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Showing: 4481 - 4500 of 5817 results

  5. GCN5 core-dCas9-p300 core

    Plasmid
    #179553
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179553-plasmid-map-sequence-id-354783

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  7. pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)

    Plasmid
    #217014
    Purpose
    Expression of CDK2-7 chimera in E.coli
    Depositor
    Manjeet Mukherjee
    Article
    Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018
    Insert
    CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N) (CDK7, CDK2 Human)
    Tags
    C3 protease cleavable GST tag
    Expression
    Bacterial
    Mutation
    CDK2 (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)
    Available Since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217014-plasmid-map-sequence-id-431050

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  8. GCN5 FL-dCas9-p300 core

    Plasmid
    #179554
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179554-plasmid-map-sequence-id-354754

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  9. HisGB1-Fusion + CBP TAZ1(340-439)

    Plasmid
    #173762
    Purpose
    Bacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructs
    Depositor
    Peter Wright
    Article
    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(
    Inserts
    CITED2(216-246):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    Tags
    His6GB1 and thrombin
    Expression
    Bacterial
    Mutation
    fusion of CITED2 and Hif1a
    Promoter
    T7
    Available Since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    173762-plasmid-map-sequence-id-353460

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  10. HisGB1-FusionL21A + CBP TAZ1(340-439)

    Plasmid
    #173763
    Purpose
    Bacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs
    Depositor
    Peter Wright
    Article
    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(
    Inserts
    CITED2(216-246L231A):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    Tags
    His6GB1 and thrombin
    Expression
    Bacterial
    Mutation
    fusion of CITED2 and Hif1a
    Promoter
    T7
    Available Since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    173763-plasmid-map-sequence-id-353459

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  11. HIsGB1-FusionL63A + CBP TAZ1(340-439)

    Plasmid
    #173764
    Purpose
    Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs
    Depositor
    Peter Wright
    Article
    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(
    Inserts
    CITED2(216-246):Hif1a(796-826L822A) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    Tags
    His6GB1 and thrombin
    Expression
    Bacterial
    Mutation
    fusion of CITED2 and Hif1a
    Promoter
    T7
    Available Since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    173764-plasmid-map-sequence-id-353458

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  15. pLenti-EML4-ALK variant 3a I1171N

    Plasmid
    #183832
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    I1171N
    Promoter
    CMV
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    183832-plasmid-map-sequence-id-360487

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  16. pLenti-EML4-ALK variant 3a G1202R

    Plasmid
    #183830
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    G1202R
    Promoter
    CMV
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    183830-plasmid-map-sequence-id-360489

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  17. pLenti-EML4-ALK variant 3a L1196M/G1202R

    Plasmid
    #183831
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    L1196M/G1202R
    Promoter
    CMV
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183831-plasmid-map-sequence-id-360488

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  18. pLenti-EML4-ALK variant 3a I1171N/D1203N

    Plasmid
    #183833
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    I1171N/D1203N
    Promoter
    CMV
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    183833-plasmid-map-sequence-id-360485

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Showing: 4481 - 4500 of 5817 results