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pVITRO-HPV31 L1L2

Plasmid

#52600

Purpose

Expresses HPV31 L1 and L2 in mammalian cells

Depositor

Richard Roden

Publication

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV31 L1
HPV31 L2

Expression

Mammalian

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pENO1-dTom-NATr

Plasmid

#52208

Purpose

Constitutive expression of Candida codon-optimized dTomato with NAT selectable marker

Depositor

Robert Wheeler

Publication

Gratacap et al Dis Model Mech. 2013 Sep;6(5):1260-70. d

Insert

dTomato

Expression

Bacterial and Yeast

Mutation

codon optimized for Candida species

Promoter

ENO1

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pJKR-O-mphR

Plasmid

#62570

Purpose

Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Publication

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

mCherry

Expression

Bacterial

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pAGM4673

Plasmid

#48014

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pGATOR-DTA

Plasmid

#64103

Purpose

Tet inducible, Gateway adapted ROSA26 targeting Vector with DTA

Depositor

Bill Skarnes

Publication

Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

Insert

rTTA (Gt(ROSA)26Sor Synthetic)

Use

Mouse Targeting; Tet inducible

Expression

Mammalian

Promoter

ROSA26 endogenous

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLL3.7 K122 FH-TAZ-ires-GFP-Osteocalcin-promoter-H2B mCherry reporter

Plasmid

#68713

Depositor

Yutaka Hata

Insert

H2B mCherry and TAZ/WWTR1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV for TAZ/WWTR1

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only
pTK312

Plasmid

#59569

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Publication

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP XbaI attB1 Kozak EGFP ochre attB2 AscI

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dPlcR

Plasmid

#181832

Purpose

Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).

Depositor

Jin Zhang

Publication

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-TDsmURFP-RBS-HO-1

Plasmid

#80342

Purpose

Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial cells.

Depositor

Erik Rodriguez, Roger Tsien

Publication

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

Tandem Dimer smURFP
HO-1

Tags

6 Histidine Tag

Expression

Bacterial

Promoter

pBAD

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBluescriptII[attB/Ins1]

Plasmid

#74100

Purpose

"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

Depositor

Ben Szaro

Publication

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Phi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators

Expression

Bacterial

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJZC33

Plasmid

#62330

Purpose

sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2x MS2 binding module
MCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pRDA_887

Plasmid

#201164

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

Depositor

John Doench

Publication

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-GFP-V5-His

Plasmid

#85621

Purpose

Expresses GFP and V5 in Drosophila.

Depositor

Kenneth Irvine

Publication

Rauskolb et al PLoS Biol. 2011 Jun;9(6):e1000624. doi:

Insert

GFP

Tags

V5, His

Expression

Insect

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pHR_Gal4UAS_humanIL-2_T2A_MIP1α_PGK_mCherry

Plasmid

#85429

Purpose

Lentiviral vector for Gal4 inducible human IL-2/MIP1a with constitutive mCherry expression

Depositor

Wendell Lim

Publication

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Human IL-2 T2A MIPalpha

Use

Lentiviral

Expression

Mammalian

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLMB51

Plasmid

#40083

Depositor

Philip Poole

Publication

Tett et al Appl Environ Microbiol. 2012 Oct;78(19):

Inserts

gusA
tauR
tau promoter

Expression

Bacterial

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CMPE-ePPEplus

Plasmid

#205242

Purpose

For multiplex plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Publication

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS

Use

CRISPR

Expression

Plant

Mutation

Detailed in manuscript

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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NINJA TC

Plasmid

#159274

Purpose

Expresses the NINJA targeting construct (inducible neoantigen expression via cumulative Cre, rtTA, doxycycline and tamoxifen).

Depositor

Nikhil Joshi

Publication

Damo et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

NINJA TC

Use

Mouse Targeting

Expression

Mammalian

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-OTI-TCRB_p2_TCRA

Plasmid

#131034

Purpose

Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

Depositor

Jeff Hammerbacher

Publication

Aksoy et al bioRxiv

Inserts

TCRB (Tcrb Mouse)
TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV/T7

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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8027_GFP-KrasG12C_2B_puro

Plasmid

#64372

Purpose

This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with puro resistance gene

Depositor

Georgios Stathopoulos

Publication

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Mutation

changed glycine 12 to cysteine

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hCtRNA_FT

Plasmid

#186715

Purpose

Fragment template for DAP array

Depositor

Xue Gao

Publication

Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

Insert

gRNA scaffold and hCtRNA

Use

CRISPR

Expression

Mammalian

Promoter

hCtRNA

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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