We narrowed to 23,099 results for: crispr

1. lentiGuide_puro-P2A-mNeonGreen

   Plasmid

   #221464

   Purpose

   Lentiviral expression of an sgRNA with mNeonGreen fluorescent marker

   Depositor

   James Eshleman

   Insert

   mNeonGreen
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6, EF-1a

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKSB- 3xP3-DsRedSV40 GGGG AAGA

   Plasmid

   #174532

   Purpose

   3xP3-DsRed fluorescence marker cassette for gene knock-in in mosquitoes

   Depositor

   Eric Marois

   Insert

   3xP3-DsRed BsaI cassette
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pY120 (pcDNA3.1-huFbCpf1)

   Plasmid

   #92296

   Purpose

   Expression of humanized FbCpf1

   Depositor

   Feng Zhang

   Insert

   huFbCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pY126 (pcDNA3.1-huBoCpf1)

   Plasmid

   #92302

   Purpose

   Expression of humanized BoCpf1

   Depositor

   Feng Zhang

   Insert

   huBoCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY123 (pcDNA3.1-huCMaCpf1)

   Plasmid

   #92299

   Purpose

   Expression of humanized CMaCpf1

   Depositor

   Feng Zhang

   Insert

   huCMaCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pY114 (pcDNA3.1-huPgCpf1)

   Plasmid

   #92273

   Purpose

   Expression of humanized PgCpf1

   Depositor

   Feng Zhang

   Insert

   huPgCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pY122 (pcDNA3.1-huCPbCpf1)

   Plasmid

   #92298

   Purpose

   Expression of humanized CPbCpf1

   Depositor

   Feng Zhang

   Insert

   huCPbCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUPD2 MS2 (GB2665)

   Plasmid

   #160597

   Purpose

   CDS of MS2 protein with B3 nomenclature

   Depositor

   Diego Orzaez

   Insert

   MS2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCASCADE

   Plasmid

   #65821

   Purpose

   Control guide RNA plasmid E. coli , low phosphate induction

   Depositor

   Michael Lynch

   Insert

   control guideRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E . coli ugpB gene promoter, low phosphate induc…

   Available Since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYJ09-lenti-EF1a-NKX6.1-puro

    Plasmid

    #131054

    Purpose

    Forced expression of NKX6.1

    Depositor

    Xiaojun Lian

    Insert

    NKX6.1 (NKX6-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKS1604

    Plasmid

    #239528

    Purpose

    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    ScYea4p-mTurquoise2-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKS1606

    Plasmid

    #239529

    Purpose

    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    ScYea4p-mTurquoise2-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKS1620

    Plasmid

    #239551

    Purpose

    Express GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrCPR-mTagBFP2-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKS1617

    Plasmid

    #239548

    Purpose

    Express GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrG8H-mTagBFP2-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKS1618

    Plasmid

    #239549

    Purpose

    Express GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrG8H-mTagBFP2-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKS1619

    Plasmid

    #239550

    Purpose

    Express GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrCPR-mTagBFP2-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKS1601

    Plasmid

    #239526

    Purpose

    Express CpPT1(52-408) membrane enzyme cargo and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKS1531

    Plasmid

    #239521

    Purpose

    Express truncated VoGES and VvGT14 in the peroxisome for synthesis of geranyl _-glucoside; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    tVoGES-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HCP9

    Plasmid

    #166111

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2

    Depositor

    Matthias Heinemann

    Insert

    Hta2-sg18 (HTA2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pROS15_MATz

    Plasmid

    #166100

    Purpose

    Expression of dual gRNA targeting the MAT locus

    Depositor

    Rebecca Burgess

    Insert

    MAT Z1
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

    Plasmid

    #67977

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid

    #60231

    Purpose

    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    EGFP
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA-P2A and EGFP-KASH

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hSyn

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKLV2-EF1a-BsdCas9-W

    Plasmid

    #67978

    Purpose

    Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-Cas9 cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Cas9 fused with Flag-tag and a NLS at the N termi…

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid

    #60230

    Purpose

    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    EGFP
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA-P2A and EGFP-KASH

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hSyn

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

    Plasmid

    #67974

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2ABFP cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

    Plasmid

    #67982

    Purpose

    Cas9 activity reporter with mCherry and GFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Plasmid

    #179105

    Purpose

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Plasmid

    #179106

    Purpose

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60226

    Purpose

    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Renilla luciferase
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

    Tags

    Cre-HA and Rluc-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKLV2-EF1a-Cas9Bsd-W

    Plasmid

    #68343

    Purpose

    Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-Cas9 cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Cas9 fused with Flag-tag and a NLS at the N termi…

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

    Plasmid

    #67976

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmAG cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid

    #61364

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

    Plasmid

    #67986

    Purpose

    Cas9 activity reporter with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60225

    Purpose

    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Renilla luciferase
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

    Tags

    Cre-HA and Rluc-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available Since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAGmCherry-gRNA

    Plasmid

    #87110

    Purpose

    gRNA cloning vector with CAGmCherry

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

    Plasmid

    #223123

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9=D10A

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-T7-TadCBEd-SpG-P2A-EGFP (BKS777/AHK571)

    Plasmid

    #223124

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpG with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpG-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpG=D10A/D1135L/S1136W/G1218…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

    Plasmid

    #67975

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AZsG cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

    Plasmid

    #67984

    Purpose

    Cas9 activity reporter with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

    Plasmid

    #67990

    Purpose

    CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

    Plasmid

    #67983

    Purpose

    Cas9 activity reporter (control) with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

    Plasmid

    #113158

    Purpose

    An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid

    #61359

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

    Plasmid

    #113156

    Purpose

    An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat Rosa26
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid

    #61363

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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46. GCN5 FL-dCas9-p300 core

    Plasmid

    #179554

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pOTTC1079 - pAAV TH gRNA A EF1a EGFP

    Plasmid

    #113159

    Purpose

    An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

    Plasmid

    #113155

    Purpose

    An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

    Plasmid

    #113157

    Purpose

    An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat MANF
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCMV-T7 RNA POL

    Plasmid

    #138352

    Purpose

    Expressing T7 RNA Polymerase in mammalian cells

    Depositor

    Baisong Lu

    Insert

    T7 RNA POL
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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