We narrowed to 34,855 results for: CaS;

1. RCAS(A) mWnt1 (CT#165)

   Plasmid

   #14017

   Depositor

   Cliff Tabin

   Insert

   Wnt1 (Wnt1 Mouse)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA-Flag-EcoCas6

   Plasmid

   #106274

   Purpose

   Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   EcoCasE
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_rSyn1 sgRNA / hSpCas9

   Plasmid

   #172840

   Purpose

   Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. D. vulgaris Cas5c/pHMGWA

   Plasmid

   #81182

   Purpose

   Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

   Insert

   Cas5c
   

   Use

   CRISPR

   Tags

   6xHis - MBP - TEV protease site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. D. vulgaris Cas8c/pHMGWA

   Plasmid

   #81183

   Purpose

   Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

   Insert

   Cas8c
   

   Use

   CRISPR

   Tags

   6xHis - MBP - TEV protease site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. RCAS(A) cWnt5b (CT#851)

   Plasmid

   #13975

   Depositor

   Cliff Tabin

   Article

   Theodosiou et al Dev Biol. 2003 Jul 15. 259(2):258-71.

   Insert

   Wnt5b (WNT5B Chicken)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pCJH031_proD_LbCas12a-gRNA-GFP_CAM

   Plasmid

   #183075

   Purpose

   Plasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   LbCas12a guide RNA targeting GFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

   Plasmid

   #107731

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

   Plasmid

   #107728

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMS25: pTns(PmcCAST)

    Plasmid

    #168158

    Purpose

    Inducible expression of PmcCAST TnsAB, TnsC, TniQ and TnsD proteins.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
    PmcTniQ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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