Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 57,800 results for: plasmid

Clear keywords
Showing: 4481 - 4500 of 57800 results

  1. pET28-SpRY-NLS-6xHis (gbr2101)

    Plasmid
    #181743
    Purpose
    bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Publication
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    T7
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    181743-plasmid-map-sequence-id-354675

    Enlarge

  2. pCMV-Csy4-PE2

    Plasmid
    #172667
    Purpose
    Enhanced prime editing system in mammalian cells. This plasmid is used by PE3 and PE3b
    Depositor
    Xiaolong Wang
    Publication
    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-PE2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    172667-plasmid-map-sequence-id-339511

    Enlarge

  6. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

    Plasmid
    #139994
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139994-plasmid-map-sequence-id-275519

    Enlarge

  9. Ultra-Exon1Q23-Myc-A

    Plasmid
    #110486
    Purpose
    Lentiviral vector to express wild-type N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged wild type huntingtin exon1 fragment with 23 repeats of Q)
    Depositor
    Baoji Xu
    Publication
    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do
    Insert
    Homo sapiens huntingtin (HTT Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Short 3'UTR wild type huntingtin exon1 fragm…
    Promoter
    UbC
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    110486-plasmid-map-sequence-id-212584

    Enlarge

  12. pΔΦ

    Plasmid
    #60009
    Purpose
    Deleted foamy virus vector plasmid containing a backbone with a polylinker region for insertion of a transgene cassette. Ampicillin resistant.
    Depositor
    David Russell
    Publication
    Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.
    Type
    Empty backbone
    Use
    Deleted foamy virus
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    60009-plasmid-map-sequence-id-212300

    Enlarge

  19. EGFP-Rab8a-TN

    Plasmid
    #86077
    Purpose
    A mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.
    Depositor
    Lei Lu
    Publication
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hRab8A (RAB8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Changed threonine 22 to aspargine
    Promoter
    CMV
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    86077-plasmid-map-sequence-id-168966

    Enlarge

Showing: 4481 - 4500 of 57800 results