We narrowed to 24,612 results for: Nes

1. pFBP_2xNbLYS1::LUZ

   Plasmid

   #189918

   Purpose

   Binary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.

   Depositor

   Adam Steinbrenner

   Article

   Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(

   Insert

   pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Various

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNH605-PACE-YFP

   Plasmid

   #172002

   Purpose

   Fluorescent reporter for Rpn4 activity

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   4xPACE-YFP
   

   Use

   Vector for genomic integration into the yeast gen…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CYC1 core promotor with 4 tandem repeats of Rpn4 …

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pF709-pET28a-Hs-FL-ETFbeta-NHis wt

   Plasmid

   #85110

   Purpose

   expression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human full-length ETFbeta (ETFB Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN_TT 3xFLAG-KPNB1

   Plasmid

   #192301

   Purpose

   Gateway entry vector for an inducible 3xFLAG-KPNB1

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   KPNB1 (KPNB1 Human)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FAK2_HUMAN_D0

   Plasmid

   #79747

   Purpose

   This plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   FAK2 (PTK2B Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. mCardinal-H2B-C-10

   Plasmid

   #56162

   Purpose

   Localization: Nucleus/Histones, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pBabe HA-∆Raf1(S642A):ER* puro

   Plasmid

   #72572

   Purpose

   Expresses hormone-responsive, feedback-resistant Raf allele with an N-terminal HA tag.

   Depositor

   Kevin Janes

   Article

   Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

   Insert

   Raf1 (RAF1 Human)
   

   Use

   Retroviral

   Tags

   ER* and HA

   Expression

   Mammalian

   Mutation

   amino acids 306-648, serine 642 mutated to alanine

   Promoter

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-FLEX-SaCas9-U6-sgGrin2a

   Plasmid

   #124850

   Purpose

   Mutagenesis of Grin2a

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin2a (Grin2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6

   Plasmid

   #124860

   Purpose

   Mutagenesis of Slc17a6

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a6 (Slc17a6 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5 FRT/TO/STRP 3x FLAG cyclin B1 L45A (1391)

    Plasmid

    #61841

    Purpose

    mammalian expression of cyclin B1 non-degradable L45A mutant

    Depositor

    Jonathon Pines

    Article

    Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

    Insert

    cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    2xSTREP and 3xFLAG

    Expression

    Mammalian

    Mutation

    L45A

    Promoter

    CMV

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pSLIK S6K (E389-deltaCT) neo

    Plasmid

    #58516

    Purpose

    Lentiviral expression vector for an inducible HA-tagged rat S6K1-E389-deltaCT

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Rps6kb1 (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T389E, stop codon at amino acid 402

    Promoter

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

12. KIF5C(1-560)-2xmCitrine-GFP11

    Plasmid

    #61667

    Purpose

    Tandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. BPK2797

    Plasmid

    #72251

    Purpose

    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335Q, and T1337R

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNH605-CCAAT-YFP

    Plasmid

    #172132

    Purpose

    Fluorescent reporter for HAP complex activity

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    4xCCAAT-YFP
    

    Use

    Vector for genomic integration into the yeast gen…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CYC1 core promotor with 4 tandem repeats of the H…

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. STK24_HUMAN_D0

    Plasmid

    #79742

    Purpose

    This plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Markus Seeliger, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    STK24 (STK24 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pLKO.1 shJUND puro

    Plasmid

    #58698

    Purpose

    Lentiviral shRNA vector for knockdown of human JUND

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    shJUND (JUND Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pET28b-BE3∆UGI

    Plasmid

    #96943

    Purpose

    The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).

    Depositor

    Daesik Kim

    Article

    Kim et al Nat Biotechnol. 2017 May;35(5):475-480.

    Insert

    BE3∆UGI
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEN_TT 3xFLAG-KPNA2

    Plasmid

    #192306

    Purpose

    Gateway entry vector for an inducible 3xFLAG-KPNA2

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KPNA2 (KPNA2 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)

    Plasmid

    #205098

    Purpose

    Cre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Bjorefeldt et al bioRxiv. 2023 Dec 4:2023.06.26.545546. d

    Insert

    mNeonGreen, eKL9h, and Volvox channelrhodopsin-1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. tet-pLKO shMKP1.v1 puro

    Plasmid

    #87790

    Purpose

    Expresses an inducible short hairpin targeting human MKP1 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP1 (DUSP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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