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pHH0103_DNMBP-6/6

Plasmid

#91489

Purpose

Protein expression and purification of human SH3 domain construct DNMBP-6/6

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DNMBP-6/6 (DNMBP Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Opto-GPR119

Plasmid

#106040

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR119; Opto-GPR119; D6)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR119 (GPR119 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RL3m6L2

Plasmid

#109366

Purpose

Human rod opsin chimera with intracellular loop 3 replaced by intracellular loop 2 of mGluR6 with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rhodopsin chimera with mGluR6 intracellular loop 2 at intracellular loop 3 position (GRM6, RHO Human)

Tags

1D4

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-actin-G168D,Y169D

Plasmid

#118384

Purpose

Gateway compatible donor vector with actin-G168D,Y169D.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

G168D,Y169D

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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GST-ENLYFQC-PNT(46-138)-His6

Plasmid

#42919

Depositor

Barbara Imperiali

Article

Lukovic et al Angew Chem Int Ed Engl. 2009;48(37):6828

Insert

Ets-1 PNT domain (46-138) (Ets1 Mouse)

Tags

6xHis, GST, and TEV protease cleavage site

Expression

Bacterial

Mutation

Contains the PNT domain (Serine 46 through Lysine…

Promoter

Ptac

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-2

Plasmid

#112842

Purpose

transient/stable overexpression of the central domain of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

C1 domain of Catulin

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-NoStart

Plasmid

#49853

Purpose

Encodes human connexin 43 with the start methionine codon mutated to isoleucine and with wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Start codon mutated to from methionine to isoleuc…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
pCR2.1-INS-904

Plasmid

#53970

Purpose

904 bp insert from human INS gene used as a control for methylation-specific PCR assays

Depositor

Kevin Ferreri

Article

Husseiny et al PLoS One. 2012;7(10):e47942. doi: 10.137

Insert

human INS gene partial (INS Human)

Expression

Bacterial

Promoter

lac

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pHH0103_LASP1-1/1-SV2/2

Plasmid

#91392

Purpose

Protein expression and purification of human SH3 domain construct LASP1-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LASP1-1/1-SV2/2 (LASP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-T830E

Plasmid

#91996

Purpose

Expresses FLAG-HA-AGO2 (T830E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

T830E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S834E

Plasmid

#91998

Purpose

Expresses FLAG-HA-AGO2 (S834E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S834E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824A

Plasmid

#91987

Purpose

Expresses FLAG-HA-AGO2 (S824A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-T830A

Plasmid

#91988

Purpose

Expresses FLAG-HA-AGO2 (T830A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

T830A

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S831A

Plasmid

#91989

Purpose

Expresses FLAG-HA-AGO2 (S831A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S831A

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S834A

Plasmid

#91990

Purpose

Expresses FLAG-HA-AGO2 (S834A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S834A

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S828A

Plasmid

#91991

Purpose

Expresses FLAG-HA-AGO2 (S828A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828A

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824E

Plasmid

#91994

Purpose

Expresses FLAG-HA-AGO2 (S824E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S828E

Plasmid

#91995

Purpose

Expresses FLAG-HA-AGO2 (S828E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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LXSN-Cx43-Myc

Plasmid

#60694

Purpose

retroviral expression of Myc-tagged rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43-YA/VD-Myc

Plasmid

#60702

Purpose

retroviral expression of Myc tagged rat Cx43 YA/VD mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

YA/VD (Y286A/V289D)

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only