162,717 results

1. PX333-T2A-EGFP

   Plasmid

   #197417

   Purpose

   SpCas9-T2A-EGFP with dual sgRNA cloning backbones

   Depositor

   Yeh-Hsing Lao

   Article

   Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh; U6

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-mTagBFP2-PLCδ-PH

   Plasmid

   #214275

   Purpose

   Mammalian expression of blue fluorescent PI(4,5)P2 reporter mTagBFP2-PLCδ-PH

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   mTagBFP2-PLCδ(1-175) (PLCD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6691 Bicistronic_ires_puro

   Plasmid

   #64335

   Purpose

   This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-CFP-SaCas9-HF (without sgRNA)

   Plasmid

   #134470

   Purpose

   Human expression vector for SaCas9-HF variant.

   Depositor

   Zongli Zheng

   Article

   Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

   Insert

   Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
   

   Use

   CRISPR

   Tags

   SV40NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   R245A, N413A, N419A, R654A

   Promoter

   CAG

   Available since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-3'TwStrep

   Plasmid

   #208618

   Purpose

   Empty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tag

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   Tags

   Twin Strep

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB1H2w2-zif268

   Plasmid

   #18045

   Depositor

   Scot Wolfe

   Article

   Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

   Insert

   zif268
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Zif268 is fused to the omega subunit of RNA polym…

   Promoter

   Available since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherryC1 Human WIP

   Plasmid

   #29573

   Depositor

   Anna Huttenlocher

   Article

   Cortesio et al Mol Biol Cell. 2010 Jan 1. 21(1):186-97.

   Insert

   WASP-interacting protein (WIPF1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. Myc-RILPL1

   Plasmid

   #201470

   Purpose

   For overexpression in cultured cells to visualize RILPL1 protein interactions.

   Depositor

   Suzanne Pfeffer

   Article

   Pal et al Sci Adv. 2023 Dec 15;9(50):eadj1205. doi

   Insert

   RILPL1 (RILPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCellFree_KA1_SARS-COV-2_S

   Plasmid

   #169402

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus S with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _S (S SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Expression

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. N1-mSin1.1-GFP

    Plasmid

    #72907

    Purpose

    GFP-tagged mSin1 (isoform 1)

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    mSin1.1 (MAPKAP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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