161,242 results

1. pEHAC1-HA-BirAonly

   Plasmid

   #232584

   Purpose

   Express HA-BirA

   Depositor

   Sagar Bhogaraju

   Article

   Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

   Insert

   BirA
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPP085

   Plasmid

   #158795

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPRyl

   Plasmid

   #70007

   Purpose

   CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2015 Dec 29.

   Inserts

   Codon optimized Cas9 from S. pyogenes
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-APEX2-C Cloning Vector

   Plasmid

   #128146

   Purpose

   To express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEM

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   Flag-APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-hSTING-EGFP

   Plasmid

   #214149

   Purpose

   Retroviral transduction of EGFP-tagged STING

   Depositor

   Nan Yan

   Article

   Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

   Insert

   STING (STING1 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PPRE-pNL1.3

   Plasmid

   #84394

   Purpose

   Secreted Luciferase reporter for PPARgamma activity

   Depositor

   Severine Degrelle, Thierry Fournier

   Article

   Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

   Insert

   PPARG response element (PPARG Human)
   

   Use

   Tags

   secNluc

   Expression

   Mammalian

   Mutation

   Promoter

   PPRE

   Available since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO-MCS

   Plasmid

   #185594

   Purpose

   Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N1 Full length Importin beta

   Plasmid

   #106941

   Purpose

   Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

   Depositor

   Patrizia Lavia

   Article

   Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

   Insert

   human Importin beta-1 (KPNB1) (KPNB1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CLYBL-TO-hNGN2-BSD-mApple

   Plasmid

   #124229

   Purpose

   Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

   Depositor

   Michael Ward

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   NGN2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-FRT/TO-3*Flag-APEX2 C-term PML

    Plasmid

    #187583

    Purpose

    Express FLAG epitope and APEX2-tagged PML fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    PML (PML Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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