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pEGFP-hGABARAP

Plasmid

#87871

Purpose

Express a EGFP-human GABARAP in mammalian cells

Depositor

Eiki Kominami, Isei Tanida

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

GABARAP (GABARAP Human)

Expression

Mammalian

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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HA-MDFI

Plasmid

#207836

Purpose

Tagged MDFI for western blotting and imaging

Depositor

Charles Cox

Article

Zhou et al Science. 2023 Aug 18;381(6659):799-804.

Insert

MDFI (MDFI Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NPY pHCitron

Plasmid

#235175

Purpose

Plasmid expressing NPY tag with pH sensor, pHCitron (pH-Lemon with mCitrine), in mammalian cells

Depositor

Kate White

Article

Lin et al ACS Sens. 2024 Nov 22;9(11):6032-6039. d

Insert

NPY-pHCitron (NPY Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pRhobin2-SEC61B-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239024

Purpose

Co-overexpression of Rhobin2-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

Rhobin2-SEC61B (SEC61B Synthetic, Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.23 WT 2014 EBOV + Mucin-Like Domain

Plasmid

#85794

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEvol-pAcFRS.1.t1

Plasmid

#73545

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAcFRS.1.t1
pAcFRS.1.t1

Expression

Bacterial

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMJA285= pSin-TCRab-CMVp-Puro

Plasmid

#116875

Purpose

stable TCR expression in human T cells

Depositor

Marcos Alcocer

Article

Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

Insert

TCR (TRAV10, TRBV25-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

deletion of EF1-a promotor

Promoter

CMV Promotor

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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HT103_FCMV_QuasAr6a_Citrine

Plasmid

#178822

Purpose

Expresses an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6a carrying a Citrine expression tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6a_Citrine

Use

Lentiviral

Tags

citrine

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAGM22082_cRed

Plasmid

#117225

Purpose

pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

Insert

Canthaxanthin Operon

Tags

34 amino acid N-terminal linker, Hexahistidine Ta…

Expression

Bacterial

Mutation

removed Type IIS restriction sites within the bac…

Promoter

T7 RNA Polymerase

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3−3xFLAG−AGO2(D669A)

Plasmid

#220461

Purpose

Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Aspartate 669 to alanine

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pFRT-DestFLAGHA_RBPMS

Plasmid

#65743

Purpose

constitutively expresses RBPMS in mammalian cells

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
TFORF1788

Plasmid

#143847

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TCERG1 (TCERG1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)

Plasmid

#216531

Purpose

Targeting vector for CRISPRd generation

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

ABI-dCas9-mCherry

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pQTEV-LOC57019

Plasmid

#34811

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

cytokine induced apoptosis inhibitor 1 (CIAPIN1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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MSCV PIG Erg

Plasmid

#66984

Purpose

Retroviral expression plasmid for mouse Erg expression; containing bicistronic IRES-GFP

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Erg (Erg Mouse)

Use

Retroviral

Promoter

LTR

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV mSin1.1 HA

Plasmid

#12582

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.1 (MAPKAP1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Nov. 22, 2006

Availability

Academic Institutions and Nonprofits only
POLR2A-N CRISPR pX330

Plasmid

#124495

Purpose

A CRISPR plasmid for targeting the N-terminus coding region of human POLR2A

Depositor

Masato Kanemaki

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

POLR2A targeting CRISPR

Use

CRISPR

Promoter

U6 promoter

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV mSin1.2 myc

Plasmid

#12577

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.2 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV mSin1.5 myc

Plasmid

#12579

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.5 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pCMV4Neo-murine IL-17RC(FL)

Plasmid

#46864

Purpose

expresses mouse IL-17RC (all exons)

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RC (Il17rc Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only

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