164,577 results

1. opto-Cdc42

   Plasmid

   #174510

   Purpose

   Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   opto-Cdc42-mCherry
   

   Use

   Tags

   3xFLAG and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. opto-E-cad GFP

   Plasmid

   #203327

   Purpose

   Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

   Depositor

   Seraphine Wegner

   Article

   Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

   Insert

   Photoswitchable - E-cadherin with GFP tag (CDH1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Addition of Aslov2 domain at T133 of human E-cadh…

   Promoter

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-hATG7wt

   Plasmid

   #87867

   Purpose

   Express a wild type human Atg7/Apg7 in mammalian cells

   Depositor

   Isei Tanida, Eiki Kominami

   Article

   Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

   Insert

   ATG7 (ATG7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIS1-Eef25UTR-renilla

   Plasmid

   #38235

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Eef2 5'UTR / Renilla (Eef2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Eef2 5' UTR fused to renilla ORF

   Promoter

   Endogenous Eef2

   Available Since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

   Plasmid

   #172982

   Purpose

   control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-tdTomato-CNA35

   Plasmid

   #61606

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   tdTomato and CNA35
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-GFP-Syntaxin1A

   Plasmid

   #170871

   Purpose

   Mammalian expression of His-GFP-Syntaxin1A.

   Depositor

   Justin Taraska

   Article

   Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

   Insert

   Syntaxin 1A (Stx1a Mouse)
   

   Use

   Tags

   (His)6 and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mAzurite-C1

   Plasmid

   #54583

   Purpose

   Localization: C1 Cloning Vector, Excitation: 384, Emission: 450

   Depositor

   Patrick Daugherty, Michael Davidson

   Article

   Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

   Type

   Empty backbone

   Use

   Tags

   mAzurite

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pEGFP-N2-COL1A1

   Plasmid

   #66602

   Purpose

   Expresses procollagen 1 alpha 1 as a GFP fusion

   Depositor

   David Stephens

   Article

   Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

   Insert

   COL1A1 (COL1A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. siRNA-resistant SigmaR1-mNeonGreen a4mut (I178A L182A L186A V190A)

    Plasmid

    #226577

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    I178A, L182A, L186A, V190A

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. siRNA-resistant SigmaR1-mNeonGreen a5mut (F196A L199A F200A L203A Y206A L210A L214A Y217A)

    Plasmid

    #226578

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    F196A, L199A, F200A, L203A, Y206A, L210A, L214A, …

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX208 CMV sTurboID (C)-HA-FRB-ERM

    Plasmid

    #153007

    Purpose

    Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID (C) (G74)-ERM (Cb5)
    

    Use

    Lentiviral

    Tags

    FRB and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. plenti-CAG-gate-FLAG-IRES-GFP

    Plasmid

    #107398

    Purpose

    Lentiviral gateway destination vector

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

    Plasmid

    #138130

    Purpose

    Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mCherry-anti-mouse-IgG(kappa)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2/BH

    Plasmid

    #26556

    Depositor

    Perry Hackett

    Insert

    pT2/BH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-p300

    Plasmid

    #23252

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHCKan-yibDp-mCherry

    Plasmid

    #127058

    Purpose

    Low phosphate inducible mCherry with N-terminal linker and His tag

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-C1-AR

    Plasmid

    #28235

    Purpose

    Mammalian expression of Androgen receptor fused to EGFP

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    Androgen receptor (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209722

    Purpose

    Expresses mitochondrially targeted low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-Wnt7B

    Plasmid

    #35915

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt7B (Wnt7b Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge