We narrowed to 29,111 results for: PLE;

1. pSNAP-CAPZ

   Plasmid

   #69948

   Purpose

   Simultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZ

   Depositor

   Jeff Gelles

   Article

   Bombardier et al Nat Commun. 2015 Nov 13;6:8707. doi: 10.

   Inserts

   CapZ beta 1 (CAPZB Chicken)
   CapZ alpha 1 (CAPZA1 Chicken)
   

   Use

   Tags

   His 6 and SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBUN501

   Plasmid

   #50582

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   zCas9D10A
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Cas9D10A nickase, was derived from the zCas9 and …

   Promoter

   AtU6-26p and Ubi1p

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pL40C_PGKintron_Cas9_Green

   Plasmid

   #134966

   Purpose

   Lentiviral vector coding Cas9 and mNeonGreen

   Depositor

   Beat Bornhauser

   Article

   Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

   Insert

   Cas9-P2A-mNeonGreen
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK promoter with beta-globin intron

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTEE

   Plasmid

   #159748

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQCXIP mScarlet-MOSPD2 W201E

   Plasmid

   #186477

   Purpose

   Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Retroviral

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-UBC-S2-RAB_EKARev

   Plasmid

   #160726

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S2-RAB_EKARev
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

   Plasmid

   #111387

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   mCAR (Cxadr Mouse)
   ChETA
   

   Use

   AAV

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-UBC-S3-RAB_EKARev

   Plasmid

   #160727

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S3-RAB_EKARev
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-EGFP-C1-HsNot1iso1_1093-1317_W

   Plasmid

   #147892

   Purpose

   Mammalian Expression of HsNot1iso1_1093-1317 *Please note that this plasmid encodes amino acids 1093-1322 (not 1093-1317 as the name suggests).

   Depositor

   Elisa Izaurralde

   Article

   Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

   Insert

   HsNot1iso1_1093-1317 (CNOT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. UPRT-mCh-Nluc-P2A-neo-UPRT

    Plasmid

    #135015

    Purpose

    Expresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Inserts

    mCh-Nluc-P2A-neo
    UPRT 5'UTR
    UPRT 3'UTR
    

    Use

    Cryptosporidium expression

    Tags

    mCherry and Nluc-neo

    Expression

    Mutation

    Promoter

    Cryptosporidium actin and enolase

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-UBC-S1-jRCaMP1a

    Plasmid

    #160728

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S1-jRCaMP1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEMS1097

    Plasmid

    #29028

    Purpose

    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple201 (SLC6A5 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-Cre-NLS

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pATP416-­pluxO5-­ptetO7-­crtYBI

    Plasmid

    #165975

    Purpose

    Expresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTA

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Inserts

    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    C1281A, G1698A and C66A, C1359T

    Promoter

    Synthetic promoter (pluxO5), Synthetic promoter (…

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGAPTrap-eGFP-IRESMuro

    Plasmid

    #82504

    Purpose

    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    eGFP-IRESMuro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGAPTrap-Luc2-IRESMeo

    Plasmid

    #82509

    Purpose

    Vector targeting the Firefly Luciferase (Luc2) gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Meo gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    Luc2-IRESMeo
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS416 ATG5-E141D-HA-ATG12-ATG16

    Plasmid

    #166851

    Purpose

    Expresses mutant E141D ATG5, HA-ATG12 ATG16 in yeast.

    Depositor

    Daniel Klionsky

    Article

    Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

    Insert

    ATG5 ATG12 ATG16 (ATG5, ATG12, ATG16 Budding Yeast)
    

    Use

    Tags

    HA-ATG12

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEMS1430

    Plasmid

    #29225

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple170 (POGZ Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCEE

    Plasmid

    #159746

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGAPTrap-LacZ-IRESMuro

    Plasmid

    #82507

    Purpose

    Vector targeting the LacZ gene to the GAPDH locus of human cells. LacZ is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    LacZ-IRESMuro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. (attB) tdMCP-tagged BRaf with MS2-circRNA

    Plasmid

    #226163

    Purpose

    Used to integrate 3xFlag-tdMCP-BRaf and MS2-circRNA barcodes into RNA-Protein Landing Pad cell lines

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Inserts

    3xFlag-tdMCP-BRaf (BRAF Human)
    IRES-mCherry-P2A-PuroR
    MS2-circRNA barcode
    

    Use

    Bxb1 attb, no promoter

    Tags

    3xFlag-tdMCP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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