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We narrowed to 12,214 results for: LEA

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Showing: 4461 - 4480 of 12214 results

  3. Polymerase expression - pCMV-Phi29 (LM2708)

    Plasmid
    #208965
    Purpose
    Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Phi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208965-plasmid-map-sequence-id-415813

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  5. PAGFP_NS2B3

    Plasmid
    #195755
    Purpose
    Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
    Depositor
    Partha Mondal
    Publication
    Varghese et al bioRxiv 2022.12.18.520514
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dengue (Type-2) NS2B3
    Tags
    PA-GFP
    Expression
    Mammalian
    Mutation
    No mutations
    Promoter
    CMV
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195755-plasmid-map-sequence-id-384111

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  7. HA-GFP1-10-actin-pcDNA4-TO

    Plasmid
    #118370
    Purpose
    Bimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.
    Depositor
    Maria Vartiainen
    Publication
    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    actin beta (ACTB Human)
    Tags
    HA-GFP1-10
    Expression
    Mammalian
    Promoter
    CMV/TO
    Available since
    May 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    118370-plasmid-map-sequence-id-229456

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  8. pCEP4-Q842gp160

    Plasmid
    #100931
    Purpose
    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 strain
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (Q842.d12) Env
    Tags
    CD5 leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    100931-plasmid-map-sequence-id-202601

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  10. pcDNA3-RAB-AKARev(T/A)

    Plasmid
    #118479
    Purpose
    Negative-control mutant for RAB-AKARev biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB-AKARev(T/A)
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    Dec. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118479-plasmid-map-sequence-id-230370

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  11. BC-Sig-GPRC

    Plasmid
    #44201
    Depositor
    Lei Xu
    Insert
    human GPR56 C-terminal fragment with signal peptide (ADGRG1 Human)
    Use
    Retroviral
    Tags
    none
    Expression
    Mammalian
    Mutation
    The C-terminal fragment of GPR56, starting at am…
    Promoter
    LTR
    Available since
    July 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    44201-plasmid-map-sequence-id-60060

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  16. pBJG1 T7-8xHis-OGT 5N5A

    Plasmid
    #154280
    Purpose
    Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Publication
    Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; bact…
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154280-plasmid-map-sequence-id-304840

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  17. pGBW-m4258306

    Plasmid
    #154795
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;V5
    Expression
    Mammalian
    Mutation
    H. sapiens recode 1
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154795-plasmid-map-sequence-id-311546

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  18. pcDNA3-Venus-cpVenus-FLARE-Cameleon

    Plasmid
    #123350
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus-cpVenus-FLARE-Cameleon
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123350-plasmid-map-sequence-id-307103

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  19. pHtrA-TEV-His12

    Plasmid
    #137036
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137036-plasmid-map-sequence-id-275699

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Showing: 4461 - 4480 of 12214 results