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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)

Plasmid

#162822

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF3b(CoV1h)

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF3b(CoV1h)(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

Plasmid

#162805

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA_IRES_Blasticidin

Use

Piggybac

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pUC_h7SK M15 (CUA)

Plasmid

#154784

Purpose

plasmid with single copy of Mma PylT M15 mutant (CUA anticodon)

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylT

Expression

Mammalian

Mutation

G10A, G14U, G16U, C20U, C52A

Promoter

h7SK

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pWaldo-GFPe_PepTSo

Plasmid

#58334

Purpose

Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

Depositor

So Iwata, Simon Newstead

Article

Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

Insert

PepTso

Tags

GFP-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
[#GS5-RV] MitoTRACER-Donor

Plasmid

#233505

Purpose

Retroviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Use

Retroviral and Synthetic Biology

Tags

GFP11 and HA Tag

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Kir2.1-T2A-tdTomato

Plasmid

#60598

Purpose

Expresses Kir2.1-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Kir2.1-T2A-tdTomato (Kcnj2 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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UAS:mCherry

Plasmid

#170994

Purpose

UAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

UAS:mCherry

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

Synthetic 14x UAS:Gal4

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Kir2.1Mut-T2A-tdTomato

Plasmid

#60644

Purpose

Expresses Kir2.1Mut-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Kcnj2 (Kcnj2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

GYG to AAA (aa144-146)

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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mDlx-SynTagMA_post-2A-mCerulean

Plasmid

#130899

Purpose

Synaptic Tag for Mapping Activity in GABAergic neurons

Depositor

Simon Wiegert

Article

Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

Insert

SynTagMA

Use

AAV

Tags

mCerulean

Expression

Mammalian

Promoter

mDlx

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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VEE-mScarlet3-IRES-EGFP-IRES-moxBFP

Plasmid

#242407

Purpose

Native (conventional) self-amplifying RNA (saRNA) control construct expresses moxBFP as visual marker of IRES functionality

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

mScarlet3
IRES-EGFP
IRES-moxBFP

Use

Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTriEx4-H6-FGAm (FlincG3)

Plasmid

#49202

Purpose

Fluorescent reporter for cGMP

Depositor

John Garthwaite

Article

Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

Insert

FlincG3

Tags

His tag + other tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Met to Lys in cpEGFP region similar to CGaMP3

Promoter

CMV

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pLexM-SLC3A2

Plasmid

#197994

Purpose

Expresses SLC3A2 as an N-terminal His tagged construct in mammalian cells

Depositor

Simon Newstead

Article

Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

Insert

SLC3A2 (SLC3A2 Human)

Tags

His tag

Expression

Mammalian

Mutation

Please see depositor comments below

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGG-GFP-TurboID-3XNLS

Plasmid

#209400

Purpose

Transiently expressing and visualizating the subcellular localization of TurboID fused with 3X nuclear localization signal (NLS) in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

3XHA-TurboID-3XNLS

Tags

GFP

Expression

Plant

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLexM-SLC7A11_GFP

Plasmid

#197995

Purpose

Expresses SLC7A11 with an N-terminal FLAG tag and C-terminal TEV-GFP-His8 tag in mammalian cells

Depositor

Simon Newstead

Article

Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

Insert

SLC7A11 (SLC7A11 Human)

Tags

FLAG tag and TEV-GFP-His8

Expression

Mammalian

Promoter

CMV

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalA (short) wt

Plasmid

#19723

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

NOTE: short splice variant Long Sp. Variant: MV…

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

Plasmid

#194925

Purpose

mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho

Depositor

Hao Xu

Article

Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1

Type

Empty backbone

Use

CRISPR

Promoter

hPGK

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_sfGPF 150TAG

Plasmid

#154765

Purpose

plasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA

Plasmid

#235678

Purpose

Plasmid control for pcDNA3.1-HA-RAD52-WT and pcDNA3.1-HA-RAD52-∆C

Depositor

Simon Powell

Article

Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAS

Plasmid

#140008

Purpose

Plasmid with 4xMmaPylT cassette to receive genes of interest for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

4xPylT (Mma) cassette

Expression

Mammalian

Mutation

U25C

Promoter

h7SK

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB501

Plasmid

#74843

Purpose

Gateway cloning compatible binary vector for simple cloning of gene.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-SaCas9

Plasmid

#178901

Purpose

SaCas9 (Cas9 from Staphylococcus aureus)

Depositor

Hendrik Milting

Article

Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

Insert

SaCas9

Tags

His6-Tag, bipartite nuclear localization signal f…

Expression

Bacterial

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB401

Plasmid

#74795

Purpose

Gateway cloning compatible binary vector for simple cloning of gene.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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PRS_8-GCaMP8m

Plasmid

#210400

Purpose

AAV-vector for fluorescent calcium measurements in the green spectrum from noradrenergic neurons

Depositor

Simon Wiegert

Article

Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

Has Service

AAV2

Insert

GCaMP8m

Use

AAV

Expression

Mammalian

Promoter

PRSx8

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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mcherry Beta 1

Plasmid

#36111

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein beta subunit B1 (GNB1 Bovine)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pAS_FLAG-Mma PylRS

Plasmid

#154762

Purpose

plasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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ArcLight-Q239

Plasmid

#36856

Depositor

Vincent Pieribone

Article

Jin et al Neuron. 2012 Sep 6;75(5):779-85.

Insert

fluorescent protein voltage sensor

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

simian CMV

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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PRS_8-eGFP

Plasmid

#192589

Purpose

AAV-vector for fluorescent labeling of noradrenergic neurons

Depositor

Simon Wiegert

Article

Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

PRSX8

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pHRSin_hTCRαγεζ

Plasmid

#187351

Purpose

Lentiviral expression of GPa3b17 hTCR alpha, and truncated hCD3 (gamma, epsilon, and zeta)

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR alpha, and truncated hCD3 (gamma, epsilon, and zeta)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMD19T-psba1-TetR-PL22-dCas9-SpR

Plasmid

#73223

Purpose

Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

Depositor

Paul Hudson

Article

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

dCas9 from S. pyogenes

Tags

c-myc

Expression

Bacterial

Mutation

Silent mutation at bp 1341 A->C to remove an E…

Promoter

PL22

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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YFP B1 in pcDNA3.1

Plasmid

#36397

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein B1 (GNB1 Bovine)

Tags

YFP

Expression

Mammalian

Promoter

cmv

Available Since

April 4, 2013

Availability

Academic Institutions and Nonprofits only