We narrowed to 9,355 results for: BLI

1. KG#865

   Plasmid

   #110912

   Purpose

   Coding sequence for attaching the Auxin Inducible Degron to the C-terminus of a protein followed by a 5X Myc tag with spacers

   Depositor

   Kenneth Miller

   Insert

   Auxin Inducible Degron-5X Myc-Spacers for C-terminal attachment
   

   Expression

   Bacterial

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. KG#883

   Plasmid

   #110913

   Purpose

   Coding sequence for attaching the a 5X Myc tag with spacers followed by the Auxin Inducible Degron to the N-terminus of a protein

   Depositor

   Kenneth Miller

   Insert

   5X Myc-Spacers-Auxin Inducible Degron for N-terminal attachment
   

   Expression

   Bacterial

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG-FNLS-T2A-EGFP-ires-puro

   Plasmid

   #121169

   Purpose

   CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.

   Depositor

   Timo Otonkoski

   Insert

   FNLS-T2A-EGFP-ires-puro
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. H11-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

   Plasmid

   #180157

   Purpose

   td-sfGFP dre reporter with Hipp11 recombineering homology arms

   Depositor

   Mario Capecchi

   Insert

   td-sfGFP
   

   Use

   Mouse Targeting; Dre/rox

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-GFAP-jGCaMP7c variant 1513

   Plasmid

   #173159

   Purpose

   Expresses jGCaMP7c variant 1513 in astrocytes

   Depositor

   Hervé Chneiweiss, Vincent Vialou

   Insert

   jGCaMP7c variant 1513-WPRE
   

   Use

   AAV

   Promoter

   GFAP

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)

   Plasmid

   #53205

   Purpose

   N-terminal chromosomal tagging with stable GFP under control of GAL promoter

   Depositor

   John Pringle

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   GFP (F64L, S65T, V163A) and kanMX6

   Promoter

   Gal1 promoter

   Available Since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

7. PTPN5

   Plasmid

   #38887

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PTPN5 (PTPN5 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   C496S inactivating mutation

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. H11-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

   Plasmid

   #180155

   Purpose

   td-sfGFP cre reporter with Hipp11 recombineering homology arms

   Depositor

   Mario Capecchi

   Insert

   td-sfGFP
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. RHOU

   Plasmid

   #38885

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   RHOU (RHOU Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   C191L

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pKG212

    Plasmid

    #69758

    Purpose

    Retroviral expression of CENP-T aa 1-242

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 1-242 (CENPT Human)
    

    Use

    Retroviral

    Tags

    LacI-NLS and mCherry

    Expression

    Mammalian

    Mutation

    e.coli optimized sequence

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pT7-7 asyn S87A/S129E

    Plasmid

    #36065

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein S87A/S129E (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    S87A/S129E

    Promoter

    T7

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFLAG-6a-Ataxin3Q22-UIM*

    Plasmid

    #22128

    Depositor

    Henry Paulson

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    UIM 1,2,3 SA-DG

    Available Since

    Nov. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pVP16-tev-PRT1

    Plasmid

    #83405

    Purpose

    bacterial expression vector pVP16 containing Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    

    Tags

    His8, MBP, and tev cleavage site

    Expression

    Bacterial

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. SDCBPA-c010

    Plasmid

    #175482

    Purpose

    Protein expression in bacterial cells. PDZ1 and PDZ2 domains, A106-V288. Can be used for crystallography.

    Depositor

    Opher Gileadi

    Insert

    SDCBP (SDCBP Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUASp YFP Cdc42 WT

    Plasmid

    #52248

    Purpose

    Drosophila expression of YFP-Cdc42

    Depositor

    Matthew Scott

    Insert

    Cdc42 (Cdc42 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

16. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid

    #121171

    Purpose

    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    xFNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

    Plasmid

    #121175

    Purpose

    Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines

    Depositor

    Timo Otonkoski

    Insert

    GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNtCp-rbcL-PHLS-aadA-accD

    Plasmid

    #52470

    Purpose

    Inserts the beta-phellandrene synthase gene and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacum

    Depositor

    Anastasios Melis

    Insert

    rbcL-PHLS-aadA-accD
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. PTGR2

    Plasmid

    #38949

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTGR2 (PTGR2 Human)
    

    Tags

    Tev His Flag

    Expression

    Bacterial

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pLV-Enh eGFP Reporter-muKC2-IGR13

    Plasmid

    #59282

    Purpose

    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.

    Depositor

    Benjamin Yu

    Insert

    17kb upstream krt5
    

    Use

    Lentiviral

    Promoter

    Mu Hsp68 minimal promoter

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only