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We narrowed to 30,923 results for: Ide;

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Showing: 4481 - 4500 of 30923 results

  1. pSCBE3-HF1-Hypa

    Plasmid
    #218158
    Purpose
    This plasmid harbors the base editor SCBE3-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218158-plasmid-map-sequence-id-433272

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  2. pSCBE3-Hypa

    Plasmid
    #218157
    Purpose
    This plasmid harbors the base editor SCBE3-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N692A…
    Promoter
    rpsL promoter
    Available Since
    May 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    218157-plasmid-map-sequence-id-433172

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  6. Ir7f-T2A-QF2 HDR plasmid

    Plasmid
    #140942
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f gene
    Depositor
    Leslie Vosshall
    Article
    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12
    Insert
    Ir7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
    Mutation
    4 point mutations (annotated on plasmid map) were…
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140942-plasmid-map-sequence-id-313600

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  7. INCTbiosyn-p35S-GFP(rc)5

    Plasmid
    #127523
    Purpose
    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 5 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 5 attachment sites.
    Expression
    Plant
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127523-plasmid-map-sequence-id-250834

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  8. INCTbiosyn-p35S-GFP(rc)7

    Plasmid
    #127524
    Purpose
    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 7 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 7 attachment sites.
    Expression
    Plant
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127524-plasmid-map-sequence-id-250839

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  9. INCTbiosyn-p35S-GFP(rc)9

    Plasmid
    #127525
    Purpose
    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 9 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 9 attachment sites.
    Expression
    Plant
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127525-plasmid-map-sequence-id-250840

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  10. INCTbiosyn-pEF-GFP(rc)5

    Plasmid
    #127506
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 5 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 5 attachment sites.
    Expression
    Mammalian
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127506-plasmid-map-sequence-id-250745

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  11. INCTbiosyn-pEF-GFP(rc)7

    Plasmid
    #127507
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 7 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 7 attachment sites.
    Expression
    Mammalian
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127507-plasmid-map-sequence-id-250746

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  13. INCTbiosyn-p35S-GFP(rc)2

    Plasmid
    #127521
    Purpose
    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
    Expression
    Plant
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127521-plasmid-map-sequence-id-250828

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  14. INCTbiosyn-pEF-GFP(rc)4

    Plasmid
    #127505
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 4 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase 4 attachment sites.
    Expression
    Mammalian
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127505-plasmid-map-sequence-id-250744

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  17. pSH-Csy4-T2A-SpRFN

    Plasmid
    #85754
    Purpose
    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-dCas9
    Use
    CRISPR
    Tags
    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85754-plasmid-map-sequence-id-170272

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  18. INCTbiosyn-p35S(rc)2_4_5-GFP

    Plasmid
    #127520
    Purpose
    Plasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
    Expression
    Plant
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127520-plasmid-map-sequence-id-250826

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  20. pelB-MBP-HtrA-ss

    Plasmid
    #137066
    Purpose
    E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature HtrA: lacks the HtrA signal peptide
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137066-plasmid-map-sequence-id-291956

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Showing: 4481 - 4500 of 30923 results