We narrowed to 9,400 results for: Coli

1. pET15b-bhaC1

   Plasmid

   #167814

   Purpose

   Expresses BhaC1 in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

   Insert

   BhaC1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSF-1b-S7-S83C/Δ157-178

   Plasmid

   #128592

   Purpose

   Expresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coli

   Depositor

   James Williamson

   Article

   Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

   Insert

   S7 E.coli ribosomal protein (rpsG E.coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S83C, Δ157-178

   Promoter

   T7

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHBT-Mb(GDx-Clo_L10)

   Plasmid

   #183406

   Purpose

   Expresses Monobody GDx-Clo_L10 in E. coli

   Depositor

   Shohei Koide

   Article

   Kermani et al Nat Commun. 2020 Nov 27;11(1):6064. doi:

   Insert

   His tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)
   

   Use

   Tags

   His tag-Avi tag-TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PdnaK-IR2-IR2 GFP pZa

   Plasmid

   #170083

   Purpose

   GFP expression under the control of E. coli dnaK promoter engineered with double IR2 HAIR motif from M. tuberculosis

   Depositor

   Urartu Seker

   Article

   Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

   Insert

   Green fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PdnaK-IR2-IR2

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PdnaK-IR3 GFP pZa

   Plasmid

   #170084

   Purpose

   GFP expression under the control of E. coli dnaK promoter engineered with IR3 HAIR motif from M. tuberculosis

   Depositor

   Urartu Seker

   Article

   Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

   Insert

   Green fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PdnaK-IR3

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PdnaK-IR2 GFP pZa

   Plasmid

   #170081

   Purpose

   GFP expression under the control of E. coli dnaK promoter engineered with IR2 HAIR motif from M. tuberculosis

   Depositor

   Urartu Seker

   Article

   Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

   Insert

   Green fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PdnaK-IR2

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a(+)-HisTim12_CtoA

   Plasmid

   #170281

   Purpose

   Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

   Insert

   yeast Tim12 (TIM12 Budding Yeast)
   

   Use

   Tags

   TEV-cleavable N-terminal His tag (on backbone)

   Expression

   Bacterial

   Mutation

   mutated two non-conserved Cys to Ala

   Promoter

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET19b-rDAD2 cPGFP(N180)

   Plasmid

   #166001

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   rDAD2 cPGFP(N180)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LSSmOrange- V49A (please see depositor comments)

   Promoter

   T7 promoter

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r

   Plasmid

   #162965

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6-Halotag fusion tag with upstream E. coli ribosome binding site
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r

    Plasmid

    #162964

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-tetpA(R1R4)-iRPT

    Plasmid

    #154882

    Purpose

    Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

    Depositor

    Hideyuki Okano

    Article

    Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

    Inserts

    iRFP713
    Puromycin resistance gene
    rtTAM2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-Avi-Coh (E154C)-HIS

    Plasmid

    #153447

    Purpose

    E. coli expression of Rc. CohE construct with E154C mutation.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.CohE
    

    Use

    Tags

    6xHis and Avi-tag

    Expression

    Bacterial

    Mutation

    E154C mutation on CohE

    Promoter

    T7 promoter

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSFDuet1-tglA(C51)HIB

    Plasmid

    #134675

    Purpose

    Expresses His6-TglA(C51), TglH, TglI and TglB in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Inserts

    TglA(C51)
    TglH
    TglI
    TglB
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    insertion of cysteine 51

    Promoter

    T7

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACYCDuet1-ammA(∆50-56)

    Plasmid

    #134684

    Purpose

    Expresses His6-AmmA(∆50-56) in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Insert

    ammA(del50-56)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    deletion of amino acids 50-56

    Promoter

    T7

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHis8-4b-McDAH

    Plasmid

    #140129

    Purpose

    Expresses McDAH with N-terminal 8xHis-tag in BL21 E. coli

    Depositor

    Jing-Ke Weng

    Article

    Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

    Insert

    Dechloroacutumine halogenase from Menispermum canadense
    

    Use

    Tags

    8x-His, TEV protease site (ENLYFQ)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSB4C5_J23101-vdh

    Plasmid

    #128130

    Purpose

    Expressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    vdh
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T1 mouse Tankyrase1 ARC2-3 (308-655)

    Plasmid

    #132637

    Purpose

    expresses mouse Tankyrase1 ARC2-3 (308-655) in E. coli

    Depositor

    Wenqing Xu

    Article

    Morrone et al Proc Natl Acad Sci U S A. 2012 Jan 31;10

    Insert

    Tankyrase1 (308-655)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBEAST_J23101-HipO

    Plasmid

    #128133

    Purpose

    The cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    HipO
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBEAST_J23101-vdh

    Plasmid

    #128134

    Purpose

    The cell-free adapted backbone, pBEAST, expressing vdh (the enzyme converting benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    vdh
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMH-HT_SsCHAD-H29A-R32A-R36A

    Plasmid

    #127786

    Purpose

    Expresses a SsCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    mutations in His29, Arg32 and Arg36 to Ala

    Promoter

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMH-HT_CtCHAD-R438A-K441A

    Plasmid

    #127791

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in Arg4…

    Promoter

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTEI177

    Plasmid

    #126077

    Purpose

    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP1-10 and expression in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    ccdB Cassette (BsaI)
    GFP1-10
    

    Use

    Binary, module 1

    Tags

    linker (GTGGGGS)

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pTEI129

    Plasmid

    #126210

    Purpose

    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    ccdB Cassette (BsaI)
    GFP11x7 (5 aa linker)
    

    Use

    Binary, module 1

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTEI161

    Plasmid

    #126211

    Purpose

    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11 and expression in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    ccdB Cassette (BsaI)
    GFP11
    

    Use

    Binary, module 1

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. BPK2013-BsCas12b

    Plasmid

    #121952

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    BsCas12b
    

    Use

    Tags

    10xHis tag and 3xHA tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET15b+R2(L171C)

    Plasmid

    #108239

    Purpose

    Expresses PdR-PCNA2(L171C) fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA2(L171C) fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    PCNA2,L171C

    Promoter

    T7 promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. CTL2 (M2225R)

    Plasmid

    #96952

    Purpose

    Expresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli

    Depositor

    Douglas Rees

    Article

    Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

    Insert

    CTL2 (M222R) of PIEZO1
    

    Use

    Tags

    6x Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pRSM13

    Plasmid

    #101644

    Purpose

    Encodes the fluorescent protein meleCFP for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    meleCFP
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET21a - TryX

    Plasmid

    #89623

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi tryparedoxin protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Tryparedoxin
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPD3cC

    Plasmid

    #86972

    Purpose

    Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …

    Promoter

    T7 promoter

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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