We narrowed to 21,628 results for: ESC

1. pMSCV-miR30-shJAG1 #4

   Plasmid

   #171197

   Purpose

   Retroviral vector for U6 promoter driven expression empty miR30 based shJAG1 #4 (to be used in conjunction with Phoenix packaging cells).

   Depositor

   Masashi Narita

   Article

   Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

   Insert

   shJAG1 #4
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-mGFAP(ABC1D)-ECS-SpyCatcher003

   Plasmid

   #234545

   Purpose

   to express SpyCatcher on the extracellular side of astrocytic plasmamembrane

   Depositor

   Hajime Hirase

   Article

   Vittani et al bioRxiv 2025.03.18.643859

   Insert

   ECS-SC
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   mGFAP(ABC1D)

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-mGFAP(ABC1D)-ECS-dSpyCatcher003

   Plasmid

   #234546

   Purpose

   to express dSpyCatcher on the extracellular side of astrocytic plasmamembrane

   Depositor

   Hajime Hirase

   Article

   Vittani et al bioRxiv 2025.03.18.643859

   Insert

   ECS-dSC
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   mGFAP(ABC1D)

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLUEF1 Luc2-IRES-GFP

   Plasmid

   #224467

   Purpose

   Ef1a downstream of a ubiquitous chromatin opening element drives expression of Luciferase2 and IRES GFP

   Depositor

   Robert Kerbel

   Article

   Khan et al Cancer Res. 2025 Apr 14. doi: 10.1158/00

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAX1_HS_gpN:SnoopTag

   Plasmid

   #225195

   Purpose

   Used for adding C-terminal SnoopTag to the E. coli HS P2 phage major capsid protein

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   gpN homology arms and SnoopTag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAX1_HS∆tum

   Plasmid

   #225198

   Purpose

   Used to create a markerless deletion of the E. coli HS tum gene

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   tum homology arms
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAX1_HS_lexA(Ind-)

   Plasmid

   #225199

   Purpose

   Used to create a non-inducible lexA by introducing a G85D mutation in E. coli HS

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   lexA homology arms
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   lexA G85D

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAX1_HS_gpN:SpyTag

   Plasmid

   #225193

   Purpose

   Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   gpN homology arms and SpyTag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAX1_HS_gpN:SpyTag003

   Plasmid

   #225194

   Purpose

   Used for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid protein

   Depositor

   Travis Wiles

   Article

   Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

   Insert

   gpN homology arms and SpyTag003
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EGFP

    Plasmid

    #232754

    Purpose

    Expresses EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GB4635

    Plasmid

    #215263

    Purpose

    Module for the expression of HISPS under the copper inducible CBS:mDFR promoter and H3H under the red light regulated Etr8:CMVmin promoter and the constitutive expression of LUZ, CPH,EGFP and p19

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    CBS4x:mDFR:HISPS-Etr4:mDFR:H3HSF-LUZ-CPH-EGFP-p19
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p25.pA.NGFR.mCMV.hPGK.Rab35(WT).wpre[sequenced]

    Plasmid

    #226764

    Purpose

    rab35 (WT)

    Depositor

    Ferdinando Pucci

    Article

    Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

    Insert

    rab35 (RAB35 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-GPHN-FingR-mGold-CCR5TC

    Plasmid

    #231784

    Purpose

    Visualization of inhibitory post-synapse using GPHN-FingR-mGold

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    GPHN-FingR-mGold-CCR5TC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Actin promoter with CMV enhancer

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-GPHN-FingR-mVenus-CCR5TC

    Plasmid

    #231783

    Purpose

    Visualization of inhibitory post-synapse using GPHN-FingR-mVenus

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    GPHN-FingR-mVenus-CCR5TC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Actin promoter with CMV enhancer

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-GPHN-FingR-mGold2t-CCR5TC

    Plasmid

    #231786

    Purpose

    Visualization of inhibitory post-synapse using GPHN-FingR-mGold2t

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    GPHN-FingR-mGold2t-CCR5TC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Actin promoter with CMV enhancer

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-mGold2t-Rab4a-C-7

    Plasmid

    #231781

    Purpose

    Visualization of endosome in mammalian cells using mGold2t

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2t-Rab4a-C-7 (RAB4A Human)
    

    Use

    Tags

    mGold2s

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-mGold2s-Rab4a-C-7

    Plasmid

    #231773

    Purpose

    Visualization of endosome in mammalian cells using mGold2s

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2s-Rab4a-C-7 (RAB4A Human)
    

    Use

    Tags

    mGold2s

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSF-G1(7CNW)RS

    Plasmid

    #222874

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-HA-GTSE1-S724A

    Plasmid

    #224735

    Purpose

    For expression of HA-tagged GTSE1 protein with S724A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 724 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-HA-GTSE1-S734A

    Plasmid

    #224736

    Purpose

    For expression of HA-tagged GTSE1 protein with S734A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 734 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1-HA-GTSE1-S171A

    Plasmid

    #224723

    Purpose

    For expression of HA-tagged GTSE1 protein with S171A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 171 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-HA-GTSE1-S187A

    Plasmid

    #224724

    Purpose

    For expression of HA-tagged GTSE1 protein with S187A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 187 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-HA-GTSE1-S262A

