161,570 results

1. HRE-doUnaG

   Plasmid

   #201713

   Purpose

   Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequence

   Depositor

   Friedemann Kiefer

   Article

   Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

   Insert

   UnaG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Cerulean-2xFv-hRIPK3

   Plasmid

   #106079

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   Cerulean-2xFv-hRIPK3 (RIPK3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pCS2+mNeonGreen-C Cloning Vector

   Plasmid

   #128144

   Purpose

   To express a protein of interest fused to the C-terminus of mNeonGreen

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   mNeonGreen fusion

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAGGS puro-GFP-BamHI-IRES-mCherry

   Plasmid

   #232247

   Purpose

   AcGFP1-BamHI-IRES-mCherry empty backbone

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

   Plasmid

   #85452

   Purpose

   Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

   Depositor

   Aviv Regev

   Article

   Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

   Insert

   U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-GFP11(x7)-Actin

   Plasmid

   #181967

   Purpose

   Human beta actin tagged with 7 copies of GFP11.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   GFP11(x7)-Actin (ACTB )
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

   Plasmid

   #138152

   Purpose

   Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   eGFP-MCS
   

   Use

   Lentiviral

   Tags

   2PEST AND NLS

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMKV057

   Plasmid

   #133312

   Purpose

   BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, WUS2 and IPT

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc+. WUS2, IPT
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUbi10, Nos, 35S

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV_ABEmax

   Plasmid

   #112095

   Purpose

   A:T-to-G:C base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   ABEmax
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLD-puro-Cc-PRDX5WT-VA

    Plasmid

    #98688

    Purpose

    Lentiviral expression of human PRDX5-WT in mammalian cells

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    PRDX5 (PRDX5 Human)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro

    Plasmid

    #211468

    Purpose

    Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    4xGFP11-SEC61B
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. v3em-Nterm-PE2max

    Plasmid

    #198734

    Purpose

    AAV genome encoding N-terminal PE2max

    Depositor

    David Liu

    Article

    Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

    Insert

    Nterm PE2max-NpuN
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Cbh

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOTTC840 - pAAV 5xATF6 secNLuc

    Plasmid

    #82497

    Purpose

    Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

    Depositor

    Brandon Harvey

    Article

    Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

    Insert

    secNLuc
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5x(ATF6 binding site) c-Fos minimal promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC57-TPNOX

    Plasmid

    #87853

    Purpose

    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

    Insert

    TPNOX
    

    Use

    Tags

    C-terminal FLAG tag with a linker

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAcBac1.tR4-AcdRS82

    Plasmid

    #174101

    Purpose

    Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.

    Depositor

    Ryan Mehl

    Article

    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

    Insert

    M. barkeri tRNA synthetase for acridone82
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N311S, C313G, V366A, W382T

    Promoter

    CMV

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p23-puro-EGFP-RPA194

    Plasmid

    #179237

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    RPA194 (POLR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRR.1

    Plasmid

    #69995

    Purpose

    Mammalian expression of cytochrome p450 CYP2B6

    Depositor

    Kathrin Klein

    Article

    Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

    Insert

    CYP2B6 (CYP2B6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GPI-mCherry2

    Plasmid

    #182869

    Purpose

    Monomer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    GPI-mCherry2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXG237

    Plasmid

    #141281

    Purpose

    ATF4 translational reporter

    Depositor

    Martin Kampmann

    Article

    Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

    Insert

    uORF1/2 of ATF4 followed by mApple
    

    Use

    Lentiviral

    Tags

    mApple

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SIV_Gag_Pol

    Plasmid

    #236243

    Purpose

    3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Gag and Pol

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    SIV Gag and Pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge