We narrowed to 13,583 results for: TIM
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Plasmid#221131PurposeExpression of heterologous genes under alcohol induction optimised with ubi-Y-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.DepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus and …TagsExpressionMutationPromoterPalcA-T1-PalcS/M-T2-PaldA expression cassette (al…Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only
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pYFAC-CH2-ubi-M-pyrG
Plasmid#221132PurposeExpression of heterologous genes under alcohol induction optimised with ubi-M-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.DepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus and …TagsExpressionMutationPromoterPalcA-T1-PalcS/M-T2-PaldA expression cassette (al…Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5418_pMBP-GlcR
Plasmid#221192PurposeExpression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.DepositorInsertglcR (pden4400 from Paracoccus denitrificans)
UseTags10x His tag with E. coli maltose binding protein …ExpressionBacterialMutationPromoterT7 promoter and T7 promoter-lacOAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTGFR-DuET
Plasmid#213371PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertPTGFR (PTGFR Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast coUCK2 D62A
Plasmid#211530PurposeExpresses codon optimized catalytic mutant UCK2DepositorInsertUCK2 D62A (UCK2 Human)
UseRetroviralTagsExpressionMammalianMutationD62A (catalytic mutant)PromoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
opt.a_pSTC0-zeo
Plasmid#202347PurposeDesign opt.a (greedy optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsertopt.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
opt.b_pSTC0-zeo
Plasmid#202348PurposeDesign opt.b (parallel tempering optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsertopt.b
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast CG1358
Plasmid#218541PurposeDrosophila CG1358 cDNA (human codon optimized)DepositorInsertCG1358 (HisT Fly)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
MRGPRX4-DuET
Plasmid#213347PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertMRGPRX4 (MRGPRX4 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
MRGPRX2-DuET
Plasmid#213345PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertMRGPRX2 (MRGPRX2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
NPFF2-DuET
Plasmid#213352PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertNPFF2 (NPFFR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MLNR-DuET
Plasmid#213339PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertMLNR (MLNR Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
NMUR2-DuET
Plasmid#213350PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertNMUR2 (NMUR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
NPBW2-DuET
Plasmid#213351PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertNPBW2 (NPBWR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSTR4-DuET
Plasmid#213379PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertSSTR4 (SSTR4 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PK1-DuET
Plasmid#213366PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertPK1 (PROKR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PRRP-DuET
Plasmid#213367PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertPRRP (PRLHR Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTAFR-DuET
Plasmid#213368PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertPTAFR (PTAFR Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
QRFP-DuET
Plasmid#213372PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertQRFP (QRFP Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
P2RY8-DuET
Plasmid#213365PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertP2RY8 (P2RY8 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
NPY1R-DuET
Plasmid#213354PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertNPY1R (NPY1R Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTNR1B-DuET
Plasmid#213349PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertMTNR1B (MTNR1B Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
NPS-DuET
Plasmid#213353PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertNPS (NPS Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MRGPRG-DuET
Plasmid#213343PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertMRGPRG (MRGPRG Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSTR5-DuET
Plasmid#213380PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertSSTR5 (SSTR5 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SUCNR1-DuET
Plasmid#213381PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertSUCNR1 (SUCNR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
S1PR4-DuET
Plasmid#213375PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertS1PR4 (S1PR4 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSTR1-DuET
Plasmid#213376PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertSSTR1 (SSTR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTGER1-DuET
Plasmid#213370PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertPTGER1 (PTGER1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA_F164R238
Plasmid#216230PurposeS. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged arginine 238 to serine, phenylalanine 168…PromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_A467
Plasmid#216234PurposeS. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged alanine 467 to valinePromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_T522
Plasmid#216235PurposeS. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged threonine 522 to isoleucinePromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-EGFP
Plasmid#210378PurposeExpresses SARS-CoV-2 NSP4 fused with EGFP in mammalian cellsDepositorInsertNSP4 (ORF1ab SARS-CoV-2)
UseTagsEGFPExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP2-Flag
Plasmid#210344PurposeExpresses SARS-CoV-2 NSP2 in mammalian cellsDepositorInsertSARS-CoV-2 NSP2 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
UseTagsFlagExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-AncSZ
Plasmid#214235PurposeBacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylationDepositorInsertSyk kinase (SYK Human)
UseTags6xHisExpressionBacterialMutationSequence gained through ancestral reconstruction …PromoterT7Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP782_Traptavidin-KRAB-CO
Plasmid#211775PurposeAviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertSpyCatcher-DNMT3A
UseTags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP3C-mCherry
Plasmid#210377PurposeExpresses the C terminal of SARS-CoV-2 NSP3 fused with mCherry tag in mammalian cellsDepositorInsertNSP3C (ORF1ab SARS-CoV-2)
UseTagsmCherryExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP785_Traptavidin-DNMT3A-CO
Plasmid#211778PurposeAviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertTraptavidin-EZH2
UseTags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP781_SnoopCatcher-KRAB-CO
Plasmid#211774PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertTraptavidin-KRAB
UseTags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect KRAB e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) NAT marker (R) (SBE173)
Plasmid#195014Purposenourseothricin (NAT) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOSDepositorInsertpXYL+NAT+tNOS
UseTagsExpressionBacterialMutationPromoterpXYLAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) G418 marker (R) (SBE 137)
Plasmid#195011Purposegeneticin (G418) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOSDepositorInsertpXYL+G418+tNOS
UseTagsExpressionBacterialMutationPromoterpXYLAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p202-Syt1-C2A-wt
Plasmid#201553PurposeExpresses wild-type Octopus synaptotagmin-1 C2A domain in E. coliDepositorInsertSynaptotagmin-1 C2A domain (partial)
UseTagsExpressionBacterialMutationCodon-optimized for E. coliPromoterRBSAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_WT POLI
Plasmid#201440PurposeExpresses human DNA polymerase iota with an N-terminal HA tag in mammalian cellsDepositorInsertDNA polymerase iota (POLI Human)
UseTagsHAExpressionMammalianMutationCoding sequence has been optimised for expression…PromoterCMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJM919_WT His POLI
Plasmid#201444PurposeLow copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tagDepositorInsertDNA polymerase iota (POLI Human)
UseTags6x HisExpressionBacterialMutationCoding sequence has been optimised for expression…PromoterlacIqAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW pHluorin-SYT9 IRES mRuby3
Plasmid#195698PurposeLentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoterDepositorInsertSyt9 (Syt5 Rat)
UseLentiviralTagspHluorinExpressionMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt9 IRES mRuby3
Plasmid#195700PurposeLentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoterDepositorInsertSyt9 (Syt5 Mouse)
UseLentiviralTagsExpressionMutationencodes only the first 68 amino acids of Tac1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TEV-A7 (2-180)
Plasmid#190066PurposeCodon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain.DepositorInsertAnnexin A7 PRD (ANXA7 Human)
UseTagsGB1-His-TEVExpressionBacterialMutationPRD only, residues 2-180PromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TEV-A7-Strep (2-180)
Plasmid#190067PurposeCodon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.DepositorInsertAnnexin A7 PRD (ANXA7 Human)
UseTagsGB1-His-TEV and StrepExpressionBacterialMutationPRD only, residues 2-180PromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 4 CMV eGFP
Plasmid#206272PurposeENTR Vector 4 for MultiSite Gateway assembly. Encodes eGFP under the control of a CMV promoterDepositorInserteGFP
UseMultimate/gateway entr 4TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 4 CMV mTagBFP
Plasmid#206279PurposeENTR Vector 4 for MultiSite Gateway assembly. Encodes mTagBFP under the control of a CMV promoter.DepositorInsertmTagBFP
UseMultimate/gateway entr 4TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only