We narrowed to 11,735 results for: pcdna

1. pCDNA-myc-HisA-Sirt3

   Plasmid

   #24924

   Depositor

   Toren Finkel

   Article

   Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

   Insert

   Sirt3 (SIRT3 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pCDNA3-HA-Akt1-aa120-433

   Plasmid

   #73411

   Purpose

   Expresses HA tagged aa120-433 AKT1

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Expresses aa 120-433 of AKT1

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-Lyn-RAB-EKARev

   Plasmid

   #118477

   Purpose

   ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   Lyn-RAB-EKARev
   

   Use

   Tags

   N-terminal targeting sequence from Lyn-kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1+ GEMIN6 myc HIS

   Plasmid

   #71659

   Purpose

   Mammalian expression of GEMIN6

   Depositor

   Gideon Dreyfuss

   Insert

   GEMIN6 (GEMIN6 Human)
   

   Use

   Tags

   HIS and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA5/FRT/TO-GAlphaoB-RLuc8

   Plasmid

   #140977

   Purpose

   Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaoB-Rluc8 (GNAO1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FLAG-OTUB1 T134R-pcDNA3.1

   Plasmid

   #118211

   Purpose

   Expresses FLAG-OTUB1 T134R mutant in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   OTUB1 (OTUB1 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   changed threonine 134 to arginine

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1479 pcDNA3 flag HA 14-3-3 gamma

   Plasmid

   #9000

   Depositor

   William Sellers

   Insert

   14-3-3 gamma (Ywhag Rat)
   

   Use

   Tags

   HA and flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-MTS-CA-c-Src-FLAG

   Plasmid

   #44654

   Depositor

   Yoshimi Homma

   Article

   Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

   Insert

   v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
   

   Use

   Tags

   FLAG tag and mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   changed Tyr 530 to Phe

   Promoter

   CMV promoter

   Available since

   May 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-AU1-mTOR-L1460P

   Plasmid

   #19995

   Depositor

   Fuyuhiko Tamanoi

   Article

   Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   mammalian target of rapamycin-L1460P (Mtor Rat)
   

   Use

   Tags

   AU1

   Expression

   Mammalian

   Mutation

   Changed Leucine 1460 to Proline

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1-STIM1-Venus-173-C

    Plasmid

    #87619

    Purpose

    Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    STIM1-Venus173-C (STIM1 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-FRT/TO-FLAG-SHLD1

    Plasmid

    #114115

    Purpose

    Expresses N-terminally tagged FLAG-SHLD1 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD1 (SHLD1 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pcDNA3-Lyso-INPP4B-mCherry

    Plasmid

    #184055

    Purpose

    Lysosome targeted inositol polyphosphate-4-phosphatase type II B; tagged with mCherry.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    LGP120 Lyso-INPP4B-mCherry (LAMP1, INPP4B Human)
    

    Use

    Tags

    Lysosome-associated membrane protein 1 (LAMP1). a…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Yellow Cameleon-Nano50/pcDNA3

    Plasmid

    #51964

    Purpose

    mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano50
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA4/TO-myc- GFP-Flag

    Plasmid

    #82471

    Purpose

    Expresses myc-GFP-Flag

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    Myc-GFP-Flag
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3 c-SRC (250-536) HA

    Plasmid

    #42209

    Depositor

    Robert Lefkowitz

    Article

    Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

    Insert

    c-SRC (250-536) HA (SRC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    contains amino acids 250-536

    Promoter

    CMV

    Available since

    Jan. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCDNA3-deltaBamHI-human MIZ1

    Plasmid

    #74167

    Purpose

    Mammalian expression of human MIZ1

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    MIZ1 (ZBTB17 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5FRT PUR mGSK3A K148A

    Plasmid

    #35158

    Depositor

    Jim Woodgett

    Article

    Doble et al Dev Cell. 2007 Jun;12(6):957-71.

    Insert

    GSK-3α (Gsk3a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K148A (dominant negative)

    Promoter

    CMV

    Available since

    May 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps

    Plasmid

    #181956

    Purpose

    Genetically encoded FRET-based cAMP indicator fused to AKAP79.

    Depositor

    Jin Zhang

    Article

    Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

    Insert

    AKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)
    

    Use

    Tags

    AKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Blue opsin mcherry ---- pcDNA3.1

    Plasmid

    #62932

    Purpose

    Blue opsin tagged with mcherry on the C terminus

    Depositor

    Narasimhan Gautam

    Article

    Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    blue opsin
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1553 pcDNA3 HA gamma adaptin 2

    Plasmid

    #10713

    Depositor

    William Sellers

    Insert

    Gamma adaptin 2 (AP1G2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only