21a

    Plasmid

    #107177

    Purpose

    expresses His6-tagged TurboID in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCX138

    Plasmid

    #229213

    Purpose

    CRISPR array plasmid for NOTCH2 (Spacers 25-48)

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    CRISPR array plasmid for NOTCH2 (Spacers 25-48)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. FKBP:FRB-FBAR

    Plasmid

    #223620

    Purpose

    Transient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    FBP17 (FNBP1 Human)
    

    Use

    Tags

    FKBP, TagBFP and mGFP, FRB

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. 0_T7-mScarlet3

    Plasmid

    #225927

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cytoplasmic PancACe

    Plasmid

    #215708

    Purpose

    Expresses acetyl-CoA biosensor in the cytoplasm

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and cytoplasmic localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti MS2-P65-HSF1_Hygro

    Plasmid

    #61426

    Purpose

    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Insert

    MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1A

    Available since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAV0286

    Plasmid

    #149333

    Purpose

    Bacterial expression of SENP_EuB protease

    Depositor

    Dirk Görlich

    Article

    Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

    Insert

    SENP_EuB protease
    

    Use

    Tags

    His14 and Tev protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MS2_adRNA-MCP_ADAR2 DD (E488Q)_NES

    Plasmid

    #124707

    Purpose

    Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NES

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    MCP_ADAR2 DD (E488Q)_NES
    

    Use

    AAV

    Tags

    NES

    Expression

    Mammalian

    Mutation

    E488Q hyperactive mutant

    Promoter

    CMV

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEM415-ldhL-mRFP1

    Plasmid

    #99842

    Purpose

    Expression of RFP in Lactobacillus

    Depositor

    Sujin Bao

    Article

    Bao et al PLoS One. 2013;8(3):e60007. doi: 10.1371

    Insert

    monomeric red fluorescent protein
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    ldhl

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-N1 human cofilin S3E

    Plasmid

    #50861

    Purpose

    expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 S3E (CFL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S3E psuedo-phosporylated, non-activatable

    Promoter

    CMV

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAM5409

    Plasmid

    #132662

    Purpose

    RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Inserts

    aadA
    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PaadA and PconII

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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