We narrowed to 6,963 results for: RAP

1. sgRNA1_IL1RN

   Plasmid

   #64140

   Purpose

   Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   IL1RN (IL1RN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgRNA3_IL1RN

   Plasmid

   #64151

   Purpose

   Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3_IL1RN (IL1RN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-X1-Neo-GFP-ATL2-deltaTM

   Plasmid

   #109021

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL2-deltaTM (ATL2 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   truncated form without C-terminal ER transmembran…

   Promoter

   EIF-1a promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA2_IL1RN

   Plasmid

   #64142

   Purpose

   Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2_IL1RN (IL1RN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. sgRNA4_IL1RN

   Plasmid

   #64152

   Purpose

   Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA4_IL1RN (IL1RN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SRAcode_in_pUC57_v3

   Plasmid

   #49822

   Purpose

   for transcribing SRA nucl 419-1356 of NM_001035235 with T7

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   steroid receptor RNA activator RNA coding (SRA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   KpnI site at 5' start of RNA coding seq

   Promoter

   T7

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCLASP_Crz1(19A)_CLASP_RGS2membrane

   Plasmid

   #133085

   Purpose

   Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   Crz1*-CLASP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   pADH1

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. SRAcode_in_pUC57_v3(767-1356)

   Plasmid

   #49826

   Purpose

   for transcribing SRA nucl 767-1356 of NM_001035235 with T7

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   steroid receptor RNA activator RNA coding (SRA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   KpnI site at 5' start of RNA coding seq

   Promoter

   T7

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEntr1a-mcherry-FKBP-TIAM1(DHPH)

   Plasmid

   #176135

   Purpose

   Heterodimerization with FRB (rapamycin), Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   See Depositor Comments Below

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2 Notch1 LNG-6MT

    Plasmid

    #41738

    Depositor

    Raphael Kopan, Jeffrey Nye

    Article

    Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

    Insert

    Notch-1 Lin-Notch-glp (LNG) repeat-containing construct (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 leader peptide (aa 1-23) …

    Promoter

    Available since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pJW1886

    Plasmid

    #154333

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I^SEC (Lox511I)^TEV::degron::3xFLAG
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(-)-HF2-BE2

    Plasmid

    #120396

    Purpose

    Expresses HF2-BE2 in mammalian cells

    Depositor

    Junjiu Huang

    Article

    Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

    Insert

    HF2-BE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mammalian codon-optimized Cas9-HF

    Promoter

    CMV

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF-HA-TSAd-d239-74

    Plasmid

    #84560

    Purpose

    mammalian expression of TSAd-d239-74

    Depositor

    Anne Spurkland

    Article

    Berge et al PLoS One. 2010 Mar 18;5(3):e9761. doi: 1

    Insert

    TSAd (SH2D2A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deletion of aa 239-274

    Promoter

    EF1a

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pCS2 Notch1 LNG (CC→SS)-6MT

    Plasmid

    #41739

    Depositor

    Raphael Kopan

    Article

    Mumm et al Mol Cell. 2000 Feb;5(2):197-206.

    Insert

    Notch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 leader peptide (aa 1-23) …

    Promoter

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

15. SRAcode_in_pUC57_v3(1000-1356)

    Plasmid

    #49827

    Purpose

    for transcribing SRA nucl 1000-1356 of NM_001035235 with T7

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator RNA coding (SRA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    KpnI site at 5' start of RNA coding seq

    Promoter

    T7

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR TRE_IL1Ra-PGK_BFP

    Plasmid

    #223549

    Purpose

    Inducible IL-1Ra; constitutive BFP

    Depositor

    Jonathan Brunger

    Article

    Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

    Inserts

    IL-1Ra (IL1RN Human)
    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MZB150

    Plasmid

    #217840

    Purpose

    Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (52-252) of Cytohesin-2 (CYTH2/ARNO) for rapid nucleotide exchange and activation of Arf GTPases

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    ARNO (CYTH2 Human)
    

    Use

    Tags

    10x His and SUMO (SMT3)

    Expression

    Bacterial

    Mutation

    Deleted residues 1-51 and 253-400

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. L2096-GPA-BRET-Biosensor-Retrovirus-TD146

    Plasmid

    #222878

    Purpose

    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-NanoLuc114-CyOFP1-T2A-FKBP-GPA-NanoLuc11S
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L2117-FKBP-GPA-114-RH-CyOFP1

    Plasmid

    #222884

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc114-RH-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L2116-FRB-GPA-114-RH-CyOFP1

    Plasmid

    #222883

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc114-RH-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. L2379-FKBP-GPA(V84R)-20-11S

    Plasmid

    #222901

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA(V84R)-20GS-NanoLuc11S
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    V84R in GPA

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. L2380-FKBP-GPA(V84R)-20-114

    Plasmid

    #222900

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA(V84R)-20GS-NanoLuc114
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    V84R in GPA

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. L2378-FRB-GPA(V84R)-20-11S

    Plasmid

    #222899

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA(V84R)-20GS-NanoLuc11S
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    V84R in GPA

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. L2377-FRB-GPA(V84R)-20-114

    Plasmid

    #222898

    Purpose

    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA(V84R)-20GS-NanoLuc114
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    V84R in GPA

    Promoter

    CMV

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135

    Plasmid

    #222875

    Purpose

    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA(WT)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. L2132-FKBP-GPA-114-5-CyOFP1

    Plasmid

    #222891

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc114-5GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. L2127-FRB-GPA-114-20-CyOFP1

    Plasmid

    #222886

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc114-20GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. L2129-FRB-GPA-114-5-CyOFP1

    Plasmid

    #222888

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc114-5GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. L2128-FRB-GPA-114-10-CyOFP1

    Plasmid

    #222887

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc114-10GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. L2131-FKBP-GPA-114-10-CyOFP1

    Plasmid

    #222890

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc114-10GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge