We narrowed to 24,612 results for: Nes

1. shMEK1/2 v2 neo

   Plasmid

   #72570

   Purpose

   Hairpin targeting MEK1 and MEK2 (murine).

   Depositor

   Kevin Janes

   Article

   Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

   Insert

   MEK1/2 (Map2k1, Map2k2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpCas9-Puro HLAG-2A-sgRNA

   Plasmid

   #182551

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2A-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pENTR221-GLI1

   Plasmid

   #79497

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human GLI1 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   GLI1 (GLI1 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SLK_HUMAN_D0

   Plasmid

   #79733

   Purpose

   This plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, John Chodera, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   SLK (SLK Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pET28a-FAM86A

   Plasmid

   #85117

   Purpose

   bacterial expression of FAM86A

   Depositor

   Pal Falnes

   Article

   Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

   Insert

   EEF2KMT (FAM86A Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRD395

   Plasmid

   #163102

   Purpose

   Expression of mCherry_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   mCherry_H-NSdbd
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. tet-pLKO shMKP5.v2 puro

   Plasmid

   #87793

   Purpose

   Expresses an inducible short hairpin targeting human MKP5 sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   shMKP5 (DUSP10 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE tight

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. tet-pLKO shMKP5.v1 puro

   Plasmid

   #87792

   Purpose

   Expresses an inducible short hairpin targeting human MKP5 sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   shMKP5 (DUSP10 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE tight

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pB-RTP1S-Integration

   Plasmid

   #129458

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses RTP1S

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   RTP1 (Rtp1 Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Removal of the first 36 amino acids of the protein

   Promoter

   TRE (Tet-On)

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLX302 PCSK5-V5 puro

    Plasmid

    #98389

    Purpose

    Lentiviral vector for constitutive expression of human PCSK5 with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    PCSK5 (PCSK5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-CAG-DIO-erHRP(N175S mutant)

    Plasmid

    #79906

    Purpose

    Adeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)

    Depositor

    Joshua Sanes

    Article

    Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

    Insert

    erHRP(N175S mutant)
    

    Use

    AAV and Cre/Lox

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    N175S mutation

    Promoter

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HeFSpCas9

    Plasmid

    #92355

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR221-CCL5.Pro

    Plasmid

    #79492

    Purpose

    MultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoter

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    CCL5 Promoter (CCL5 Synthetic, Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    CCL5 Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. tet-pLKO shMKP1.v2 puro

    Plasmid

    #87791

    Purpose

    Expresses an inducible short hairpin targeting human MKP1 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP1 (DUSP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEasyG3_hph

    Plasmid

    #184919

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEN_TT JunD-HA

    Plasmid

    #58498

    Purpose

    Gateway entry vector for an inducible HA-tagged mouse JunD

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    JunD (Jund Mouse)
    

    Use

    Entry vector

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pVenus-CyclinB1(R42A) (1317)

    Plasmid

    #39873

    Depositor

    Jonathon Pines

    Article

    Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

    Insert

    CyclinB1(R42A) (CCNB1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    R42A = destruction box mutations = non-destructib…

    Promoter

    Available Since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA5-FRT/TO-neo-C11orf51-IRES2-mRuby (2763)

    Plasmid

    #39858

    Depositor

    Jonathon Pines

    Article

    Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

    Insert

    C11orf51 (ANAPC15 Human)
    

    Use

    Tags

    IRES2-mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pRSET a sfMatryoshCaMP6s-T78H

    Plasmid

    #100021

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfMatryoshCaMP6s
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP and T78H m…

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330-Flag-eSpCas9 (without sgRNA)

    Plasmid

    #92354

    Purpose

    Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge