We narrowed to 10,893 results for: ENA;

1. pLKO-puro-tetON-shGFP

   Plasmid

   #110939

   Purpose

   Tet-inducible shRNA targeting GFP

   Depositor

   William Hahn

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

   Insert

   shGFP
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   H1/TO

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG MCP-VP64

   Plasmid

   #92357

   Purpose

   CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   MCP-VP64
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. DCT.VV.Orf45

   Plasmid

   #12733

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jon Yewdell, Jack Bennink

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf45
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

4. mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax

   Plasmid

   #173874

   Purpose

   Mammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro

   Plasmid

   #211687

   Purpose

   U6-sgRNA-EFS-Cas9-P2A-Puro

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   SpyCas9 and guide RNA recipient vector
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

   Plasmid

   #136382

   Purpose

   CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b (D570A)-TV-T2A-MCP-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D570A

   Promoter

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST

   Plasmid

   #127314

   Purpose

   Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FDIO-COX4-dAPEX2

   Plasmid

   #117178

   Purpose

   Plasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   COX4-dAPEX2
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CYFIP2.mCherry

   Plasmid

   #122052

   Purpose

   Expresses human CYFIP2 in mammalian cells.

   Depositor

   Josef Kittler

   Article

   Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

   Insert

   CYFIP2 (CYFIP2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV_pSyn_Mito-LOV-Turbo-V5

    Plasmid

    #199669

    Purpose

    expresses Mito-LOV-Turbo-V5 in neurons, AAV vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    AAV

    Tags

    COX4I1 mito targeting sequence and V5

    Expression

    Mutation

    Promoter

    Synapsin

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV_tKiMBI-T2A-caMEK

    Plasmid

    #199578

    Purpose

    Expresses tKiMBI and caMEK in an AAV vector

    Depositor

    Michael Lin

    Article

    Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

    Inserts

    ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
    constitutively active MEK
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSF-G1(6CNW)RS

    Plasmid

    #222873

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. MDH1-miR-31-PGK-GFP-Blast

    Plasmid

    #31515

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-31 (Mir31 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

14. Empty Target Control

    Plasmid

    #127667

    Purpose

    Encodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMOD_B2312

    Plasmid

    #91070

    Purpose

    Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    PvUbi1

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEY26

    Plasmid

    #191031

    Purpose

    cho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    cho-1(3.3-2.6k) (cho-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEY39

    Plasmid

    #191043

    Purpose

    acr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    acr-2(2.1k) (acr-2 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

    Plasmid

    #216292

    Purpose

    Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras heavy chain-GSG-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin

    Plasmid

    #199585

    Purpose

    Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vector

    Depositor

    Michael Lin

    Article

    Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

    Inserts

    ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
    AkaLuc
    Blasticidin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXs_FLAG-GGC1

    Plasmid

    #133258

    Purpose

    Express FLAG-tagged GGC1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    GGC1 (GGC1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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