We narrowed to 10,056 results for: transfer

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pZac2.1 gfaABC1D-EGFP-Kir4.1

Plasmid

#52874

Purpose

Express EGFP-Kir4.1 fusion protein in astrocytes

Depositor

Baljit Khakh

Article

Tong et al Nat Neurosci. 2014 May;17(5):694-703. do

Insert

Kir4.1 (Kcnj10 Rat)

Use

AAV; Adeno-associated virus ()

Expression

Mammalian

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

Plasmid

#131003

Purpose

bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

Kv2.1-HA

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)

Plasmid

#197892

Purpose

Can be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

hM4D/2A/GFP

Use

AAV

Mutation

N/A

Promoter

EF1a

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-P(Cry1)-DIO-intron336-dLUC

Plasmid

#110056

Purpose

Cry1 transcription luciferase reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and luciferase (Cry1 Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Cry1

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-SomArchon

Plasmid

#126943

Purpose

Cre-dependent expression of SomArchon under CAG promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_eCBmut

Plasmid

#164605

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCBmut in neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

AAV

Mutation

S383T, F177A

Promoter

hSyn

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 (mIDUA)

Plasmid

#170123

Purpose

AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA (Idua Mouse)

Use

AAV

Expression

Mammalian

Promoter

Human U6 and mouse U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3

Plasmid

#170177

Purpose

Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME2s-ST-P2A-NLS-mRuby3

Use

AAV

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-P(Cry1)-forward-intron336-Venus-NLS-D2

Plasmid

#110054

Purpose

Cry1 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and Venus (Cry1 Mouse)

Use

AAV

Tags

NLS-D2

Expression

Mammalian

Promoter

Cry1

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pHIV-IL13-GAPGlySer-PDGFR

Plasmid

#240247

Purpose

Lentiviral transfer plasmid encoding IL13 with GAP-Gly-Ser linker and PDGFR TM

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_r5-HTmut

Plasmid

#208720

Purpose

Expresses the red 5-HT sensor GRAB_r5-HTmut in neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Red fluorescent 5-HT sensor GRAB_r5-HTmut

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI)-EFS-CasRx-T2A-mCherry-pA

Plasmid

#192481

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

U6/EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Ruby2sm-Flag.WPRE.SV40

Plasmid

#98925

Purpose

AAV expression of Ruby2 spaghetti monster FLAG construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

Ruby2-smFP-Flag

Use

AAV

Expression

Mammalian

Promoter

CBA

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-FRT Kir2.1-2A-GFP

Plasmid

#161577

Purpose

Expression of Kir2.1-2A-GFP in a Flp-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

Kir2.1-2A-GFP (Kcnj2 Mouse)

Use

AAV

Mutation

NA

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ClipF-sesRNA-smV5-tTA2-W3SL

Plasmid

#192067

Purpose

Expression of ClipF, sesRNA in mammalian cells, with smV5 and tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for human FXOP2

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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AAV_NLS-SaCas9-NLS-VPR

Plasmid

#68496

Purpose

AAV vector containing SaCas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

SaCas9-VPR

Use

AAV and CRISPR

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

Plasmid

#82705

Purpose

AAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

Depositor

Jonathan Ploski

Article

de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

Insert

U6/TO gRNA Expression Cassette Containing Tet2 gRNA

Use

AAV and CRISPR

Tags

CMV promoter driving expression of TetR-P2A-GFP-K…

Expression

Mammalian

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa

Plasmid

#233035

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

RfxCas13d-compatible gRNA expression cassette and CasRx

Use

AAV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-oScarlet

Plasmid

#137135

Purpose

Viral expression of oScarlet

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

oScarlet

Use

AAV

Mutation

E95D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Adeno EA

Plasmid

#64071

Purpose

Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Insert

U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9

Use

Adenoviral

Tags

FLAG-Cas9

Promoter

U6 and CBh

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only