    Plasmid

    #224725

    Purpose

    For expression of HA-tagged GTSE1 protein with S262A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 262 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-HA-GTSE1-S345A

    Plasmid

    #224726

    Purpose

    For expression of HA-tagged GTSE1 protein with S345A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 345 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-HA-GTSE1-S417A

    Plasmid

    #224727

    Purpose

    For expression of HA-tagged GTSE1 protein with S417A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 417 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-HA-GTSE1-S480A

    Plasmid

    #224728

    Purpose

    For expression of HA-tagged GTSE1 protein with S480A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 480 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-HA-GTSE1-S555A

    Plasmid

    #224729

    Purpose

    For expression of HA-tagged GTSE1 protein with S555A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 555 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-HA-GTSE1-S594A

    Plasmid

    #224730

    Purpose

    For expression of HA-tagged GTSE1 protein with S594A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 594 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-HA-GTSE1-S599A

    Plasmid

    #224731

    Purpose

    For expression of HA-tagged GTSE1 protein with S599A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 599 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-HA-GTSE1-S611A

    Plasmid

    #224732

    Purpose

    For expression of HA-tagged GTSE1 protein with S611A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 611 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1-HA-GTSE1-S707A

    Plasmid

    #224733

    Purpose

    For expression of HA-tagged GTSE1 protein with S707A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 707 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1-HA-GTSE1-S718A

    Plasmid

    #224734

    Purpose

    For expression of HA-tagged GTSE1 protein with S718A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 718 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1-HA-GTSE1-S84A

    Plasmid

    #224720

    Purpose

    For expression of HA-tagged GTSE1 protein with S84A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 84 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-HA-GTSE1-S91A

    Plasmid

    #224721

    Purpose

    For expression of HA-tagged GTSE1 protein with S91A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 91 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1-HA-GTSE1-S157A

    Plasmid

    #224722

    Purpose

    For expression of HA-tagged GTSE1 protein with S157A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 157 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRSF-G1(5CNW)RS

    Plasmid

    #222872

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV hGRN2

    Plasmid

    #213683

    Purpose

    Expresses human granulin-2 with an N-terminal twin-Strep-FLAG tag

    Depositor

    Thomas Kukar

    Article

    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

    Insert

    human Granulin-2
    

    Use

    AAV

    Tags

    twin-Strep-FLAG

    Expression

    Mutation

    Promoter

    cytomegalovirus enhancer/chicken β-actin promoter

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pIN1 (gpdA-cTerm-mGL-ptrA)

    Plasmid

    #188735

    Purpose

    Expresses mGreenlantern for C-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-mGreenlantern PtrA
    

    Use

    Filamentous fungal expression

    Tags

    mGreenlantern

    Expression

    Mutation

    Promoter

    gpdA

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pIN2 (gpdA-nTerm-mGL-ptrA)

    Plasmid

    #188736

    Purpose

    Expresses mGreenlantern for N-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-mGreenlantern PtrA
    

    Use

    Filamentous fungal expression

    Tags

    mGreenlantern

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pIN3 (gpdA-cTerm-Citrine2-ptrA)

    Plasmid

    #188737

    Purpose

    Expresses Citrine2 for C-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-Citrine2 PtrA
    

    Use

    Filamentous fungal expression

    Tags

    Citrine2

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pIN4 (gpdA-nTerm-Citrine2-ptrA)

    Plasmid

    #188738

    Purpose

    Expresses Citrine2 for N-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-Citrine2 PtrA
    

    Use

    Filamentous fungal expression

    Tags

    Citrine2

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pIN5 (gpdA-cTerm-mTurqoise2-ptrA)

    Plasmid

    #188739

    Purpose

    Expresses mTurqoise2 for C-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-mTurquoise PtrA
    

    Use

    Filamentous fungal expression

    Tags

    mTurquoise

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pIN6 (gpdA-nTerm-mTurqoise2-ptrA)

    Plasmid

    #188740

    Purpose

    Expresses mTurqoise2 for N-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-mTurqoise2 PtrA
    

    Use

    Filamentous fungal expression

    Tags

    mGreenlantern

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAAV-CAG-UnaG-P2A-FusionRed

    Plasmid

    #191109

    Purpose

    Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    UnaG
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    chicken β-actin promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pQE30-DARPin-E11

    Plasmid

    #226784

    Purpose

    Expression of His-tagged DARPin E11 in E. coli

    Depositor

    Sebastian Mathea

    Article

    Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

    Insert

    DARPin E11
    

    Use

    Tags

    FLAG and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pcDNA3-FLAG3-drAgo2D-A

    Plasmid

    #221734

    Purpose

    Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2D-A (ago2 Zebrafish)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D683A in active site

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA3-FLAG3-drAgo2Y-F

    Plasmid

    #221732

    Purpose

    Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2Y-F (ago2 Zebrafish)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Y680F in active site

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA3-FLAG3-drAgo2D-E

    Plasmid

    #221731

    Purpose

    Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2D-E (ago2 Zebrafish)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D651E in active site

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA3-FLAG3-drAgo2

    Plasmid

    #221730

    Purpose

    Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2 (ago2 Zebrafish)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCS2+-hsAgo2

    Plasmid

    #221718

    Purpose

    Expression of human Ago2 in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsAgo2 (AGO2 Human)
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge