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Plasmid#120242PurposeExpresses V5-tagged Ccdc167 in lentiviral vectorDepositorInsertCcdc167 (Ccdc167 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
IRES-EGFP-Flag-hRabin8
Plasmid#118073PurposeExpresses Flag-tagged human Rabin8 in mammalian cellsDepositorInserthuman Rabin8 (RAB3IP Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1 alphaAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_SpCas9MT3-FKBP_SL
Plasmid#107302PurposeExpresses R1335K mutant SpCas9 fused to FKBP in mammalian cellsDepositorInsertSpCas9
UseCRISPRTagsFKBPExpressionMammalianMutationR1335K and fused to FKBPPromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_FRB-TALETS3-3xFLAG-2xNLS
Plasmid#107306PurposeExpresses TALE-VEGFA-TS3 fused to FRB in mammalian cellsDepositorInsertTALE_VEGFA-TS3
UseCRISPRTags3x Flag, 2xNLS and FRBExpressionMammalianMutationPromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 CEP164 WW domain
Plasmid#104276PurposeBacterial expression of WW domain from CEP164DepositorInsertCEP164 WW domain (CEP164 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 CEP164 WW domain
Plasmid#104300PurposeBacterial expression of WW domain from CEP164DepositorInsertCEP164 WW domain (CEP164 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSIIKS_HIS3_I
Plasmid#109062Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.DepositorInsertHIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterHIS3Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pHH0103 HECW2 WW domain #1
Plasmid#104244PurposeBacterial expression of WW domain #1 from HECW2DepositorInsertHECW2 WW domain #1 (HECW2 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Trp-eK-Flv
Plasmid#83390Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Asp-eK-Flv
Plasmid#83389Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-His-eK-Flv
Plasmid#83388Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Lys-eK-Flv
Plasmid#83387Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Leu-eK-Flv
Plasmid#83386Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Arg-eK-Flv
Plasmid#83385Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Phe-eK-Flv
Plasmid#83384Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA1-gltA2-GapAP1
Plasmid#87150PurposegltA1-gltA2-GapAP1 targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertgltA1-gltA2-gapAP1 guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJM102: pHps2 RFP on pCM66T
Plasmid#74741PurposepCM66T with promoter pHps2 driving RFP expression (maxRFP = BBa_E1010)DepositorInsertRFP (BioBrick part number BBa_E1010)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpHps2 (extracted from Methylovorus glucosetrophus…Available sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK103
Plasmid#73265PurposeProduces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, C-terminal His6 tag (AaAarBH6)DepositorInserthypothetical acetic acid resistance protein B
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only