Addgene

We narrowed to 23,917 results for: nes

Showing: 9041 - 9080 of 23917 results

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pKDC.109

Plasmid

#227189

Purpose

T7 IPTG-driven Cas9

Depositor

Seth Shipman

Article

Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

Insert

SpyCas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAM292

Plasmid

#226129

Purpose

Expression of His6-SUMO-Ub-mEos3.2-intein-CBD

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-SUMO-Ub-mEos3.2-intein-CBD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM296

Plasmid

#226132

Purpose

Expression of Ufd1L55R-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Ufd1L55R-3C-His6

Use

Tags

Expression

Bacterial

Mutation

L55R

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-empty

Plasmid

#220203

Purpose

mini-Tn7 cloning vector - empty backbone

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM295

Plasmid

#226131

Purpose

Expression of Ufd1R64C-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Ufd1R64C-3C-His6

Use

Tags

Expression

Bacterial

Mutation

R64C

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pSpy

Plasmid

#220184

Purpose

sgRNA expression vector - promoterless backbone

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

promoterless backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

promoterless

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyA

Plasmid

#220185

Purpose

sgRNA expression vector - pEM7 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pEM7 promoter

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pEM7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyC

Plasmid

#220187

Purpose

sgRNA expression vector - pBG42 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBG42

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyD

Plasmid

#220188

Purpose

sgRNA expression vector - pBG28 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG28 promoter

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBG28

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p667-UBC-STK17A-V5-miniTurbo_IDG-K

Plasmid

#214466

Purpose

Gateway destination clone of STK17A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

STK17A-V5-miniTurbo (STK17A Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUCSh2-2300

Plasmid

#170984

Purpose

Encodes sgRNA target 2300 and Kanamycin resistance transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pUCSh3-2300

Plasmid

#170985

Purpose

Encodes sgRNA target 2300 and Tetracycline resistance transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRO16Sh5

Plasmid

#180076

Purpose

Expression of ShCAST under control of Plac with pRO1600 origin.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLac

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET30a- phage MazG-like/Atd1

Plasmid

#195244

Purpose

Expersses phage MazG-like/Atd1

Depositor

Douglas Feldman

Article

Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

Insert

phage MazG

Use

Tags

Flag and TwinStrep

Expression

Bacterial

Mutation

Catalytic site mutant

Promoter

T7

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos2_X

Plasmid

#147938

Purpose

Mammalian Expression of HsNanos2

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2 (NANOS2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNanos3_4-20-Y10E-Strep_W

Plasmid

#147827

Purpose

Bacterial Expression of HsNanos_4-20-Y10E

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos_4-20-Y10E

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpG-HsNanos2_6-22-Strep_W

Plasmid

#147828

Purpose

Bacterial Expression of HsNanos2_6-55

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2_6-55 (NANOS2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos1_X

Plasmid

#147931

Purpose

Mammalian Expression of HsNanos1

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1 (NANOS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsNanos2_P

Plasmid

#147199

Purpose

Mammalian Expression of HsNanos2

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2 (NANOS2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNanos1_P

Plasmid

#147201

Purpose

Mammalian Expression of HsNanos1

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1 (NANOS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNanos2_P

Plasmid

#147202

Purpose

Mammalian Expression of HsNanos2

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2 (NANOS2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1-Dm7SL-RNA-long_M

Plasmid

#146891

Purpose

Insect Expression of Dm7SL-RNA-long

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

Dm7SL-RNA-long (7SLRNA:CR32864 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1-Dm7SL-RNA-short_M

Plasmid

#146892

Purpose

Insect Expression of Dm7SL-RNA-short

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

Dm7SL-RNA-short (7SLRNA:CR32864 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_1-417_M

Plasmid

#146895

Purpose

Insect Expression of DmCup_1-417

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_1-417 (cup Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_1-779_M

Plasmid

#146902

Purpose

Insect Expression of DmCup_1-779

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_1-779 (cup Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCup_418-1132_M

Plasmid

#146906

Purpose

Insect Expression of DmCup_418-1132

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_418-1132 (cup Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCup-L379AL383A_M

Plasmid

#146912

Purpose

Insect Expression of DmCup-L379AL383A

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup-L379AL383A (cup Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-CS-DmCup_311-440_M

Plasmid

#146913

Purpose

Bacterial Expression of DmCup_311-440

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_311-440 (cup Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-V5-SBP-C1-HsNanos1_X

Plasmid

#147954

Purpose

Mammalian Expression of HsNanos1

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1 (NANOS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458-MAFB-sg

Plasmid

#194720

Purpose

px458 with guide RNA that target hMAFB

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

Cas9 (MAFB Streptococcus pyogenes)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG422 pBABE GFP-SPC24

Plasmid

#192696

Purpose

Retroviral transgene expression of human SPC24

Depositor

Iain Cheeseman

Article

Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

Insert

SPC24 (SPC24 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-V5-SBP-C1-HsNanos2_X

Plasmid

#147960

Purpose

Mammalian Expression of HsNanos2

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2 (NANOS2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
3xTRE DREaMR 3p3DsR

Plasmid

#182206

Purpose

3xTRE Tet-On cassette with 3xp3 DsRed maker for generating knockins with doxycycline-inducible rescue

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

rTetR-3XP3-DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1xTRE DREaMR 3p3DsR

Plasmid

#182204

Purpose

1xTRE Tet-On cassette with 3xp3 DsRed marker for generating knockins with doxycycline-inducible rescue.

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

rTetR-3XP3-DsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCup_771-1132_C

Plasmid

#146030

Purpose

Insect Expression of DmCup_771-1132

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_771-1132 (cup Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCup_771-1132_C

Plasmid

#146034

Purpose

Insect Expression of DmCup_771-1132

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_771-1132 (cup Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCup-Y342AL347A_C

Plasmid

#146035

Purpose

Insect Expression of DmCup-Y342AL347A

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup-Y342AL347A (cup Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lmajor_gp63_28_0570_WT

Plasmid

#171642

Purpose

Expression of wildtype L. major glcyoprotein-63 from chromosome 28 in mammalian cells

Depositor

Dennis Ko

Article

Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

Insert

LmjF.28.0570

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Lmajor_gp63_31_2000_WT

Plasmid

#171643

Purpose

Expression of wildtype L. major glcyoprotein-63 from chromosome 31 in mammalian cells

Depositor

Dennis Ko

Article

Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

Insert

LmjF.31.2000

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pH6HTC_PKM-Halo

Plasmid

#175321

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PKM (PKM Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 9041 - 9080 of 23917 results

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